################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## Acc65I AccIII AclI AflII AflIII AgeI ApaI AscI Asp718I BbrPI BfrI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I BsrBI BsrFI Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI DraIII EagI EclXI Eco52I FseI HindIII HpaI KpnI MamI MluI MroI NaeI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SalI SapI SbfI SexAI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 5492 GACGTC GACGT^C Aor51HI 552 AGCGCT AGC^GCT Asp700I 5599 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 3750 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1336 TGCGCA TGC^GCA AvrII 5740 CCTAGG C^CTAGG BalI 732 TGGCCA TGG^CCA BamHI 1136 GGATCC G^GATCC BcgI 183 GCACCGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 4315 GGATAC GTATCC(6/5) BclI 5211 TGATCA T^GATCA BglII 3854 AGATCT A^GATCT BlnI 5740 CCTAGG C^CTAGG BlpI 638 GCTGAGC GC^T[ACGT]AGC Bpu1102I 638 GCTGAGC GC^T[ACGT]AGC BsmI 539 GAATGC GAATGC(1/-1) Bsp1407I 4188 TGTACA T^GTACA BspMI 1283 ACCTGC ACCTGC(4/8) BsrGI 4188 TGTACA T^GTACA BssHII 23 GCGCGC G^CGCGC BstEII 873 GGTGACC G^GT[ACGT]ACC BstPI 873 GGTGACC G^GT[ACGT]ACC CelII 638 GCTGAGC GC^T[ACGT]AGC Eco47III 552 AGCGCT AGC^GCT EcoO65I 873 GGTGACC G^GT[ACGT]ACC EcoRV 6242 GATATC GAT^ATC FbaI 5211 TGATCA T^GATCA FspI 1336 TGCGCA TGC^GCA HgaI 827 GCGTC GACGC(5/10) KspI 223 CCGCGG CCGC^GG MfeI 5715 CAATTG C^AATTG MluNI 732 TGGCCA TGG^CCA MscI 732 TGGCCA TGG^CCA MunI 5715 CAATTG C^AATTG NheI 5456 GCTAGC G^CTAGC PflFI 3750 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 523 CCAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacII 223 CCGCGG CCGC^GG ScaI 5438 AGTACT AGT^ACT SfiI 218 GGCCGCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 4188 TGTACA T^GTACA TaqI 5856 TCGA T^CGA Tth111I 3750 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 5856 TCGA T^CGA Van91I 523 CCAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 5599 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AhdI 3723 GACTTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4836 GACCAGATGTC Alw44I 181 GTGCAC G^TGCAC 1207 GTGCAC ApaLI 181 GTGCAC G^TGCAC 1207 GTGCAC AspEI 3723 GACTTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4836 GACCAGATGTC AvaII 328 GGACC G^G[AT]CC 1302 GGACC BbeI 71 GGCGCC GGCGC^C 1955 GGCGCC BsaI 755 GAGACC GGTCTC(1/5) 6398 GAGACC BssSI 375 CACGAG CACGAG(-5/-1) 6381 CACGAG DraI 2995 TTTAAA TTT^AAA 6106 TTTAAA Eam1105I 3723 GACTTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4836 GACCAGATGTC HincII 343 GTCAAC GT[CT]^[GA]AC 451 GTCAAC HindII 343 GTCAAC GT[CT]^[GA]AC 451 GTCAAC KasI 71 GGCGCC G^GCGCC 1955 GGCGCC NarI 71 GGCGCC GG^CGCC 1955 GGCGCC NcoI 720 CCATGG C^CATGG 1117 CCATGG PstI 1269 CTGCAG CTGCA^G 4026 CTGCAG SacI 598 GAGCTC GAGCT^C 2897 GAGCTC SfoI 71 GGCGCC GGC^GCC 1955 GGCGCC SphI 998 GCATGC GCATG^C 1846 GCATGC XbaI 658 TCTAGA T^CTAGA 3933 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApoI AseI AsnI AspHI AvaI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeI NdeII NlaIII NlaIV NsiI NspI PleI PshBI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII XmaCI XmaI