##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI BamHI BbrPI BfrI
BglII BsaWI BseAI BsiEI BsiHKAI
BsiWI BspDI BspEI BspMI BsrBI
BssHII BstBI BstEII BstPI BstUI
ClaI CpoI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FseI HgaI HpaI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SpeI SphI Sse8387I XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 1300 GGCGCC G[GA]^CG[CT]C
AflIII 3846 ACATGT A^C[GA][CT]GT
AocI 2102 CCTCAGG CC^T[ACGT]AGG
AspI 5179 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 329 CCTAGG C^CTAGG
BbeI 1300 GGCGCC GGCGC^C
BciVI 3158 GGATAC GTATCC(6/5)
BclI 4638 TGATCA T^GATCA
BlnI 329 CCTAGG C^CTAGG
BlpI 3012 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3012 GCTGAGC GC^T[ACGT]AGC
BsaAI 1539 TACGTA [CT]AC^GT[GA]
BsaHI 1300 GGCGCC G[GA]^CG[CT]C
BseRI 1324 CTCCTC GAGGAG(10/8)
BspLU11I 3846 ACATGT A^CATGT
BsrFI 3424 ACCGGC [GA]^CCGG[CT]
BssSI 690 CTCGTG CACGAG(-5/-1)
Bsu36I 2102 CCTCAGG CC^T[ACGT]AGG
CelII 3012 GCTGAGC GC^T[ACGT]AGC
Cfr10I 3424 ACCGGC [GA]^CCGG[CT]
DraIII 3799 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2102 CCTCAGG CC^T[ACGT]AGG
EcoNI 1326 CCTCCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 4638 TGATCA T^GATCA
Hin1I 1300 GGCGCC G[GA]^CG[CT]C
HincII 4421 GTTGAC GT[CT]^[GA]AC
HindII 4421 GTTGAC GT[CT]^[GA]AC
KasI 1300 GGCGCC G^GCGCC
MspA1I 782 CAGCTG C[AC]G^C[GT]G
NarI 1300 GGCGCC GG^CGCC
PaeR7I 635 CTCGAG C^TCGAG
PflFI 5179 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 4404 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 782 CAGCTG CAG^CTG
SfoI 1300 GGCGCC GGC^GCC
SnaBI 1539 TACGTA TAC^GTA
StuI 874 AGGCCT AGG^CCT
SwaI 2277 ATTTAAAT ATTT^AAAT
Tth111I 5179 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3748 TCTAGA T^CTAGA
XhoI 635 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 2555 GTATAC GT^[AC][GT]AC
3066 GTATAC
AviII 866 TGCGCA TGC^GCA
1387 TGCGCA
BanI 1300 GGCGCC G^G[CT][GA]CC
3703 GGCACC
BbvCI 1177 CCTCAGC CCTCAGC(-5/-2)
1986 CCTCAGC
BcgI 40 CGAGGCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1166 CGATCCTCCTGC
BcnI 2031 CCCGG CC^[GC]GG
3343 CCGGG
BsmI 922 GAATGC GAATGC(1/-1)
4719 GCATTC
Bsp1407I 2285 TGTACA T^GTACA
2711 TGTACA
BsrGI 2285 TGTACA T^GTACA
2711 TGTACA
Bst1107I 2555 GTATAC GTA^TAC
3066 GTATAC
BstYI 1552 AGATCC [GA]^GATC[CT]
4297 GGATCT
BstZ17I 2555 GTATAC GTA^TAC
3066 GTATAC
DraII 244 AGGCCCT [GA]G^G[ACGT]CC[CT]
4404 AGGACCT
EcoO109I 244 AGGCCCT [GA]G^G[ACGT]CC[CT]
4404 AGGACCT
EcoRI 2728 GAATTC G^AATTC
3140 GAATTC
FspI 866 TGCGCA TGC^GCA
1387 TGCGCA
HaeII 31 GGCGCT [GA]GCGC^[CT]
1300 GGCGCC
HindIII 1732 AAGCTT A^AGCTT
4477 AAGCTT
MflI 1552 AGATCC [GA]^GATC[CT]
4297 GGATCT
NciI 2031 CCCGG CC^[GC]GG
3343 CCGGG
PflMI 2603 CCATTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3688 CCATTGACTGG
SspBI 2285 TGTACA T^GTACA
2711 TGTACA
Van91I 2603 CCATTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3688 CCATTGACTGG
XhoII 1552 AGATCC [GA]^GATC[CT]
4297 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I AvaI
AvaII BalI BanII BbsI BbvI
BfaI BglI BmyI BpmI BpuAI
BsaBI BsaI BsaJI BsgI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspHI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EaeI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NdeI NdeII NlaIII NlaIV NspI
PleI PshBI PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI