##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AscI Asp718I AspI AviII BalI
BbrPI BbsI BfrI BpuAI BsaBI
BsaI BsgI BsiWI BspDI BspMI
BstEII BstPI ClaI CpoI DrdI
EcoNI EcoO65I FseI FspI HpaI
KpnI KspI MamI MluNI MscI
NcoI NheI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SbfI SexAI
SfiI SmlI SpeI Sse8387I SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 25 TCCGGA T^CCGGA
AhdI 2017 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3374 GTGCAC G^TGCAC
ApaLI 3374 GTGCAC G^TGCAC
AspEI 2017 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2195 GGCGCC GGCGC^C
BbvCI 3785 GCTGAGG CCTCAGC(-5/-2)
BciVI 5675 GGATAC GTATCC(6/5)
BglII 4926 AGATCT A^GATCT
BsaWI 25 TCCGGA [AT]^CCGG[AT]
BseAI 25 TCCGGA T^CCGGA
BspEI 25 TCCGGA T^CCGGA
BspLU11I 1117 ACATGT A^CATGT
BssHII 282 GCGCGC G^CGCGC
Bst1107I 3583 GTATAC GTA^TAC
BstBI 3566 TTCGAA TT^CGAA
BstZ17I 3583 GTATAC GTA^TAC
Eam1105I 2017 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 3718 GTCAAC GT[CT]^[GA]AC
HindII 3718 GTCAAC GT[CT]^[GA]AC
KasI 2195 GGCGCC G^GCGCC
MfeI 5662 CAATTG C^AATTG
MluI 5367 ACGCGT A^CGCGT
MroI 25 TCCGGA T^CCGGA
MslI 5068 CATTCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 5662 CAATTG C^AATTG
NarI 2195 GGCGCC GG^CGCC
NdeI 2478 CATATG CA^TATG
NotI 305 GCGGCCGC GC^GGCCGC
NspV 3566 TTCGAA TT^CGAA
ScaI 5544 AGTACT AGT^ACT
SfoI 2195 GGCGCC GGC^GCC
SfuI 3566 TTCGAA TT^CGAA
SgrAI 310 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 204 CCCGGG CCC^GGG
SnaBI 2925 TACGTA TAC^GTA
SphI 2805 GCATGC GCATG^C
XcmI 1849 CCATTTTAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 204 CCCGGG C^CCGGG
XmaI 204 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 1790 GACGCC G[GA]^CG[CT]C
2195 GGCGCC
AocI 393 CCTGAGG CC^T[ACGT]AGG
3929 CCTAAGG
Aor51HI 1864 AGCGCT AGC^GCT
3704 AGCGCT
AseI 2378 ATTAAT AT^TAAT
3650 ATTAAT
AsnI 2378 ATTAAT AT^TAAT
3650 ATTAAT
Asp700I 1412 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1649 GAAACCATTC
AspHI 2009 GAGCAC G[AT]GC[AT]^C
3374 GTGCAC
AvrII 758 CCTAGG C^CTAGG
5529 CCTAGG
BcgI 3568 CGAAAATATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
943 GCACGTTTTTCG
BclI 2508 TGATCA T^GATCA
5713 TGATCA
BlnI 758 CCTAGG C^CTAGG
5529 CCTAGG
BsaAI 2925 TACGTA [CT]AC^GT[GA]
3683 TACGTG
BsaHI 1790 GACGCC G[GA]^CG[CT]C
2195 GGCGCC
BseRI 634 CTCCTC GAGGAG(10/8)
2845 CTCCTC
BsiHKAI 2009 GAGCAC G[AT]GC[AT]^C
3374 GTGCAC
BsmBI 5767 GAGACG CGTCTC(1/5)
5776 GAGACG
BspHI 3327 TCATGA T^CATGA
3457 TCATGA
BsrBI 2203 CCGCTC CCGCTC(-3/-3)
995 GAGCGG
BsrDI 1139 CATTGC GCAATG(2/0)
2319 GCAATG
BssSI 4450 CTCGTG CACGAG(-5/-1)
5807 CTCGTG
BstAPI 61 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1362 GCAGCTTTTGC
BstXI 1915 CCATGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1944 CCAGAGATTTGG
Bsu36I 393 CCTGAGG CC^T[ACGT]AGG
3929 CCTAAGG
DsaI 169 CCACGG C^C[GA][CT]GG
269 CCGTGG
EagI 306 CGGCCG C^GGCCG
406 CGGCCG
EclXI 306 CGGCCG C^GGCCG
406 CGGCCG
Eco47III 1864 AGCGCT AGC^GCT
3704 AGCGCT
Eco52I 306 CGGCCG C^GGCCG
406 CGGCCG
Eco81I 393 CCTGAGG CC^T[ACGT]AGG
3929 CCTAAGG
EcoRI 3054 GAATTC G^AATTC
5619 GAATTC
EcoRV 1458 GATATC GAT^ATC
4798 GATATC
EcoT22I 2807 ATGCAT ATGCA^T
3426 ATGCAT
FbaI 2508 TGATCA T^GATCA
5713 TGATCA
Hin1I 1790 GACGCC G[GA]^CG[CT]C
2195 GGCGCC
NaeI 311 GCCGGC GCC^GGC
404 GCCGGC
NgoMIV 311 GCCGGC G^CCGGC
404 GCCGGC
NsiI 2807 ATGCAT ATGCA^T
3426 ATGCAT
PshBI 2378 ATTAAT AT^TAAT
3650 ATTAAT
RcaI 3327 TCATGA T^CATGA
3457 TCATGA
StuI 4235 AGGCCT AGG^CCT
4757 AGGCCT
XmnI 1412 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1649 GAAACCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AvaI AvaII BamHI BanI
BanII BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaJI
BsiEI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII