##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI AlwNI AocI AscI
Asp718I AspEI AspI BamHI BbeI
BbrPI BcgI BglI BsaBI BsaHI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BssHII Bst1107I BstEII BstPI
BstUI BstZ17I Bsu36I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I FseI HgaI
Hin1I HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NarI NheI NotI NruI
PacI PflFI PinAI PmaCI PmeI
PmlI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SpeI Sse8387I StuI
SwaI Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AclI 3584 AACGTT AA^CGTT
Aor51HI 4511 AGCGCT AGC^GCT
Asp700I 4033 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1342 CTCGAG C^[CT]CG[GA]G
AviII 3353 TGCGCA TGC^GCA
AvrII 3010 CCTAGG C^CTAGG
BbvCI 541 CCTCAGC CCTCAGC(-5/-2)
BciVI 4703 GGATAC GTATCC(6/5)
BlnI 3010 CCTAGG C^CTAGG
BsaAI 24 TACGTA [CT]AC^GT[GA]
BsoBI 1342 CTCGAG C^[CT]CG[GA]G
BsrFI 3434 GCCGGC [GA]^CCGG[CT]
BstXI 68 CCATCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 3434 GCCGGC [GA]^CCGG[CT]
Eco47III 4511 AGCGCT AGC^GCT
EcoNI 2329 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 3353 TGCGCA TGC^GCA
HindIII 4714 AAGCTT A^AGCTT
NaeI 3434 GCCGGC GCC^GGC
NgoMIV 3434 GCCGGC G^CCGGC
PaeR7I 1342 CTCGAG C^TCGAG
PflMI 1793 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1544 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3584 AACGTT AA^CGTT
SnaBI 24 TACGTA TAC^GTA
SphI 3228 GCATGC GCATG^C
SspI 1861 AATATT AAT^ATT
Van91I 1793 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1342 CTCGAG C^TCGAG
XmnI 4033 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
ApaI 53 GGGCCC GGGCC^C
4346 GGGCCC
AseI 353 ATTAAT AT^TAAT
4798 ATTAAT
AsnI 353 ATTAAT AT^TAAT
4798 ATTAAT
BalI 410 TGGCCA TGG^CCA
1983 TGGCCA
BanI 2531 GGCACC G^G[CT][GA]CC
3914 GGCACC
BclI 712 TGATCA T^GATCA
2217 TGATCA
BglII 3388 AGATCT A^GATCT
4005 AGATCT
BlpI 328 GCTGAGC GC^T[ACGT]AGC
2921 GCTGAGC
Bpu1102I 328 GCTGAGC GC^T[ACGT]AGC
2921 GCTGAGC
BspHI 823 TCATGA T^CATGA
1041 TCATGA
BssSI 1013 CACGAG CACGAG(-5/-1)
3644 CTCGTG
BstBI 2657 TTCGAA TT^CGAA
3899 TTCGAA
CelII 328 GCTGAGC GC^T[ACGT]AGC
2921 GCTGAGC
EaeI 410 TGGCCA [CT]^GGCC[GA]
1983 TGGCCA
EcoRV 335 GATATC GAT^ATC
3404 GATATC
EcoT22I 1686 ATGCAT ATGCA^T
3742 ATGCAT
FbaI 712 TGATCA T^GATCA
2217 TGATCA
HaeII 3458 AGCGCC [GA]GCGC^[CT]
4511 AGCGCT
MluNI 410 TGGCCA TGG^CCA
1983 TGGCCA
MscI 410 TGGCCA TGG^CCA
1983 TGGCCA
NdeI 2996 CATATG CA^TATG
3420 CATATG
NsiI 1686 ATGCAT ATGCA^T
3742 ATGCAT
NspV 2657 TTCGAA TT^CGAA
3899 TTCGAA
PpuMI 1485 AGGTCCT [GA]G^G[AT]CC[CT]
1542 AGGACCC
PshBI 353 ATTAAT AT^TAAT
4798 ATTAAT
RcaI 823 TCATGA T^CATGA
1041 TCATGA
SapI 1739 GAAGAGC GCTCTTC(1/4)
1018 GCTCTTC
SfuI 2657 TTCGAA TT^CGAA
3899 TTCGAA
XcmI 1236 CCAACCTCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3381 CCAAGCAAGATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI Alw44I AlwI ApaLI
ApoI AspHI AvaII BanII BbsI
BbvI BcnI BfaI BfrI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DrdI DsaI EarI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII