################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI Alw44I ApaLI AscI Asp718I AspHI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BseRI BsiHKAI BsiWI BspDI BspEI BsrBI BssHII BssSI Bst1107I BstBI BstZ17I CelII ClaI CpoI DraII DraIII EagI EclXI Eco52I EcoO109I EcoRV EcoT22I FseI FspI Hin1I HpaI KasI KpnI KspI MluI MroI NaeI NarI NciI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RsrII SacI SacII SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I StuI SwaI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccII 176 CGCG CG^CG AhdI 4397 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 3212 CCTCAGG CC^T[ACGT]AGG ApaI 532 GGGCCC GGGCC^C Asp700I 2656 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 4397 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 63 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 4130 TGGCCA TGG^CCA BbsI 2148 GTCTTC GAAGAC(2/6) BpuAI 2148 GTCTTC GAAGAC(2/6) BsiEI 41 CGACCG CG[GA][CT]^CG BsmBI 2723 CGTCTC CGTCTC(1/5) BsrFI 43 ACCGGC [GA]^CCGG[CT] BstEII 3061 GGTAACC G^GT[ACGT]ACC BstPI 3061 GGTAACC G^GT[ACGT]ACC BstUI 176 CGCG CG^CG Bsu36I 3212 CCTCAGG CC^T[ACGT]AGG Cfr10I 43 ACCGGC [GA]^CCGG[CT] DrdI 1160 GACTAGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 4130 TGGCCA [CT]^GGCC[GA] Eam1105I 4397 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 3212 CCTCAGG CC^T[ACGT]AGG EcoO65I 3061 GGTAACC G^GT[ACGT]ACC EcoRI 3749 GAATTC G^AATTC HgaI 172 GCGTC GACGC(5/10) MaeII 1899 ACGT A^CGT MfeI 598 CAATTG C^AATTG MluNI 4130 TGGCCA TGG^CCA MscI 4130 TGGCCA TGG^CCA MunI 598 CAATTG C^AATTG MvnI 176 CGCG CG^CG NheI 1266 GCTAGC G^CTAGC PflFI 63 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 3114 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SalI 718 GTCGAC G^TCGAC ScaI 2113 AGTACT AGT^ACT SpeI 3878 ACTAGT A^CTAGT SspI 4942 AATATT AAT^ATT TaqI 719 TCGA T^CGA Tth111I 63 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 719 TCGA T^CGA XcmI 397 CCATCATCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 2656 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 718 GTCGAC GT^[AC][GT]AC 4381 GTAGAC Aor51HI 1072 AGCGCT AGC^GCT 2175 AGCGCT AvaI 2103 CTCGGG C^[CT]CG[GA]G 2393 CCCGAG AvaII 219 GGACC G^G[AT]CC 3394 GGACC BglII 1373 AGATCT A^GATCT 2346 AGATCT BsaBI 906 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2341 GATCAAGATC BsaI 32 GGTCTC GGTCTC(1/5) 68 GGTCTC BsoBI 2103 CTCGGG C^[CT]CG[GA]G 2393 CCCGAG BspHI 278 TCATGA T^CATGA 914 TCATGA BstXI 491 CCATAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 797 CCAATACTGTGG DsaI 222 CCATGG C^C[GA][CT]GG 3483 CCATGG Eco47III 1072 AGCGCT AGC^GCT 2175 AGCGCT FauI 140 CCCGC CCCGC(4/6) 1586 GCGGG HaeII 1072 AGCGCT [GA]GCGC^[CT] 2175 AGCGCT HincII 718 GTCGAC GT[CT]^[GA]AC 3554 GTTGAC HindII 718 GTCGAC GT[CT]^[GA]AC 3554 GTTGAC HindIII 187 AAGCTT A^AGCTT 1526 AAGCTT MamI 906 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2341 GATCAAGATC NcoI 222 CCATGG C^CATGG 3483 CCATGG NdeI 865 CATATG CA^TATG 2272 CATATG RcaI 278 TCATGA T^CATGA 914 TCATGA XbaI 346 TCTAGA T^CTAGA 3572 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI BanI BanII BbvCI BbvI BclI BfaI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspLU11I BspMI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoNI EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII