##################################
# NO CUT ENZYMES (   49 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AgeI        
AscI        AseI        AsnI        Asp718I     BbeI        
BsaBI       BsiEI       BsiWI       BspDI       Bst1107I    
BstZ17I     ClaI        DrdI        EagI        EclXI       
Eco52I      EcoRV       KasI        KpnI        KspI        
MamI        MfeI        MluI        MunI        NarI        
NdeI        NotI        NruI        PacI        PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
SacII       SalI        ScaI        SfiI        SfoI        
SgrAI       SpeI        SspI        XcmI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccIII       4559  TCCGGA               T^CCGGA
AflII        3277  CTTAAG               C^TTAAG
AhdI         4543  GACCCGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      3502  AGCGCT               AGC^GCT
ApaI         1838  GGGCCC               GGGCC^C
AspEI        4543  GACCCGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         3226  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        3801  CCTAGG               C^CTAGG
BbrPI        4696  CACGTG               CAC^GTG
BbvCI        3307  GCTGAGG              CCTCAGC(-5/-2)
BciVI        5563  GTATCC               GTATCC(6/5)
BclI         2153  TGATCA               T^GATCA
BfrI         3277  CTTAAG               C^TTAAG
BlnI         3801  CCTAGG               C^CTAGG
BseAI        4559  TCCGGA               T^CCGGA
BsmBI         565  GAGACG               CGTCTC(1/5)
Bsp1407I     1749  TGTACA               T^GTACA
BspEI        4559  TCCGGA               T^CCGGA
BsrGI        1749  TGTACA               T^GTACA
BssHII       5082  GCGCGC               G^CGCGC
BstBI        6275  TTCGAA               TT^CGAA
CpoI          122  CGGACCG              CG^G[AT]CCG
DraIII       1630  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     4543  GACCCGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     3502  AGCGCT               AGC^GCT
EcoRI        3616  GAATTC               G^AATTC
EcoT22I      4459  ATGCAT               ATGCA^T
FbaI         2153  TGATCA               T^GATCA
FseI         2994  GGCCGGCC             GGCCGG^CC
HpaI         5628  GTTAAC               GTT^AAC
MroI         4559  TCCGGA               T^CCGGA
NaeI         2995  GCCGGC               GCC^GGC
NgoMIV       2995  GCCGGC               G^CCGGC
NsiI         4459  ATGCAT               ATGCA^T
NspV         6275  TTCGAA               TT^CGAA
PflFI        3226  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        4696  CACGTG               CAC^GTG
PmlI         4696  CACGTG               CAC^GTG
RsrII         122  CGGACCG              CG^G[AT]CCG
SbfI         2958  CCTGCAGG             CCTGCA^GG
SexAI        1631  ACCAGGT              A^CC[AT]GGT
SfuI         6275  TTCGAA               TT^CGAA
SnaBI        1884  TACGTA               TAC^GTA
Sse8387I     2958  CCTGCAGG             CCTGCA^GG
SspBI        1749  TGTACA               T^GTACA
SwaI         6357  ATTTAAAT             ATTT^AAAT
Tth111I      3226  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         2187  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcyI         2473  GACGCC               G[GA]^CG[CT]C
             3886  GACGCC              
Alw44I        709  GTGCAC               G^TGCAC
             1627  GTGCAC              
AocI         3762  CCTGAGG              CC^T[ACGT]AGG
             4153  CCTGAGG             
ApaLI         709  GTGCAC               G^TGCAC
             1627  GTGCAC              
BglI         1532  GCCAGGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3100  GCCTCTGTGGC         
BsaHI        2473  GACGCC               G[GA]^CG[CT]C
             3886  GACGCC              
BsaWI        3167  ACCGGA               [AT]^CCGG[AT]
             4559  TCCGGA              
BsmI         1203  GAATGC               GAATGC(1/-1)
             3303  GAATGC              
BspHI         312  TCATGA               T^CATGA
             5574  TCATGA              
BsrBI        2395  GAGCGG               CCGCTC(-3/-3)
             4573  GAGCGG              
BsrDI        4977  CATTGC               GCAATG(2/0)
             2837  GCAATG              
BstEII       1506  GGTGACC              G^GT[ACGT]ACC
             3726  GGTGACC             
BstPI        1506  GGTGACC              G^GT[ACGT]ACC
             3726  GGTGACC             
Bsu36I       3762  CCTGAGG              CC^T[ACGT]AGG
             4153  CCTGAGG             
Eco81I       3762  CCTGAGG              CC^T[ACGT]AGG
             4153  CCTGAGG             
EcoO65I      1506  GGTGACC              G^GT[ACGT]ACC
             3726  GGTGACC             
Hin1I        2473  GACGCC               G[GA]^CG[CT]C
             3886  GACGCC              
NcoI         1779  CCATGG               C^CATGG
             5256  CCATGG              
NheI          747  GCTAGC               G^CTAGC
             2571  GCTAGC              
PaeR7I         49  CTCGAG               C^TCGAG
              727  CTCGAG              
RcaI          312  TCATGA               T^CATGA
             5574  TCATGA              
SapI         1317  GAAGAGC              GCTCTTC(1/4)
             1056  GCTCTTC             
SmaI          921  CCCGGG               CCC^GGG
             1835  CCCGGG              
XhoI           49  CTCGAG               C^TCGAG
              727  CTCGAG              
XmaCI         921  CCCGGG               C^CCGGG
             1835  CCCGGG              
XmaI          921  CCCGGG               C^CCGGG
             1835  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  135 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        Asp700I     
AspHI       AvaI        AvaII       AviII       BalI        
BamHI       BanI        BanII       BbsI        BbvI        
BcgI        BcnI        BfaI        BglII       BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspLU11I    BspMI       BsrFI       BsrI        
BssKI       BssSI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SphI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII       XmnI