##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
AscI AseI AsnI Asp718I BbeI
BsaBI BsiEI BsiWI BspDI Bst1107I
BstZ17I ClaI DrdI EagI EclXI
Eco52I EcoRV KasI KpnI KspI
MamI MfeI MluI MunI NarI
NdeI NotI NruI PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
SacII SalI ScaI SfiI SfoI
SgrAI SpeI SspI XcmI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 4559 TCCGGA T^CCGGA
AflII 3277 CTTAAG C^TTAAG
AhdI 4543 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3502 AGCGCT AGC^GCT
ApaI 1838 GGGCCC GGGCC^C
AspEI 4543 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3226 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3801 CCTAGG C^CTAGG
BbrPI 4696 CACGTG CAC^GTG
BbvCI 3307 GCTGAGG CCTCAGC(-5/-2)
BciVI 5563 GTATCC GTATCC(6/5)
BclI 2153 TGATCA T^GATCA
BfrI 3277 CTTAAG C^TTAAG
BlnI 3801 CCTAGG C^CTAGG
BseAI 4559 TCCGGA T^CCGGA
BsmBI 565 GAGACG CGTCTC(1/5)
Bsp1407I 1749 TGTACA T^GTACA
BspEI 4559 TCCGGA T^CCGGA
BsrGI 1749 TGTACA T^GTACA
BssHII 5082 GCGCGC G^CGCGC
BstBI 6275 TTCGAA TT^CGAA
CpoI 122 CGGACCG CG^G[AT]CCG
DraIII 1630 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 4543 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3502 AGCGCT AGC^GCT
EcoRI 3616 GAATTC G^AATTC
EcoT22I 4459 ATGCAT ATGCA^T
FbaI 2153 TGATCA T^GATCA
FseI 2994 GGCCGGCC GGCCGG^CC
HpaI 5628 GTTAAC GTT^AAC
MroI 4559 TCCGGA T^CCGGA
NaeI 2995 GCCGGC GCC^GGC
NgoMIV 2995 GCCGGC G^CCGGC
NsiI 4459 ATGCAT ATGCA^T
NspV 6275 TTCGAA TT^CGAA
PflFI 3226 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4696 CACGTG CAC^GTG
PmlI 4696 CACGTG CAC^GTG
RsrII 122 CGGACCG CG^G[AT]CCG
SbfI 2958 CCTGCAGG CCTGCA^GG
SexAI 1631 ACCAGGT A^CC[AT]GGT
SfuI 6275 TTCGAA TT^CGAA
SnaBI 1884 TACGTA TAC^GTA
Sse8387I 2958 CCTGCAGG CCTGCA^GG
SspBI 1749 TGTACA T^GTACA
SwaI 6357 ATTTAAAT ATTT^AAAT
Tth111I 3226 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2187 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 2473 GACGCC G[GA]^CG[CT]C
3886 GACGCC
Alw44I 709 GTGCAC G^TGCAC
1627 GTGCAC
AocI 3762 CCTGAGG CC^T[ACGT]AGG
4153 CCTGAGG
ApaLI 709 GTGCAC G^TGCAC
1627 GTGCAC
BglI 1532 GCCAGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3100 GCCTCTGTGGC
BsaHI 2473 GACGCC G[GA]^CG[CT]C
3886 GACGCC
BsaWI 3167 ACCGGA [AT]^CCGG[AT]
4559 TCCGGA
BsmI 1203 GAATGC GAATGC(1/-1)
3303 GAATGC
BspHI 312 TCATGA T^CATGA
5574 TCATGA
BsrBI 2395 GAGCGG CCGCTC(-3/-3)
4573 GAGCGG
BsrDI 4977 CATTGC GCAATG(2/0)
2837 GCAATG
BstEII 1506 GGTGACC G^GT[ACGT]ACC
3726 GGTGACC
BstPI 1506 GGTGACC G^GT[ACGT]ACC
3726 GGTGACC
Bsu36I 3762 CCTGAGG CC^T[ACGT]AGG
4153 CCTGAGG
Eco81I 3762 CCTGAGG CC^T[ACGT]AGG
4153 CCTGAGG
EcoO65I 1506 GGTGACC G^GT[ACGT]ACC
3726 GGTGACC
Hin1I 2473 GACGCC G[GA]^CG[CT]C
3886 GACGCC
NcoI 1779 CCATGG C^CATGG
5256 CCATGG
NheI 747 GCTAGC G^CTAGC
2571 GCTAGC
PaeR7I 49 CTCGAG C^TCGAG
727 CTCGAG
RcaI 312 TCATGA T^CATGA
5574 TCATGA
SapI 1317 GAAGAGC GCTCTTC(1/4)
1056 GCTCTTC
SmaI 921 CCCGGG CCC^GGG
1835 CCCGGG
XhoI 49 CTCGAG C^TCGAG
727 CTCGAG
XmaCI 921 CCCGGG C^CCGGG
1835 CCCGGG
XmaI 921 CCCGGG C^CCGGG
1835 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII AviII BalI
BamHI BanI BanII BbsI BbvI
BcgI BcnI BfaI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI