##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AclI AgeI AhdI Alw44I
Aor51HI ApaLI AscI Asp718I AspEI
AspI AviII BsiEI BsiWI BssHII
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I FseI FspI HpaI
KpnI KspI MfeI MluI MunI
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmeI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SgrAI SmaI SnaBI
SpeI Sse8387I StuI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 1608 GACGTC GACGT^C
AccIII 2031 TCCGGA T^CCGGA
Asp700I 3088 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2667 TGGCCA TGG^CCA
BbeI 2994 GGCGCC GGCGC^C
BbvCI 4891 GCTGAGG CCTCAGC(-5/-2)
BclI 6408 TGATCA T^GATCA
BsaBI 1040 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2031 TCCGGA T^CCGGA
BsmBI 4821 CGTCTC CGTCTC(1/5)
BsmI 679 GAATGC GAATGC(1/-1)
Bsp1407I 2922 TGTACA T^GTACA
BspDI 3182 ATCGAT AT^CGAT
BspEI 2031 TCCGGA T^CCGGA
BsrBI 1480 CCGCTC CCGCTC(-3/-3)
BsrGI 2922 TGTACA T^GTACA
BstAPI 4923 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3736 TTCGAA TT^CGAA
BstEII 3328 GGTGACC G^GT[ACGT]ACC
BstPI 3328 GGTGACC G^GT[ACGT]ACC
BstXI 2104 CCAGATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 3182 ATCGAT AT^CGAT
DrdI 1608 GACGTCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3328 GGTGACC G^GT[ACGT]ACC
EcoRI 3007 GAATTC G^AATTC
EcoRV 2306 GATATC GAT^ATC
FbaI 6408 TGATCA T^GATCA
HindIII 964 AAGCTT A^AGCTT
KasI 2994 GGCGCC G^GCGCC
MamI 1040 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2667 TGGCCA TGG^CCA
MroI 2031 TCCGGA T^CCGGA
MscI 2667 TGGCCA TGG^CCA
NaeI 2204 GCCGGC GCC^GGC
NarI 2994 GGCGCC GG^CGCC
NdeI 340 CATATG CA^TATG
NgoMIV 2204 GCCGGC G^CCGGC
NspV 3736 TTCGAA TT^CGAA
PflMI 961 CCAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 3560 GCTCTTC GCTCTTC(1/4)
SfoI 2994 GGCGCC GGC^GCC
SfuI 3736 TTCGAA TT^CGAA
SspBI 2922 TGTACA T^GTACA
SwaI 4548 ATTTAAAT ATTT^AAAT
Van91I 961 CCAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3088 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 13 GTATAC GT^[AC][GT]AC
3296 GTATAC
AcyI 1608 GACGTC G[GA]^CG[CT]C
2994 GGCGCC
AflII 5452 CTTAAG C^TTAAG
5890 CTTAAG
AocI 1441 CCTGAGG CC^T[ACGT]AGG
4683 CCTAAGG
AvaI 1552 CCCGAG C^[CT]CG[GA]G
2515 CTCGGG
AvrII 4799 CCTAGG C^CTAGG
4965 CCTAGG
BbrPI 1262 CACGTG CAC^GTG
3609 CACGTG
BcgI 4897 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
6092 GCAGGAGGATCG
BfrI 5452 CTTAAG C^TTAAG
5890 CTTAAG
BlnI 4799 CCTAGG C^CTAGG
4965 CCTAGG
BlpI 1088 GCTGAGC GC^T[ACGT]AGC
2962 GCTGAGC
Bpu1102I 1088 GCTGAGC GC^T[ACGT]AGC
2962 GCTGAGC
BsaAI 1262 CACGTG [CT]AC^GT[GA]
3609 CACGTG
BsaHI 1608 GACGTC G[GA]^CG[CT]C
2994 GGCGCC
BsaWI 2031 TCCGGA [AT]^CCGG[AT]
3378 ACCGGA
BsoBI 1552 CCCGAG C^[CT]CG[GA]G
2515 CTCGGG
BspLU11I 5169 ACATGT A^CATGT
6429 ACATGT
BssSI 3851 CACGAG CACGAG(-5/-1)
1727 CTCGTG
Bst1107I 13 GTATAC GTA^TAC
3296 GTATAC
BstZ17I 13 GTATAC GTA^TAC
3296 GTATAC
Bsu36I 1441 CCTGAGG CC^T[ACGT]AGG
4683 CCTAAGG
CelII 1088 GCTGAGC GC^T[ACGT]AGC
2962 GCTGAGC
Eco81I 1441 CCTGAGG CC^T[ACGT]AGG
4683 CCTAAGG
EcoT22I 2246 ATGCAT ATGCA^T
5105 ATGCAT
HaeII 2994 GGCGCC [GA]GCGC^[CT]
3034 GGCGCT
HgaI 1715 GCGTC GACGC(5/10)
5728 GCGTC
Hin1I 1608 GACGTC G[GA]^CG[CT]C
2994 GGCGCC
HincII 3374 GTCAAC GT[CT]^[GA]AC
6487 GTTGAC
HindII 3374 GTCAAC GT[CT]^[GA]AC
6487 GTTGAC
NsiI 2246 ATGCAT ATGCA^T
5105 ATGCAT
PmaCI 1262 CACGTG CAC^GTG
3609 CACGTG
PmlI 1262 CACGTG CAC^GTG
3609 CACGTG
ScaI 2787 AGTACT AGT^ACT
5373 AGTACT
SphI 4860 GCATGC GCATG^C
6047 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AseI AsnI AspHI AvaII BamHI
BanI BanII BbsI BbvI BciVI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PshBI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII