##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AviII AvrII
BbrPI BciVI BglI BlnI BlpI
Bpu1102I BsiEI BsiWI BspDI BsrBI
BssHII BstAPI BstBI BstEII BstPI
CelII ClaI CpoI DraIII DrdI
DsaI EagI EclXI Eco47III Eco52I
EcoNI EcoO65I FseI FspI KpnI
KspI MfeI MluI MunI NaeI
NcoI NgoMIV NotI NruI NspV
PaeR7I PmaCI PmeI PmlI PpuMI
PstI PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI SphI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 4345 CGCG CG^CG
AccIII 4694 TCCGGA T^CCGGA
AcyI 2255 GGCGCC G[GA]^CG[CT]C
AgeI 1203 ACCGGT A^CCGGT
AhdI 1009 GACTTGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 5607 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 1009 GACTTGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4076 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2255 GGCGCC GGCGC^C
BcgI 1617 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 3144 CACGTA [CT]AC^GT[GA]
BsaHI 2255 GGCGCC G[GA]^CG[CT]C
BseAI 4694 TCCGGA T^CCGGA
BsgI 6519 GTGCAG GTGCAG(16/14)
BspEI 4694 TCCGGA T^CCGGA
BspMI 388 GCAGGT ACCTGC(4/8)
BsrFI 1203 ACCGGT [GA]^CCGG[CT]
BssSI 1494 CACGAG CACGAG(-5/-1)
Bst1107I 1971 GTATAC GTA^TAC
BstUI 4345 CGCG CG^CG
BstZ17I 1971 GTATAC GTA^TAC
Cfr10I 1203 ACCGGT [GA]^CCGG[CT]
DraII 2529 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 1009 GACTTGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 2529 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 2085 GATATC GAT^ATC
EcoT22I 5412 ATGCAT ATGCA^T
FauI 4115 GCGGG CCCGC(4/6)
HgaI 33 GACGC GACGC(5/10)
Hin1I 2255 GGCGCC G[GA]^CG[CT]C
KasI 2255 GGCGCC G^GCGCC
MroI 4694 TCCGGA T^CCGGA
MspA1I 4817 CAGCTG C[AC]G^C[GT]G
MvnI 4345 CGCG CG^CG
NarI 2255 GGCGCC GG^CGCC
NheI 6198 GCTAGC G^CTAGC
NsiI 5412 ATGCAT ATGCA^T
PacI 567 TTAATTAA TTAAT^TAA
PflFI 4076 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 5606 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1203 ACCGGT A^CCGGT
PshAI 2144 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 4817 CAGCTG CAG^CTG
SfoI 2255 GGCGCC GGC^GCC
SpeI 1238 ACTAGT A^CTAGT
Tth111I 4076 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 5606 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 341 CCAACATCATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AocI 2677 CCTCAGG CC^T[ACGT]AGG
6077 CCTCAGG
AvaII 1592 GGTCC G^G[AT]CC
4692 GGTCC
BanII 111 GAGCTC G[GA]GC[CT]^C
5442 GAGCTC
BclI 1160 TGATCA T^GATCA
2349 TGATCA
BsaBI 1843 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5250 GATGTAAATC
BsaWI 1203 ACCGGT [AT]^CCGG[AT]
4694 TCCGGA
BseRI 5353 CTCCTC GAGGAG(10/8)
108 GAGGAG
BsmBI 1541 CGTCTC CGTCTC(1/5)
6031 GAGACG
Bsu36I 2677 CCTCAGG CC^T[ACGT]AGG
6077 CCTCAGG
Eco81I 2677 CCTCAGG CC^T[ACGT]AGG
6077 CCTCAGG
EcoT14I 2259 CCAAGG C^C[AT][AT]GG
4304 CCTTGG
FbaI 1160 TGATCA T^GATCA
2349 TGATCA
HaeII 2255 GGCGCC [GA]GCGC^[CT]
2471 GGCGCT
MamI 1843 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5250 GATGTAAATC
SacI 111 GAGCTC GAGCT^C
5442 GAGCTC
ScaI 3798 AGTACT AGT^ACT
5012 AGTACT
SmaI 1635 CCCGGG CCC^GGG
4146 CCCGGG
StuI 273 AGGCCT AGG^CCT
1584 AGGCCT
StyI 2259 CCAAGG C^C[AT][AT]GG
4304 CCTTGG
XmaCI 1635 CCCGGG C^CCGGG
4146 CCCGGG
XmaI 1635 CCCGGG C^CCGGG
4146 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AclI AcsI AfaI
AflII AflIII AluI AlwI ApoI
AseI AsnI Asp700I AspHI AvaI
BalI BamHI BanI BbsI BbvCI
BbvI BcnI BfaI BfrI BglII
BmyI BpmI BpuAI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspHI
BspLU11I BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EaeI EarI EcoRI EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NciI NdeI
NdeII NlaIII NlaIV NspI PleI
PshBI Psp1406I RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII XmnI