################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## Acc65I AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp718I AspEI AspI AviII BsiEI BsiWI BssHII CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I FseI FspI HpaI KpnI KspI MfeI MluI MunI NheI NotI NruI PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SgrAI SmaI SnaBI SpeI Sse8387I StuI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 1608 GACGTC GACGT^C AccIII 2031 TCCGGA T^CCGGA Asp700I 3088 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 2667 TGGCCA TGG^CCA BbeI 2994 GGCGCC GGCGC^C BbvCI 4891 GCTGAGG CCTCAGC(-5/-2) BclI 6408 TGATCA T^GATCA BsaBI 1040 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 2031 TCCGGA T^CCGGA BsmBI 4821 CGTCTC CGTCTC(1/5) BsmI 679 GAATGC GAATGC(1/-1) Bsp1407I 2922 TGTACA T^GTACA BspDI 3182 ATCGAT AT^CGAT BspEI 2031 TCCGGA T^CCGGA BsrBI 1480 CCGCTC CCGCTC(-3/-3) BsrGI 2922 TGTACA T^GTACA BstAPI 4923 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3736 TTCGAA TT^CGAA BstEII 3328 GGTGACC G^GT[ACGT]ACC BstPI 3328 GGTGACC G^GT[ACGT]ACC BstXI 2104 CCAGATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 3182 ATCGAT AT^CGAT DrdI 1608 GACGTCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 3328 GGTGACC G^GT[ACGT]ACC EcoRI 3007 GAATTC G^AATTC EcoRV 2306 GATATC GAT^ATC FbaI 6408 TGATCA T^GATCA HindIII 964 AAGCTT A^AGCTT KasI 2994 GGCGCC G^GCGCC MamI 1040 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2667 TGGCCA TGG^CCA MroI 2031 TCCGGA T^CCGGA MscI 2667 TGGCCA TGG^CCA NaeI 2204 GCCGGC GCC^GGC NarI 2994 GGCGCC GG^CGCC NdeI 340 CATATG CA^TATG NgoMIV 2204 GCCGGC G^CCGGC NspV 3736 TTCGAA TT^CGAA PflMI 961 CCAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SapI 3560 GCTCTTC GCTCTTC(1/4) SfoI 2994 GGCGCC GGC^GCC SfuI 3736 TTCGAA TT^CGAA SspBI 2922 TGTACA T^GTACA SwaI 4548 ATTTAAAT ATTT^AAAT Van91I 961 CCAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 3088 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccI 13 GTATAC GT^[AC][GT]AC 3296 GTATAC AcyI 1608 GACGTC G[GA]^CG[CT]C 2994 GGCGCC AflII 5452 CTTAAG C^TTAAG 5890 CTTAAG AocI 1441 CCTGAGG CC^T[ACGT]AGG 4683 CCTAAGG AvaI 1552 CCCGAG C^[CT]CG[GA]G 2515 CTCGGG AvrII 4799 CCTAGG C^CTAGG 4965 CCTAGG BbrPI 1262 CACGTG CAC^GTG 3609 CACGTG BcgI 4897 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 6092 GCAGGAGGATCG BfrI 5452 CTTAAG C^TTAAG 5890 CTTAAG BlnI 4799 CCTAGG C^CTAGG 4965 CCTAGG BlpI 1088 GCTGAGC GC^T[ACGT]AGC 2962 GCTGAGC Bpu1102I 1088 GCTGAGC GC^T[ACGT]AGC 2962 GCTGAGC BsaAI 1262 CACGTG [CT]AC^GT[GA] 3609 CACGTG BsaHI 1608 GACGTC G[GA]^CG[CT]C 2994 GGCGCC BsaWI 2031 TCCGGA [AT]^CCGG[AT] 3378 ACCGGA BsoBI 1552 CCCGAG C^[CT]CG[GA]G 2515 CTCGGG BspLU11I 5169 ACATGT A^CATGT 6429 ACATGT BssSI 3851 CACGAG CACGAG(-5/-1) 1727 CTCGTG Bst1107I 13 GTATAC GTA^TAC 3296 GTATAC BstZ17I 13 GTATAC GTA^TAC 3296 GTATAC Bsu36I 1441 CCTGAGG CC^T[ACGT]AGG 4683 CCTAAGG CelII 1088 GCTGAGC GC^T[ACGT]AGC 2962 GCTGAGC Eco81I 1441 CCTGAGG CC^T[ACGT]AGG 4683 CCTAAGG EcoT22I 2246 ATGCAT ATGCA^T 5105 ATGCAT HaeII 2994 GGCGCC [GA]GCGC^[CT] 3034 GGCGCT HgaI 1715 GCGTC GACGC(5/10) 5728 GCGTC Hin1I 1608 GACGTC G[GA]^CG[CT]C 2994 GGCGCC HincII 3374 GTCAAC GT[CT]^[GA]AC 6487 GTTGAC HindII 3374 GTCAAC GT[CT]^[GA]AC 6487 GTTGAC NsiI 2246 ATGCAT ATGCA^T 5105 ATGCAT PmaCI 1262 CACGTG CAC^GTG 3609 CACGTG PmlI 1262 CACGTG CAC^GTG 3609 CACGTG ScaI 2787 AGTACT AGT^ACT 5373 AGTACT SphI 4860 GCATGC GCATG^C 6047 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApaI ApoI AseI AsnI AspHI AvaII BamHI BanI BanII BbsI BbvI BciVI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PshBI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII