##################################
# NO CUT ENZYMES ( 40 enzymes) #
##################################
AatII AflII AgeI AseI AsnI
BamHI BbeI BclI BfrI BsiWI
BspDI Bst1107I BstBI BstZ17I ClaI
DrdI EcoRV FbaI FseI KasI
MfeI MluI MunI NarI NdeI
NotI NruI NspV PacI PinAI
PmeI PshBI PvuI SfiI SfoI
SfuI SgrAI SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AccI 3569 GTCGAC GT^[AC][GT]AC
AclI 3158 AACGTT AA^CGTT
Aor51HI 1600 AGCGCT AGC^GCT
AscI 397 GGCGCGCC GG^CGCGCC
AviII 125 TGCGCA TGC^GCA
AvrII 3449 CCTAGG C^CTAGG
BlnI 3449 CCTAGG C^CTAGG
BsaI 2137 GAGACC GGTCTC(1/5)
BspLU11I 6800 ACATGT A^CATGT
BssSI 12 CTCGTG CACGAG(-5/-1)
CpoI 2733 CGGTCCG CG^G[AT]CCG
DraIII 6854 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1600 AGCGCT AGC^GCT
EcoNI 3552 CCTGGGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 125 TGCGCA TGC^GCA
HindIII 3936 AAGCTT A^AGCTT
KspI 6765 CCGCGG CCGC^GG
NaeI 1045 GCCGGC GCC^GGC
NgoMIV 1045 GCCGGC G^CCGGC
Psp1406I 3158 AACGTT AA^CGTT
RsrII 2733 CGGTCCG CG^G[AT]CCG
SacII 6765 CCGCGG CCGC^GG
SalI 3569 GTCGAC G^TCGAC
SbfI 2373 CCTGCAGG CCTGCA^GG
SexAI 5736 ACCAGGT A^CC[AT]GGT
Sse8387I 2373 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 827 GGTACC G^GTACC
952 GGTACC
AccIII 774 TCCGGA T^CCGGA
5533 TCCGGA
AcyI 2160 GACGCC G[GA]^CG[CT]C
3409 GACGCC
AhdI 3409 GACGCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6442 GACTGATGGTC
AocI 551 CCTTAGG CC^T[ACGT]AGG
822 CCTGAGG
ApaI 1444 GGGCCC GGGCC^C
2367 GGGCCC
Asp700I 1674 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5303 GAAACTTTTC
Asp718I 827 GGTACC G^GTACC
952 GGTACC
AspEI 3409 GACGCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6442 GACTGATGGTC
BbvCI 4063 CCTCAGC CCTCAGC(-5/-2)
1603 GCTGAGG
BciVI 5876 GGATAC GTATCC(6/5)
998 GTATCC
BsaBI 3654 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4627 GATGTAAATC
BsaHI 2160 GACGCC G[GA]^CG[CT]C
3409 GACGCC
BseAI 774 TCCGGA T^CCGGA
5533 TCCGGA
BspEI 774 TCCGGA T^CCGGA
5533 TCCGGA
BspHI 4615 TCATGA T^CATGA
6435 TCATGA
BssHII 398 GCGCGC G^CGCGC
416 GCGCGC
BstEII 1398 GGTCACC G^GT[ACGT]ACC
3031 GGTCACC
BstPI 1398 GGTCACC G^GT[ACGT]ACC
3031 GGTCACC
BstXI 1559 CCAGCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5050 CCATTACAGTGG
Bsu36I 551 CCTTAGG CC^T[ACGT]AGG
822 CCTGAGG
Eam1105I 3409 GACGCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6442 GACTGATGGTC
Eco81I 551 CCTTAGG CC^T[ACGT]AGG
822 CCTGAGG
EcoO65I 1398 GGTCACC G^GT[ACGT]ACC
3031 GGTCACC
EcoRI 1818 GAATTC G^AATTC
6190 GAATTC
Hin1I 2160 GACGCC G[GA]^CG[CT]C
3409 GACGCC
HpaI 5271 GTTAAC GTT^AAC
6717 GTTAAC
KpnI 827 GGTACC GGTAC^C
952 GGTACC
MamI 3654 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4627 GATGTAAATC
MroI 774 TCCGGA T^CCGGA
5533 TCCGGA
NheI 4133 GCTAGC G^CTAGC
6025 GCTAGC
PshAI 2067 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6491 GACTGCTGTC
RcaI 4615 TCATGA T^CATGA
6435 TCATGA
XmnI 1674 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5303 GAAACTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 158 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AspI AvaI AvaII
BalI BanI BanII BbrPI BbsI
BbvI BcgI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PaeR7I PflFI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmaI SmlI SphI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI