##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I ApaLI AscI
Asp718I AspHI AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BseRI
BsiHKAI BsiWI BspDI BspEI BsrBI
BssHII BssSI Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraII DraIII
EagI EclXI Eco52I EcoO109I EcoRV
EcoT22I FseI FspI Hin1I HpaI
KasI KpnI KspI MluI MroI
NaeI NarI NciI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI RsrII SacI
SacII SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I StuI SwaI Van91I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 176 CGCG CG^CG
AhdI 4397 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3212 CCTCAGG CC^T[ACGT]AGG
ApaI 532 GGGCCC GGGCC^C
Asp700I 2656 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4397 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 63 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 4130 TGGCCA TGG^CCA
BbsI 2148 GTCTTC GAAGAC(2/6)
BpuAI 2148 GTCTTC GAAGAC(2/6)
BsiEI 41 CGACCG CG[GA][CT]^CG
BsmBI 2723 CGTCTC CGTCTC(1/5)
BsrFI 43 ACCGGC [GA]^CCGG[CT]
BstEII 3061 GGTAACC G^GT[ACGT]ACC
BstPI 3061 GGTAACC G^GT[ACGT]ACC
BstUI 176 CGCG CG^CG
Bsu36I 3212 CCTCAGG CC^T[ACGT]AGG
Cfr10I 43 ACCGGC [GA]^CCGG[CT]
DrdI 1160 GACTAGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 4130 TGGCCA [CT]^GGCC[GA]
Eam1105I 4397 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3212 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3061 GGTAACC G^GT[ACGT]ACC
EcoRI 3749 GAATTC G^AATTC
HgaI 172 GCGTC GACGC(5/10)
MaeII 1899 ACGT A^CGT
MfeI 598 CAATTG C^AATTG
MluNI 4130 TGGCCA TGG^CCA
MscI 4130 TGGCCA TGG^CCA
MunI 598 CAATTG C^AATTG
MvnI 176 CGCG CG^CG
NheI 1266 GCTAGC G^CTAGC
PflFI 63 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3114 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 718 GTCGAC G^TCGAC
ScaI 2113 AGTACT AGT^ACT
SpeI 3878 ACTAGT A^CTAGT
SspI 4942 AATATT AAT^ATT
TaqI 719 TCGA T^CGA
Tth111I 63 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 719 TCGA T^CGA
XcmI 397 CCATCATCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2656 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 718 GTCGAC GT^[AC][GT]AC
4381 GTAGAC
Aor51HI 1072 AGCGCT AGC^GCT
2175 AGCGCT
AvaI 2103 CTCGGG C^[CT]CG[GA]G
2393 CCCGAG
AvaII 219 GGACC G^G[AT]CC
3394 GGACC
BglII 1373 AGATCT A^GATCT
2346 AGATCT
BsaBI 906 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2341 GATCAAGATC
BsaI 32 GGTCTC GGTCTC(1/5)
68 GGTCTC
BsoBI 2103 CTCGGG C^[CT]CG[GA]G
2393 CCCGAG
BspHI 278 TCATGA T^CATGA
914 TCATGA
BstXI 491 CCATAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
797 CCAATACTGTGG
DsaI 222 CCATGG C^C[GA][CT]GG
3483 CCATGG
Eco47III 1072 AGCGCT AGC^GCT
2175 AGCGCT
FauI 140 CCCGC CCCGC(4/6)
1586 GCGGG
HaeII 1072 AGCGCT [GA]GCGC^[CT]
2175 AGCGCT
HincII 718 GTCGAC GT[CT]^[GA]AC
3554 GTTGAC
HindII 718 GTCGAC GT[CT]^[GA]AC
3554 GTTGAC
HindIII 187 AAGCTT A^AGCTT
1526 AAGCTT
MamI 906 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2341 GATCAAGATC
NcoI 222 CCATGG C^CATGG
3483 CCATGG
NdeI 865 CATATG CA^TATG
2272 CATATG
RcaI 278 TCATGA T^CATGA
914 TCATGA
XbaI 346 TCTAGA T^CTAGA
3572 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
BanI BanII BbvCI BbvI BclI
BfaI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspLU11I BspMI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI EcoNI
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII