################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AscI Asp718I AspI AviII BalI BbrPI BbsI BfrI BpuAI BsaBI BsaI BsgI BsiWI BspDI BspMI BstEII BstPI ClaI CpoI DrdI EcoNI EcoO65I FseI FspI HpaI KpnI KspI MamI MluNI MscI NcoI NheI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI SexAI SfiI SmlI SpeI Sse8387I SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccIII 25 TCCGGA T^CCGGA AhdI 2017 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3374 GTGCAC G^TGCAC ApaLI 3374 GTGCAC G^TGCAC AspEI 2017 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 2195 GGCGCC GGCGC^C BbvCI 3785 GCTGAGG CCTCAGC(-5/-2) BciVI 5675 GGATAC GTATCC(6/5) BglII 4926 AGATCT A^GATCT BsaWI 25 TCCGGA [AT]^CCGG[AT] BseAI 25 TCCGGA T^CCGGA BspEI 25 TCCGGA T^CCGGA BspLU11I 1117 ACATGT A^CATGT BssHII 282 GCGCGC G^CGCGC Bst1107I 3583 GTATAC GTA^TAC BstBI 3566 TTCGAA TT^CGAA BstZ17I 3583 GTATAC GTA^TAC Eam1105I 2017 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HincII 3718 GTCAAC GT[CT]^[GA]AC HindII 3718 GTCAAC GT[CT]^[GA]AC KasI 2195 GGCGCC G^GCGCC MfeI 5662 CAATTG C^AATTG MluI 5367 ACGCGT A^CGCGT MroI 25 TCCGGA T^CCGGA MslI 5068 CATTCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 5662 CAATTG C^AATTG NarI 2195 GGCGCC GG^CGCC NdeI 2478 CATATG CA^TATG NotI 305 GCGGCCGC GC^GGCCGC NspV 3566 TTCGAA TT^CGAA ScaI 5544 AGTACT AGT^ACT SfoI 2195 GGCGCC GGC^GCC SfuI 3566 TTCGAA TT^CGAA SgrAI 310 CGCCGGCG C[GA]^CCGG[CT]G SmaI 204 CCCGGG CCC^GGG SnaBI 2925 TACGTA TAC^GTA SphI 2805 GCATGC GCATG^C XcmI 1849 CCATTTTAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 204 CCCGGG C^CCGGG XmaI 204 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 41 enzymes) # ################################### AcyI 1790 GACGCC G[GA]^CG[CT]C 2195 GGCGCC AocI 393 CCTGAGG CC^T[ACGT]AGG 3929 CCTAAGG Aor51HI 1864 AGCGCT AGC^GCT 3704 AGCGCT AseI 2378 ATTAAT AT^TAAT 3650 ATTAAT AsnI 2378 ATTAAT AT^TAAT 3650 ATTAAT Asp700I 1412 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1649 GAAACCATTC AspHI 2009 GAGCAC G[AT]GC[AT]^C 3374 GTGCAC AvrII 758 CCTAGG C^CTAGG 5529 CCTAGG BcgI 3568 CGAAAATATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 943 GCACGTTTTTCG BclI 2508 TGATCA T^GATCA 5713 TGATCA BlnI 758 CCTAGG C^CTAGG 5529 CCTAGG BsaAI 2925 TACGTA [CT]AC^GT[GA] 3683 TACGTG BsaHI 1790 GACGCC G[GA]^CG[CT]C 2195 GGCGCC BseRI 634 CTCCTC GAGGAG(10/8) 2845 CTCCTC BsiHKAI 2009 GAGCAC G[AT]GC[AT]^C 3374 GTGCAC BsmBI 5767 GAGACG CGTCTC(1/5) 5776 GAGACG BspHI 3327 TCATGA T^CATGA 3457 TCATGA BsrBI 2203 CCGCTC CCGCTC(-3/-3) 995 GAGCGG BsrDI 1139 CATTGC GCAATG(2/0) 2319 GCAATG BssSI 4450 CTCGTG CACGAG(-5/-1) 5807 CTCGTG BstAPI 61 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1362 GCAGCTTTTGC BstXI 1915 CCATGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1944 CCAGAGATTTGG Bsu36I 393 CCTGAGG CC^T[ACGT]AGG 3929 CCTAAGG DsaI 169 CCACGG C^C[GA][CT]GG 269 CCGTGG EagI 306 CGGCCG C^GGCCG 406 CGGCCG EclXI 306 CGGCCG C^GGCCG 406 CGGCCG Eco47III 1864 AGCGCT AGC^GCT 3704 AGCGCT Eco52I 306 CGGCCG C^GGCCG 406 CGGCCG Eco81I 393 CCTGAGG CC^T[ACGT]AGG 3929 CCTAAGG EcoRI 3054 GAATTC G^AATTC 5619 GAATTC EcoRV 1458 GATATC GAT^ATC 4798 GATATC EcoT22I 2807 ATGCAT ATGCA^T 3426 ATGCAT FbaI 2508 TGATCA T^GATCA 5713 TGATCA Hin1I 1790 GACGCC G[GA]^CG[CT]C 2195 GGCGCC NaeI 311 GCCGGC GCC^GGC 404 GCCGGC NgoMIV 311 GCCGGC G^CCGGC 404 GCCGGC NsiI 2807 ATGCAT ATGCA^T 3426 ATGCAT PshBI 2378 ATTAAT AT^TAAT 3650 ATTAAT RcaI 3327 TCATGA T^CATGA 3457 TCATGA StuI 4235 AGGCCT AGG^CCT 4757 AGGCCT XmnI 1412 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1649 GAAACCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApaI ApoI AvaI AvaII BamHI BanI BanII BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaJI BsiEI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII