##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI ApaI AscI Asp718I BbrPI
BfrI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspLU11I BsrBI BsrFI Bst1107I BstBI
BstZ17I Cfr10I ClaI CpoI DraIII
EagI EclXI Eco52I FseI HindIII
HpaI KpnI MamI MluI MroI
NaeI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RsrII SalI SapI SbfI SexAI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 5492 GACGTC GACGT^C
Aor51HI 552 AGCGCT AGC^GCT
Asp700I 5599 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3750 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1336 TGCGCA TGC^GCA
AvrII 5740 CCTAGG C^CTAGG
BalI 732 TGGCCA TGG^CCA
BamHI 1136 GGATCC G^GATCC
BcgI 183 GCACCGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4315 GGATAC GTATCC(6/5)
BclI 5211 TGATCA T^GATCA
BglII 3854 AGATCT A^GATCT
BlnI 5740 CCTAGG C^CTAGG
BlpI 638 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 638 GCTGAGC GC^T[ACGT]AGC
BsmI 539 GAATGC GAATGC(1/-1)
Bsp1407I 4188 TGTACA T^GTACA
BspMI 1283 ACCTGC ACCTGC(4/8)
BsrGI 4188 TGTACA T^GTACA
BssHII 23 GCGCGC G^CGCGC
BstEII 873 GGTGACC G^GT[ACGT]ACC
BstPI 873 GGTGACC G^GT[ACGT]ACC
CelII 638 GCTGAGC GC^T[ACGT]AGC
Eco47III 552 AGCGCT AGC^GCT
EcoO65I 873 GGTGACC G^GT[ACGT]ACC
EcoRV 6242 GATATC GAT^ATC
FbaI 5211 TGATCA T^GATCA
FspI 1336 TGCGCA TGC^GCA
HgaI 827 GCGTC GACGC(5/10)
KspI 223 CCGCGG CCGC^GG
MfeI 5715 CAATTG C^AATTG
MluNI 732 TGGCCA TGG^CCA
MscI 732 TGGCCA TGG^CCA
MunI 5715 CAATTG C^AATTG
NheI 5456 GCTAGC G^CTAGC
PflFI 3750 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 523 CCAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 223 CCGCGG CCGC^GG
ScaI 5438 AGTACT AGT^ACT
SfiI 218 GGCCGCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 4188 TGTACA T^GTACA
TaqI 5856 TCGA T^CGA
Tth111I 3750 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 5856 TCGA T^CGA
Van91I 523 CCAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 5599 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AhdI 3723 GACTTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4836 GACCAGATGTC
Alw44I 181 GTGCAC G^TGCAC
1207 GTGCAC
ApaLI 181 GTGCAC G^TGCAC
1207 GTGCAC
AspEI 3723 GACTTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4836 GACCAGATGTC
AvaII 328 GGACC G^G[AT]CC
1302 GGACC
BbeI 71 GGCGCC GGCGC^C
1955 GGCGCC
BsaI 755 GAGACC GGTCTC(1/5)
6398 GAGACC
BssSI 375 CACGAG CACGAG(-5/-1)
6381 CACGAG
DraI 2995 TTTAAA TTT^AAA
6106 TTTAAA
Eam1105I 3723 GACTTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4836 GACCAGATGTC
HincII 343 GTCAAC GT[CT]^[GA]AC
451 GTCAAC
HindII 343 GTCAAC GT[CT]^[GA]AC
451 GTCAAC
KasI 71 GGCGCC G^GCGCC
1955 GGCGCC
NarI 71 GGCGCC GG^CGCC
1955 GGCGCC
NcoI 720 CCATGG C^CATGG
1117 CCATGG
PstI 1269 CTGCAG CTGCA^G
4026 CTGCAG
SacI 598 GAGCTC GAGCT^C
2897 GAGCTC
SfoI 71 GGCGCC GGC^GCC
1955 GGCGCC
SphI 998 GCATGC GCATG^C
1846 GCATGC
XbaI 658 TCTAGA T^CTAGA
3933 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApoI AseI AsnI AspHI AvaI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeI
NdeII NlaIII NlaIV NsiI NspI
PleI PshBI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII XmaCI XmaI