################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp718I AspEI AvrII BbeI BbrPI BfrI BlnI BsaAI BseAI BsiEI BsiWI Bsp1407I BspEI BspHI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CpoI DraI DraIII EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI HindIII HpaI KasI KpnI MfeI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SfoI SfuI SgrAI SmaI SnaBI SphI SspBI SspI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 23 enzymes) # ################################### AatII 2606 GACGTC GACGT^C AccI 4520 GTAGAC GT^[AC][GT]AC ApaI 3597 GGGCCC GGGCC^C Asp700I 1036 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 4759 GGTGCC G^G[CT][GA]CC BciVI 1278 GTATCC GTATCC(6/5) BsaBI 3790 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2694 ACCGGA [AT]^CCGG[AT] BspDI 1778 ATCGAT AT^CGAT BspLU11I 393 ACATGT A^CATGT BstXI 3813 CCAACCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 1778 ATCGAT AT^CGAT KspI 2484 CCGCGG CCGC^GG MamI 3790 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 1274 ACGCGT A^CGCGT NcoI 498 CCATGG C^CATGG PaeR7I 3074 CTCGAG C^TCGAG SacII 2484 CCGCGG CCGC^GG ScaI 279 AGTACT AGT^ACT SexAI 4515 ACCTGGT A^CC[AT]GGT SpeI 4043 ACTAGT A^CTAGT XhoI 3074 CTCGAG C^TCGAG XmnI 1036 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 2606 GACGTC G[GA]^CG[CT]C 3050 GACGCC AflIII 393 ACATGT A^C[GA][CT]GT 1274 ACGCGT AocI 1109 CCTGAGG CC^T[ACGT]AGG 3751 CCTGAGG Aor51HI 1626 AGCGCT AGC^GCT 3216 AGCGCT AviII 2049 TGCGCA TGC^GCA 2493 TGCGCA BamHI 712 GGATCC G^GATCC 2024 GGATCC BfaI 1505 CTAG C^TAG 4044 CTAG BglII 315 AGATCT A^GATCT 3441 AGATCT BsaHI 2606 GACGTC G[GA]^CG[CT]C 3050 GACGCC BsmBI 2966 GAGACG CGTCTC(1/5) 3044 GAGACG BsrDI 138 GCAATG GCAATG(2/0) 1002 GCAATG Bsu36I 1109 CCTGAGG CC^T[ACGT]AGG 3751 CCTGAGG DrdI 3890 GACGATGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4000 GACAGAGCGGTC Eco47III 1626 AGCGCT AGC^GCT 3216 AGCGCT Eco81I 1109 CCTGAGG CC^T[ACGT]AGG 3751 CCTGAGG FspI 2049 TGCGCA TGC^GCA 2493 TGCGCA Hin1I 2606 GACGTC G[GA]^CG[CT]C 3050 GACGCC MaeI 1505 CTAG C^TAG 4044 CTAG NaeI 665 GCCGGC GCC^GGC 2375 GCCGGC NgoMIV 665 GCCGGC G^CCGGC 2375 GCCGGC NspI 393 ACATGT [GA]CATG^[CT] 531 GCATGT PflMI 2845 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3487 CCATGAGCTGG SbfI 466 CCTGCAGG CCTGCA^GG 3430 CCTGCAGG Sse8387I 466 CCTGCAGG CCTGCA^GG 3430 CCTGCAGG TfiI 1654 GATTC G^A[AT]TC 3880 GATTC Tsp509I 1394 AATT ^AATT 4823 AATT Van91I 2845 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3487 CCATGAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AspI AvaI AvaII BalI BanII BbsI BbvCI BbvI BcgI BclI BcnI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflFI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SmlI StuI StyI TaqI Tru9I TseI Tsp45I TspRI Tth111I TthHB8I XcmI XhoII