##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AhdI Aor51HI AscI AspEI
AviII AvrII BamHI BbeI BbrPI
BglI BlnI BlpI Bpu1102I BsgI
BsiWI BspDI BssHII Bst1107I BstZ17I
CelII ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I FseI FspI
KasI KspI MluI NaeI NarI
NgoMIV NotI NruI PacI PaeR7I
PmaCI PmeI PmlI PshAI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
StuI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 5746 TCCGGA T^CCGGA
AclI 2976 AACGTT AA^CGTT
AcyI 1330 GGCGTC G[GA]^CG[CT]C
AgeI 6722 ACCGGT A^CCGGT
Alw44I 1429 GTGCAC G^TGCAC
AocI 6009 CCTGAGG CC^T[ACGT]AGG
ApaLI 1429 GTGCAC G^TGCAC
AspI 2139 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3365 CCCGAG C^[CT]CG[GA]G
BalI 549 TGGCCA TGG^CCA
BcgI 352 CGAGACTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 2039 CCGGG CC^[GC]GG
BglII 6258 AGATCT A^GATCT
BsaHI 1330 GGCGTC G[GA]^CG[CT]C
BseAI 5746 TCCGGA T^CCGGA
BsiEI 6368 CGATCG CG[GA][CT]^CG
BsoBI 3365 CCCGAG C^[CT]CG[GA]G
BspEI 5746 TCCGGA T^CCGGA
BsrBI 6362 CCGCTC CCGCTC(-3/-3)
BsrFI 6722 ACCGGT [GA]^CCGG[CT]
BssSI 4993 CACGAG CACGAG(-5/-1)
BstXI 3412 CCAAATTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 6009 CCTGAGG CC^T[ACGT]AGG
CfoI 7761 GCGC GCG^C
Cfr10I 6722 ACCGGT [GA]^CCGG[CT]
DrdI 6575 GACAGCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 6009 CCTGAGG CC^T[ACGT]AGG
EcoNI 7031 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 7760 AGCGCC [GA]GCGC^[CT]
HgaI 1331 GCGTC GACGC(5/10)
HhaI 7761 GCGC GCG^C
Hin1I 1330 GGCGTC G[GA]^CG[CT]C
HinP1I 7761 GCGC G^CGC
HpaI 6308 GTTAAC GTT^AAC
MluNI 549 TGGCCA TGG^CCA
MroI 5746 TCCGGA T^CCGGA
MscI 549 TGGCCA TGG^CCA
NciI 2039 CCGGG CC^[GC]GG
PflFI 2139 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 6891 CCATTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 6722 ACCGGT A^CCGGT
Psp1406I 2976 AACGTT AA^CGTT
PvuI 6368 CGATCG CGAT^CG
SphI 5315 GCATGC GCATG^C
SwaI 988 ATTTAAAT ATTT^AAAT
Tth111I 2139 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 6891 CCATTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 2690 GGTACC G^GTACC
6861 GGTACC
AccII 1 CGCG CG^CG
6125 CGCG
AseI 7071 ATTAAT AT^TAAT
8106 ATTAAT
AsnI 7071 ATTAAT AT^TAAT
8106 ATTAAT
Asp718I 2690 GGTACC G^GTACC
6861 GGTACC
BanI 2690 GGTACC G^G[CT][GA]CC
6861 GGTACC
BsaAI 5351 CACGTA [CT]AC^GT[GA]
5904 TACGTG
BsaBI 2994 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3351 GATCCATATC
BseRI 1263 GAGGAG GAGGAG(10/8)
4367 GAGGAG
BsmBI 1335 CGTCTC CGTCTC(1/5)
1345 CGTCTC
Bsp1407I 125 TGTACA T^GTACA
6374 TGTACA
BspMI 4572 ACCTGC ACCTGC(4/8)
4521 GCAGGT
BsrGI 125 TGTACA T^GTACA
6374 TGTACA
BstAPI 3054 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3063 GCAGCTTCTGC
BstEII 4353 GGTCACC G^GT[ACGT]ACC
5841 GGTCACC
BstPI 4353 GGTCACC G^GT[ACGT]ACC
5841 GGTCACC
BstUI 1 CGCG CG^CG
6125 CGCG
DraIII 1939 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
4802 CACACAGTG
EcoO65I 4353 GGTCACC G^GT[ACGT]ACC
5841 GGTCACC
EcoRV 3372 GATATC GAT^ATC
5481 GATATC
EcoT22I 5085 ATGCAT ATGCA^T
8015 ATGCAT
FauI 75 CCCGC CCCGC(4/6)
83 GCGGG
KpnI 2690 GGTACC GGTAC^C
6861 GGTACC
MamI 2994 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3351 GATCCATATC
MvnI 1 CGCG CG^CG
6125 CGCG
NheI 834 GCTAGC G^CTAGC
6879 GCTAGC
NsiI 5085 ATGCAT ATGCA^T
8015 ATGCAT
PpuMI 3524 GGGACCC [GA]G^G[AT]CC[CT]
7607 GGGTCCT
PshBI 7071 ATTAAT AT^TAAT
8106 ATTAAT
SapI 912 GAAGAGC GCTCTTC(1/4)
7636 GAAGAGC
ScaI 1207 AGTACT AGT^ACT
6169 AGTACT
SspBI 125 TGTACA T^GTACA
6374 TGTACA
XbaI 4934 TCTAGA T^CTAGA
6704 TCTAGA
XcmI 1723 CCATTGATAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
7940 CCAGATGTATCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApaI
ApoI Asp700I AspHI AvaII BanII
BbsI BbvCI BbvI BciVI BclI
BfaI BfrI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BspLU11I BsrDI BsrI BssKI
BstBI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MspA1I MspI MunI MvaI MwoI
NcoI NdeI NdeII NlaIII NlaIV
NspI NspV PleI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfuI SmlI
SpeI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI