##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Aor51HI AscI Asp700I Asp718I AspEI
AviII AvrII BbvCI BciVI BlnI
BlpI Bpu1102I BsiWI BsmBI BspDI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I CelII ClaI CpoI Eam1105I
Eco47III EcoNI EcoO65I FseI FspI
HpaI KpnI MluI NcoI NdeI
NheI NruI PacI PaeR7I PflMI
PinAI PmeI Psp1406I PvuI PvuII
RsrII SbfI SfiI SgrAI SnaBI
SpeI Sse8387I StuI SwaI Van91I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 106 TCCGGA T^CCGGA
AflII 4481 CTTAAG C^TTAAG
Alw44I 2454 GTGCAC G^TGCAC
AlwNI 1663 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2454 GTGCAC G^TGCAC
AspI 165 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 157 GGCGCC GGCGC^C
BbrPI 2009 CACGTG CAC^GTG
BcgI 2735 CGACTGTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4481 CTTAAG C^TTAAG
BglII 2955 AGATCT A^GATCT
BsaBI 4396 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 106 TCCGGA [AT]^CCGG[AT]
BseAI 106 TCCGGA T^CCGGA
BsgI 627 CTGCAC GTGCAG(16/14)
BspEI 106 TCCGGA T^CCGGA
BspMI 3906 ACCTGC ACCTGC(4/8)
BsrBI 654 CCGCTC CCGCTC(-3/-3)
BstBI 915 TTCGAA TT^CGAA
DraIII 1819 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2624 GACCATCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 2408 GATATC GAT^ATC
EcoT22I 3671 ATGCAT ATGCA^T
KasI 157 GGCGCC G^GCGCC
KspI 521 CCGCGG CCGC^GG
MamI 4396 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3840 CAATTG C^AATTG
MroI 106 TCCGGA T^CCGGA
MunI 3840 CAATTG C^AATTG
NarI 157 GGCGCC GG^CGCC
NotI 553 GCGGCCGC GC^GGCCGC
NsiI 3671 ATGCAT ATGCA^T
NspV 915 TTCGAA TT^CGAA
PflFI 165 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2009 CACGTG CAC^GTG
PmlI 2009 CACGTG CAC^GTG
PpuMI 808 AGGACCT [GA]G^G[AT]CC[CT]
SacI 1480 GAGCTC GAGCT^C
SacII 521 CCGCGG CCGC^GG
SalI 2733 GTCGAC G^TCGAC
SapI 656 GCTCTTC GCTCTTC(1/4)
ScaI 1392 AGTACT AGT^ACT
SexAI 4191 ACCAGGT A^CC[AT]GGT
SfoI 157 GGCGCC GGC^GCC
SfuI 915 TTCGAA TT^CGAA
Tth111I 165 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3052 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BclI 4395 TGATCA T^GATCA
4805 TGATCA
BsaAI 2009 CACGTG [CT]AC^GT[GA]
4933 TACGTG
BsmI 3601 GCATTC GAATGC(1/-1)
3673 GCATTC
Bsp1407I 3925 TGTACA T^GTACA
4080 TGTACA
BspHI 4224 TCATGA T^CATGA
4392 TCATGA
BsrGI 3925 TGTACA T^GTACA
4080 TGTACA
BstAPI 2082 GCAGGCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3901 GCATGACCTGC
DsaI 403 CCACGG C^C[GA][CT]GG
521 CCGCGG
EcoRI 1185 GAATTC G^AATTC
3683 GAATTC
FbaI 4395 TGATCA T^GATCA
4805 TGATCA
HincII 2733 GTCGAC GT[CT]^[GA]AC
4352 GTCAAC
HindII 2733 GTCGAC GT[CT]^[GA]AC
4352 GTCAAC
NaeI 41 GCCGGC GCC^GGC
48 GCCGGC
NgoMIV 41 GCCGGC G^CCGGC
48 GCCGGC
RcaI 4224 TCATGA T^CATGA
4392 TCATGA
SmaI 474 CCCGGG CCC^GGG
581 CCCGGG
SphI 1609 GCATGC GCATG^C
5092 GCATGC
SspBI 3925 TGTACA T^GTACA
4080 TGTACA
XcmI 1561 CCATGACTACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4192 CCAGGTTGCACTTGG
XmaCI 474 CCCGGG C^CCGGG
581 CCCGGG
XmaI 474 CCCGGG C^CCGGG
581 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AocI
ApaI ApoI AseI AsnI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
EaeI EagI EarI EclXI Eco52I
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PshAI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII