##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI Alw44I ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AvrII BbeI BbrPI BfrI BlnI
BsaAI BseAI BsiEI BsiWI Bsp1407I
BspEI BspHI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CpoI
DraI DraIII EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI HindIII HpaI KasI KpnI
MfeI MroI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SfoI SfuI SgrAI
SmaI SnaBI SphI SspBI SspI
SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 23 enzymes) #
###################################
AatII 2606 GACGTC GACGT^C
AccI 4520 GTAGAC GT^[AC][GT]AC
ApaI 3597 GGGCCC GGGCC^C
Asp700I 1036 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 4759 GGTGCC G^G[CT][GA]CC
BciVI 1278 GTATCC GTATCC(6/5)
BsaBI 3790 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2694 ACCGGA [AT]^CCGG[AT]
BspDI 1778 ATCGAT AT^CGAT
BspLU11I 393 ACATGT A^CATGT
BstXI 3813 CCAACCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1778 ATCGAT AT^CGAT
KspI 2484 CCGCGG CCGC^GG
MamI 3790 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1274 ACGCGT A^CGCGT
NcoI 498 CCATGG C^CATGG
PaeR7I 3074 CTCGAG C^TCGAG
SacII 2484 CCGCGG CCGC^GG
ScaI 279 AGTACT AGT^ACT
SexAI 4515 ACCTGGT A^CC[AT]GGT
SpeI 4043 ACTAGT A^CTAGT
XhoI 3074 CTCGAG C^TCGAG
XmnI 1036 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 2606 GACGTC G[GA]^CG[CT]C
3050 GACGCC
AflIII 393 ACATGT A^C[GA][CT]GT
1274 ACGCGT
AocI 1109 CCTGAGG CC^T[ACGT]AGG
3751 CCTGAGG
Aor51HI 1626 AGCGCT AGC^GCT
3216 AGCGCT
AviII 2049 TGCGCA TGC^GCA
2493 TGCGCA
BamHI 712 GGATCC G^GATCC
2024 GGATCC
BfaI 1505 CTAG C^TAG
4044 CTAG
BglII 315 AGATCT A^GATCT
3441 AGATCT
BsaHI 2606 GACGTC G[GA]^CG[CT]C
3050 GACGCC
BsmBI 2966 GAGACG CGTCTC(1/5)
3044 GAGACG
BsrDI 138 GCAATG GCAATG(2/0)
1002 GCAATG
Bsu36I 1109 CCTGAGG CC^T[ACGT]AGG
3751 CCTGAGG
DrdI 3890 GACGATGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4000 GACAGAGCGGTC
Eco47III 1626 AGCGCT AGC^GCT
3216 AGCGCT
Eco81I 1109 CCTGAGG CC^T[ACGT]AGG
3751 CCTGAGG
FspI 2049 TGCGCA TGC^GCA
2493 TGCGCA
Hin1I 2606 GACGTC G[GA]^CG[CT]C
3050 GACGCC
MaeI 1505 CTAG C^TAG
4044 CTAG
NaeI 665 GCCGGC GCC^GGC
2375 GCCGGC
NgoMIV 665 GCCGGC G^CCGGC
2375 GCCGGC
NspI 393 ACATGT [GA]CATG^[CT]
531 GCATGT
PflMI 2845 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3487 CCATGAGCTGG
SbfI 466 CCTGCAGG CCTGCA^GG
3430 CCTGCAGG
Sse8387I 466 CCTGCAGG CCTGCA^GG
3430 CCTGCAGG
TfiI 1654 GATTC G^A[AT]TC
3880 GATTC
Tsp509I 1394 AATT ^AATT
4823 AATT
Van91I 2845 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3487 CCATGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AspI AvaI AvaII
BalI BanII BbsI BbvCI BbvI
BcgI BclI BcnI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflFI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I TspRI
Tth111I TthHB8I XcmI XhoII