##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BcgI BglII BlnI BsiWI BspDI
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DrdI Eam1105I Eco47III EcoNI
EcoRI EcoRV EcoT22I FseI FspI
KpnI KspI MfeI MluI MunI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 575 GTCTAC GT^[AC][GT]AC
AflII 3817 CTTAAG C^TTAAG
AflIII 4587 ACATGT A^C[GA][CT]GT
Asp700I 922 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3584 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 557 GGCGCC GGCGC^C
BbrPI 1934 CACGTG CAC^GTG
BciVI 2451 GTATCC GTATCC(6/5)
BclI 4315 TGATCA T^GATCA
BfrI 3817 CTTAAG C^TTAAG
BglI 3801 GCCAGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4138 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4138 GCTCAGC GC^T[ACGT]AGC
BsaAI 1934 CACGTG [CT]AC^GT[GA]
BsaBI 563 GATTTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2754 CGGCCG CG[GA][CT]^CG
BsmBI 833 GAGACG CGTCTC(1/5)
Bsp1407I 2157 TGTACA T^GTACA
BspHI 2628 TCATGA T^CATGA
BspLU11I 4587 ACATGT A^CATGT
BsrFI 286 GCCGGC [GA]^CCGG[CT]
BsrGI 2157 TGTACA T^GTACA
BssHII 456 GCGCGC G^CGCGC
BstAPI 3369 GCAACACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 4138 GCTCAGC GC^T[ACGT]AGC
Cfr10I 286 GCCGGC [GA]^CCGG[CT]
DraIII 1109 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2754 CGGCCG C^GGCCG
EclXI 2754 CGGCCG C^GGCCG
Eco52I 2754 CGGCCG C^GGCCG
FbaI 4315 TGATCA T^GATCA
KasI 557 GGCGCC G^GCGCC
MamI 563 GATTTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 286 GCCGGC GCC^GGC
NarI 557 GGCGCC GG^CGCC
NdeI 4549 CATATG CA^TATG
NgoMIV 286 GCCGGC G^CCGGC
NheI 2344 GCTAGC G^CTAGC
PflFI 3584 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1934 CACGTG CAC^GTG
PmlI 1934 CACGTG CAC^GTG
PshAI 1309 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 3170 CTGCAG CTGCA^G
RcaI 2628 TCATGA T^CATGA
SexAI 642 ACCAGGT A^CC[AT]GGT
SfoI 557 GGCGCC GGC^GCC
SphI 2178 GCATGC GCATG^C
SspBI 2157 TGTACA T^GTACA
SspI 4461 AATATT AAT^ATT
StuI 3808 AGGCCT AGG^CCT
Tth111I 3584 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3994 TCTAGA T^CTAGA
XmnI 922 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 936 TCCGGA T^CCGGA
1677 TCCGGA
Alw44I 4070 GTGCAC G^TGCAC
4257 GTGCAC
AocI 3137 CCTGAGG CC^T[ACGT]AGG
4007 CCTGAGG
ApaLI 4070 GTGCAC G^TGCAC
4257 GTGCAC
BalI 2682 TGGCCA TGG^CCA
3697 TGGCCA
BseAI 936 TCCGGA T^CCGGA
1677 TCCGGA
BspEI 936 TCCGGA T^CCGGA
1677 TCCGGA
BsrBI 311 CCGCTC CCGCTC(-3/-3)
2666 CCGCTC
BstEII 2263 GGTCACC G^GT[ACGT]ACC
3422 GGTGACC
BstPI 2263 GGTCACC G^GT[ACGT]ACC
3422 GGTGACC
BstYI 1241 GGATCT [GA]^GATC[CT]
2907 AGATCC
Bsu36I 3137 CCTGAGG CC^T[ACGT]AGG
4007 CCTGAGG
Eco81I 3137 CCTGAGG CC^T[ACGT]AGG
4007 CCTGAGG
EcoO65I 2263 GGTCACC G^GT[ACGT]ACC
3422 GGTGACC
HaeII 557 GGCGCC [GA]GCGC^[CT]
2222 AGCGCC
HindIII 1450 AAGCTT A^AGCTT
4419 AAGCTT
HpaI 2516 GTTAAC GTT^AAC
2866 GTTAAC
MflI 1241 GGATCT [GA]^GATC[CT]
2907 AGATCC
MluNI 2682 TGGCCA TGG^CCA
3697 TGGCCA
MroI 936 TCCGGA T^CCGGA
1677 TCCGGA
MscI 2682 TGGCCA TGG^CCA
3697 TGGCCA
NcoI 876 CCATGG C^CATGG
2430 CCATGG
PflMI 1108 CCACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2329 CCACGTTCTGG
PvuII 1805 CAGCTG CAG^CTG
2737 CAGCTG
SapI 754 GCTCTTC GCTCTTC(1/4)
2668 GCTCTTC
SmlI 3817 CTTAAG C^T[CT][GA]AG
4668 CTTGAG
Van91I 1108 CCACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2329 CCACGTTCTGG
XhoII 1241 GGATCT [GA]^GATC[CT]
2907 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XmaCI XmaI