##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Alw44I ApaLI AscI AspEI
AspI AviII BbeI BglI BglII
BsaHI BseAI BsgI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BsrFI
BssHII Bst1107I BstBI BstZ17I Cfr10I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco52I EcoRI FseI FspI
HgaI Hin1I HindIII HpaI KasI
KspI MluI MroI NaeI NarI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 3836 GGTACC G^GTACC
AflII 4705 CTTAAG C^TTAAG
AflIII 2165 ACATGT A^C[GA][CT]GT
Aor51HI 348 AGCGCT AGC^GCT
AseI 2310 ATTAAT AT^TAAT
AsnI 2310 ATTAAT AT^TAAT
Asp700I 59 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3836 GGTACC G^GTACC
AvaI 3017 CTCGGG C^[CT]CG[GA]G
BbrPI 1189 CACGTG CAC^GTG
BcgI 1536 CGAAGCCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1423 GTATCC GTATCC(6/5)
BcnI 1923 CCGGG CC^[GC]GG
BfrI 4705 CTTAAG C^TTAAG
BsaBI 1418 GATTAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3465 TCCGGT [AT]^CCGG[AT]
BsoBI 3017 CTCGGG C^[CT]CG[GA]G
BspLU11I 2165 ACATGT A^CATGT
BsrBI 5123 CCGCTC CCGCTC(-3/-3)
BssSI 272 CACGAG CACGAG(-5/-1)
DrdI 4711 GACCCCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3799 CCATGG C^C[GA][CT]GG
Eco47III 348 AGCGCT AGC^GCT
EcoRV 446 GATATC GAT^ATC
FauI 4606 GCGGG CCCGC(4/6)
HaeII 348 AGCGCT [GA]GCGC^[CT]
HincII 3936 GTCAAC GT[CT]^[GA]AC
HindII 3936 GTCAAC GT[CT]^[GA]AC
KpnI 3836 GGTACC GGTAC^C
MamI 1418 GATTAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1041 CAATTG C^AATTG
MunI 1041 CAATTG C^AATTG
NciI 1923 CCGGG CC^[GC]GG
NcoI 3799 CCATGG C^CATGG
NdeI 3092 CATATG CA^TATG
NheI 1486 GCTAGC G^CTAGC
PmaCI 1189 CACGTG CAC^GTG
PmlI 1189 CACGTG CAC^GTG
PshAI 2730 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2310 ATTAAT AT^TAAT
SphI 4598 GCATGC GCATG^C
SspI 2313 AATATT AAT^ATT
TaqI 3906 TCGA T^CGA
TthHB8I 3906 TCGA T^CGA
XmnI 59 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 2925 GTCTAC GT^[AC][GT]AC
3768 GTAGAC
AccII 1901 CGCG CG^CG
1978 CGCG
ApaI 197 GGGCCC GGGCC^C
4000 GGGCCC
BamHI 1630 GGATCC G^GATCC
4771 GGATCC
BbvCI 235 CCTCAGC CCTCAGC(-5/-2)
1945 CCTCAGC
BclI 655 TGATCA T^GATCA
1050 TGATCA
BlpI 1501 GCTAAGC GC^T[ACGT]AGC
1702 GCTGAGC
Bpu1102I 1501 GCTAAGC GC^T[ACGT]AGC
1702 GCTGAGC
BsaAI 698 TACGTG [CT]AC^GT[GA]
1189 CACGTG
BsmI 3064 GAATGC GAATGC(1/-1)
2460 GCATTC
Bsp1407I 1433 TGTACA T^GTACA
3009 TGTACA
BspMI 3620 ACCTGC ACCTGC(4/8)
4903 ACCTGC
BsrGI 1433 TGTACA T^GTACA
3009 TGTACA
BstEII 485 GGTGACC G^GT[ACGT]ACC
3642 GGTGACC
BstPI 485 GGTGACC G^GT[ACGT]ACC
3642 GGTGACC
BstUI 1901 CGCG CG^CG
1978 CGCG
CelII 1501 GCTAAGC GC^T[ACGT]AGC
1702 GCTGAGC
DraI 477 TTTAAA TTT^AAA
3086 TTTAAA
EcoNI 3275 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5029 CCTTCTCCAGG
EcoO65I 485 GGTGACC G^GT[ACGT]ACC
3642 GGTGACC
FbaI 655 TGATCA T^GATCA
1050 TGATCA
HapII 1923 CCGG C^CGG
3466 CCGG
HpaII 1923 CCGG C^CGG
3466 CCGG
MspI 1923 CCGG C^CGG
3466 CCGG
MvnI 1901 CGCG CG^CG
1978 CGCG
SacI 583 GAGCTC GAGCT^C
4340 GAGCTC
ScaI 2530 AGTACT AGT^ACT
3483 AGTACT
SspBI 1433 TGTACA T^GTACA
3009 TGTACA
XbaI 1661 TCTAGA T^CTAGA
3161 TCTAGA
XcmI 328 CCAGAAGATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3529 CCACTGATCTACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
AvrII BalI BanI BanII BbsI
BbvI BfaI BlnI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII