##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AclI AcyI AgeI AscI
AseI AsnI BbeI BcgI BlpI
Bpu1102I BsaHI BsiWI BspDI BsrFI
BssHII BstAPI BstBI BstEII BstPI
CelII Cfr10I ClaI CpoI EagI
EclXI Eco52I EcoO65I FseI Hin1I
HpaI KasI MfeI MluI MunI
NaeI NarI NgoMIV NotI NruI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
SbfI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI XbaI XcmI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 6561 GTATAC GT^[AC][GT]AC
AflII 4345 CTTAAG C^TTAAG
AhdI 531 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1618 GTGCAC G^TGCAC
Aor51HI 3537 AGCGCT AGC^GCT
ApaI 6454 GGGCCC GGGCC^C
ApaLI 1618 GTGCAC G^TGCAC
AspEI 531 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 6210 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 6597 CCTAGG C^CTAGG
BalI 6379 TGGCCA TGG^CCA
BamHI 6424 GGATCC G^GATCC
BfrI 4345 CTTAAG C^TTAAG
BglII 3986 AGATCT A^GATCT
BlnI 6597 CCTAGG C^CTAGG
BsaBI 3159 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 78 CGGTCG CG[GA][CT]^CG
Bst1107I 6561 GTATAC GTA^TAC
BstZ17I 6561 GTATAC GTA^TAC
DrdI 71 GACAGCCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 6379 TGGCCA [CT]^GGCC[GA]
Eam1105I 531 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3537 AGCGCT AGC^GCT
EcoRI 2313 GAATTC G^AATTC
EcoRV 6616 GATATC GAT^ATC
HaeII 3537 AGCGCT [GA]GCGC^[CT]
HgaI 41 GACGC GACGC(5/10)
HincII 165 GTTGAC GT[CT]^[GA]AC
HindII 165 GTTGAC GT[CT]^[GA]AC
HindIII 3318 AAGCTT A^AGCTT
KspI 17 CCGCGG CCGC^GG
MamI 3159 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 6379 TGGCCA TGG^CCA
MscI 6379 TGGCCA TGG^CCA
NheI 4434 GCTAGC G^CTAGC
PaeR7I 1198 CTCGAG C^TCGAG
PflFI 6210 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 17 CCGCGG CCGC^GG
SfiI 3473 GGCCAGCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 4815 AATATT AAT^ATT
Tth111I 6210 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1198 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 18 CGCG CG^CG
4544 CGCG
AccIII 37 TCCGGA T^CCGGA
5094 TCCGGA
AviII 1065 TGCGCA TGC^GCA
5686 TGCGCA
BglI 96 GCCCAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3474 GCCAGCAAGGC
BseAI 37 TCCGGA T^CCGGA
5094 TCCGGA
BspEI 37 TCCGGA T^CCGGA
5094 TCCGGA
BsrBI 56 CCGCTC CCGCTC(-3/-3)
501 CCGCTC
BssSI 805 CACGAG CACGAG(-5/-1)
4584 CACGAG
BstUI 18 CGCG CG^CG
4544 CGCG
BstXI 4254 CCAGGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4451 CCAGTGCTGTGG
EcoNI 2925 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3488 CCTGAGCCAGG
FauI 49 GCGGG CCCGC(4/6)
4438 GCGGG
FspI 1065 TGCGCA TGC^GCA
5686 TGCGCA
MroI 37 TCCGGA T^CCGGA
5094 TCCGGA
MvnI 18 CGCG CG^CG
4544 CGCG
ScaI 1868 AGTACT AGT^ACT
2222 AGTACT
SexAI 3288 ACCAGGT A^CC[AT]GGT
4382 ACCAGGT
SmaI 1751 CCCGGG CCC^GGG
5000 CCCGGG
SphI 2755 GCATGC GCATG^C
3043 GCATGC
XmaCI 1751 CCCGGG C^CCGGG
5000 CCCGGG
XmaI 1751 CCCGGG C^CCGGG
5000 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I Asp718I AspHI AvaI AvaII
BanI BanII BbrPI BbsI BbvCI
BbvI BciVI BclI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspHI
BspLU11I BspMI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EarI
Eco81I EcoO109I EcoRII EcoT14I EcoT22I
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RcaI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI