##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII Acc65I AclI AgeI Alw44I
ApaLI AscI Asp718I AviII AvrII
BbeI BbrPI BlnI BsiWI BspHI
BssHII BssSI Bst1107I BstBI BstZ17I
CpoI DraI FseI FspI HpaI
KasI KpnI MluI NarI NheI
NotI NspV PacI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SbfI SfiI SfoI SfuI
SgrAI SnaBI SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AcyI 7053 GACGCC G[GA]^CG[CT]C
Aor51HI 6673 AGCGCT AGC^GCT
BsaHI 7053 GACGCC G[GA]^CG[CT]C
BsiEI 203 CGGCCG CG[GA][CT]^CG
BsmBI 119 CGTCTC CGTCTC(1/5)
BsmI 4864 GAATGC GAATGC(1/-1)
Bsp1407I 1634 TGTACA T^GTACA
BspDI 4978 ATCGAT AT^CGAT
BsrGI 1634 TGTACA T^GTACA
BstAPI 1532 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 6043 CCACCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 4978 ATCGAT AT^CGAT
DraIII 1391 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1299 GACCAACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 203 CGGCCG C^GGCCG
EclXI 203 CGGCCG C^GGCCG
Eco47III 6673 AGCGCT AGC^GCT
Eco52I 203 CGGCCG C^GGCCG
EcoRI 7064 GAATTC G^AATTC
EcoT22I 5798 ATGCAT ATGCA^T
Hin1I 7053 GACGCC G[GA]^CG[CT]C
KspI 324 CCGCGG CCGC^GG
NaeI 243 GCCGGC GCC^GGC
NcoI 6877 CCATGG C^CATGG
NdeI 4339 CATATG CA^TATG
NgoMIV 243 GCCGGC G^CCGGC
NruI 171 TCGCGA TCG^CGA
NsiI 5798 ATGCAT ATGCA^T
PaeR7I 775 CTCGAG C^TCGAG
PshAI 2159 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 324 CCGCGG CCGC^GG
SalI 4707 GTCGAC G^TCGAC
SapI 1931 GAAGAGC GCTCTTC(1/4)
SexAI 5354 ACCTGGT A^CC[AT]GGT
SpeI 7114 ACTAGT A^CTAGT
SspBI 1634 TGTACA T^GTACA
XhoI 775 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 6754 TCCGGA T^CCGGA
7005 TCCGGA
AflII 2424 CTTAAG C^TTAAG
3347 CTTAAG
ApaI 147 GGGCCC GGGCC^C
1787 GGGCCC
AseI 4616 ATTAAT AT^TAAT
7674 ATTAAT
AsnI 4616 ATTAAT AT^TAAT
7674 ATTAAT
AspI 4271 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6519 GACGCAGTC
BamHI 5021 GGATCC G^GATCC
5408 GGATCC
BciVI 1744 GTATCC GTATCC(6/5)
2834 GTATCC
BfrI 2424 CTTAAG C^TTAAG
3347 CTTAAG
BglII 3324 AGATCT A^GATCT
3374 AGATCT
BsaAI 1779 TACGTG [CT]AC^GT[GA]
5049 TACGTG
BseAI 6754 TCCGGA T^CCGGA
7005 TCCGGA
BspEI 6754 TCCGGA T^CCGGA
7005 TCCGGA
BsrFI 243 GCCGGC [GA]^CCGG[CT]
474 ACCGGC
BstEII 386 GGTGACC G^GT[ACGT]ACC
5428 GGTTACC
BstPI 386 GGTGACC G^GT[ACGT]ACC
5428 GGTTACC
Cfr10I 243 GCCGGC [GA]^CCGG[CT]
474 ACCGGC
EcoNI 1084 CCTTTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5526 CCTGAAACAGG
EcoO65I 386 GGTGACC G^GT[ACGT]ACC
5428 GGTTACC
EcoRV 4303 GATATC GAT^ATC
6888 GATATC
MfeI 2452 CAATTG C^AATTG
2958 CAATTG
MroI 6754 TCCGGA T^CCGGA
7005 TCCGGA
MunI 2452 CAATTG C^AATTG
2958 CAATTG
PflFI 4271 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6519 GACGCAGTC
PshBI 4616 ATTAAT AT^TAAT
7674 ATTAAT
ScaI 1882 AGTACT AGT^ACT
2512 AGTACT
SmaI 1790 CCCGGG CCC^GGG
4951 CCCGGG
SspI 4111 AATATT AAT^ATT
7513 AATATT
Tth111I 4271 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6519 GACGCAGTC
XbaI 3587 TCTAGA T^CTAGA
4028 TCTAGA
XmaCI 1790 CCCGGG C^CCGGG
4951 CCCGGG
XmaI 1790 CCCGGG C^CCGGG
4951 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AhdI AluI AlwI AlwNI
AocI ApoI Asp700I AspEI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcgI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaBI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrBI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI Eco81I EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI