##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AclI AcyI AflII AflIII
AhdI Aor51HI AscI Asp700I AspEI
AviII AvrII BbeI BcgI BfrI
BglI BlnI BsaBI BsaHI BsiWI
BspDI BspLU11I BssHII BstEII BstPI
ClaI CpoI Eam1105I Eco47III EcoO65I
EcoRI FseI FspI HaeII Hin1I
KasI MamI MluI NarI NdeI
NotI PacI PaeR7I PmeI Psp1406I
PvuI RsrII SalI SapI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SphI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 4973 GGTACC G^GTACC
AccIII 1345 TCCGGA T^CCGGA
AgeI 5300 ACCGGT A^CCGGT
AocI 3994 CCTTAGG CC^T[ACGT]AGG
Asp718I 4973 GGTACC G^GTACC
AspI 809 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 6134 TGGCCA TGG^CCA
BbrPI 617 CACGTG CAC^GTG
BciVI 3108 GGATAC GTATCC(6/5)
BglII 6363 AGATCT A^GATCT
BsaAI 617 CACGTG [CT]AC^GT[GA]
BseAI 1345 TCCGGA T^CCGGA
BsiEI 121 CGGCCG CG[GA][CT]^CG
BsmBI 5393 CGTCTC CGTCTC(1/5)
BspEI 1345 TCCGGA T^CCGGA
BssSI 509 CTCGTG CACGAG(-5/-1)
BstBI 577 TTCGAA TT^CGAA
Bsu36I 3994 CCTTAGG CC^T[ACGT]AGG
DraIII 5603 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 121 CGGCCG C^GGCCG
EclXI 121 CGGCCG C^GGCCG
Eco52I 121 CGGCCG C^GGCCG
Eco81I 3994 CCTTAGG CC^T[ACGT]AGG
EcoRV 5368 GATATC GAT^ATC
EcoT22I 3370 ATGCAT ATGCA^T
KpnI 4973 GGTACC GGTAC^C
MfeI 1878 CAATTG C^AATTG
MluNI 6134 TGGCCA TGG^CCA
MroI 1345 TCCGGA T^CCGGA
MscI 6134 TGGCCA TGG^CCA
MunI 1878 CAATTG C^AATTG
NaeI 29 GCCGGC GCC^GGC
NgoMIV 29 GCCGGC G^CCGGC
NheI 3056 GCTAGC G^CTAGC
NsiI 3370 ATGCAT ATGCA^T
NspV 577 TTCGAA TT^CGAA
PflFI 809 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2417 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 5300 ACCGGT A^CCGGT
PmaCI 617 CACGTG CAC^GTG
PmlI 617 CACGTG CAC^GTG
PshAI 1843 GACTAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1229 GAGCTC GAGCT^C
SbfI 4058 CCTGCAGG CCTGCA^GG
ScaI 582 AGTACT AGT^ACT
SfuI 577 TTCGAA TT^CGAA
Sse8387I 4058 CCTGCAGG CCTGCA^GG
StuI 5760 AGGCCT AGG^CCT
SwaI 1810 ATTTAAAT ATTT^AAAT
Tth111I 809 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2417 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3313 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Alw44I 1944 GTGCAC G^TGCAC
2639 GTGCAC
ApaI 151 GGGCCC GGGCC^C
378 GGGCCC
ApaLI 1944 GTGCAC G^TGCAC
2639 GTGCAC
AseI 2709 ATTAAT AT^TAAT
4869 ATTAAT
AsnI 2709 ATTAAT AT^TAAT
4869 ATTAAT
BbvCI 3063 GCTGAGG CCTCAGC(-5/-2)
4191 GCTGAGG
BclI 936 TGATCA T^GATCA
6218 TGATCA
BsmI 6839 GAATGC GAATGC(1/-1)
4457 GCATTC
BspHI 1239 TCATGA T^CATGA
3711 TCATGA
BsrBI 3892 CCGCTC CCGCTC(-3/-3)
86 GAGCGG
CfoI 71 GCGC GCG^C
298 GCGC
EcoNI 4055 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6370 CCTTTGAGAGG
FbaI 936 TGATCA T^GATCA
6218 TGATCA
HgaI 1393 GACGC GACGC(5/10)
2129 GCGTC
HhaI 71 GCGC GCG^C
298 GCGC
HinP1I 71 GCGC G^CGC
298 GCGC
HincII 861 GTTAAC GT[CT]^[GA]AC
6974 GTTAAC
HindII 861 GTTAAC GT[CT]^[GA]AC
6974 GTTAAC
HindIII 1938 AAGCTT A^AGCTT
4784 AAGCTT
HpaI 861 GTTAAC GTT^AAC
6974 GTTAAC
KspI 135 CCGCGG CCGC^GG
405 CCGCGG
NruI 350 TCGCGA TCG^CGA
924 TCGCGA
PpuMI 1752 AGGTCCT [GA]G^G[AT]CC[CT]
1971 GGGACCC
PshBI 2709 ATTAAT AT^TAAT
4869 ATTAAT
RcaI 1239 TCATGA T^CATGA
3711 TCATGA
SacII 135 CCGCGG CCGC^GG
405 CCGCGG
SpeI 3012 ACTAGT A^CTAGT
3716 ACTAGT
XcmI 4475 CCAGCAAGCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6122 CCAAAGGAAGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BamHI BanI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrDI BsrFI
BsrGI BsrI BssKI Bst1107I BstAPI
BstF5I BstNI BstUI BstXI BstYI
BstZ17I Cac8I CelII Cfr10I Cfr13I
DdeI DpnII DraI DraII DrdI
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII