##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BglII BlnI BsaHI BsaI
BseAI BseRI BsgI BsiEI BsiWI
BsmFI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HindIII HpaI KasI KpnI
KspI MluI MroI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PpuMI PshAI PstI PvuI PvuII
RcaI RsrII SacII SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AclI 1989 AACGTT AA^CGTT
AflII 1721 CTTAAG C^TTAAG
AlwNI 488 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 2214 ATTAAT AT^TAAT
AsnI 2214 ATTAAT AT^TAAT
AspHI 1892 GAGCTC G[AT]GC[AT]^C
AspI 545 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 988 TGGCCA TGG^CCA
BanII 1892 GAGCTC G[GA]GC[CT]^C
BbsI 2325 GTCTTC GAAGAC(2/6)
BfrI 1721 CTTAAG C^TTAAG
BpuAI 2325 GTCTTC GAAGAC(2/6)
BsaWI 822 TCCGGT [AT]^CCGG[AT]
BsiHKAI 1892 GAGCTC G[AT]GC[AT]^C
BsmBI 531 GAGACG CGTCTC(1/5)
Bsp1407I 2035 TGTACA T^GTACA
BsrFI 454 GCCGGC [GA]^CCGG[CT]
BsrGI 2035 TGTACA T^GTACA
BstAPI 1546 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 454 GCCGGC [GA]^CCGG[CT]
DraII 383 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 991 CCATGG C^C[GA][CT]GG
EarI 67 CTCTTC CTCTTC(1/4)
EcoO109I 383 AGGCCCC [GA]G^G[ACGT]CC[CT]
HaeII 2138 GGCGCT [GA]GCGC^[CT]
HgaI 545 GACGC GACGC(5/10)
HincII 445 GTCGAC GT[CT]^[GA]AC
HindII 445 GTCGAC GT[CT]^[GA]AC
Ksp632I 67 CTCTTC CTCTTC(1/4)
MfeI 3 CAATTG C^AATTG
MluNI 988 TGGCCA TGG^CCA
MscI 988 TGGCCA TGG^CCA
MunI 3 CAATTG C^AATTG
NaeI 454 GCCGGC GCC^GGC
NcoI 991 CCATGG C^CATGG
NgoMIV 454 GCCGGC G^CCGGC
NspI 468 ACATGC [GA]CATG^[CT]
PaeR7I 72 CTCGAG C^TCGAG
PflFI 545 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2214 ATTAAT AT^TAAT
Psp1406I 1989 AACGTT AA^CGTT
SacI 1892 GAGCTC GAGCT^C
SalI 445 GTCGAC G^TCGAC
SgrAI 453 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 2035 TGTACA T^GTACA
Tth111I 545 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 929 TCTAGA T^CTAGA
XcmI 706 CCAACCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 72 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 72 CTCGAG C^[CT]CG[GA]G
349 CTCGGG
AvaII 126 GGACC G^G[AT]CC
882 GGACC
BcnI 148 CCGGG CC^[GC]GG
355 CCGGG
BglI 147 GCCGGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
271 GCCTCGGCGGC
BlpI 493 GCTGAGC GC^T[ACGT]AGC
2278 GCTGAGC
BpmI 408 CTGGAG CTGGAG(16/14)
528 CTGGAG
Bpu1102I 493 GCTGAGC GC^T[ACGT]AGC
2278 GCTGAGC
BsaAI 839 TACGTG [CT]AC^GT[GA]
2238 CACGTA
BsoBI 72 CTCGAG C^[CT]CG[GA]G
349 CTCGGG
CelII 493 GCTGAGC GC^T[ACGT]AGC
2278 GCTGAGC
EaeI 477 CGGCCA [CT]^GGCC[GA]
988 TGGCCA
EcoT14I 991 CCATGG C^C[AT][AT]GG
1802 CCTTGG
MspA1I 328 CAGCGG C[AC]G^C[GT]G
1964 CCGCTG
NciI 148 CCGGG CC^[GC]GG
355 CCGGG
PflMI 710 CCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2129 CCAGTATTTGG
PleI 2046 GACTC GAGTC(4/5)
2338 GACTC
SfcI 771 CTGTAG C^T[GA][CT]AG
918 CTGTAG
SspI 663 AATATT AAT^ATT
1108 AATATT
StyI 991 CCATGG C^C[AT][AT]GG
1802 CCTTGG
TfiI 1253 GAATC G^A[AT]TC
1466 GAATC
Van91I 710 CCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2129 CCAGTATTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI BbvI BfaI
BmyI BsaBI BsaJI BsiYI BslI
BsmAI BsmI Bsp1286I BsrI BssHII
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII