##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AccIII AclI AflII AgeI Alw44I
ApaI ApaLI AscI AseI AsnI
AspI AviII BbeI BbrPI BciVI
BfrI BglII BlpI Bpu1102I BsaBI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BsrFI
BsrGI BssHII BstAPI BstBI CelII
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco52I EcoRV EcoT22I FseI
FspI HpaI KasI KspI MamI
MfeI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI Tth111I
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 461 GACGTC GACGT^C
Acc65I 2495 GGTACC G^GTACC
AhdI 461 GACGTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1294 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1188 CCTTAGG CC^T[ACGT]AGG
Aor51HI 184 AGCGCT AGC^GCT
Asp700I 1179 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2495 GGTACC G^GTACC
AspEI 461 GACGTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 579 CCTAGG C^CTAGG
BcgI 324 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2336 TGATCA T^GATCA
BglI 1218 GCCTTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 579 CCTAGG C^CTAGG
BsaAI 1 TACGTG [CT]AC^GT[GA]
BsmI 1827 GAATGC GAATGC(1/-1)
BspLU11I 1843 ACATGT A^CATGT
BsrBI 412 CCGCTC CCGCTC(-3/-3)
BssSI 1495 CTCGTG CACGAG(-5/-1)
Bst1107I 3393 GTATAC GTA^TAC
BstEII 2949 GGTCACC G^GT[ACGT]ACC
BstPI 2949 GGTCACC G^GT[ACGT]ACC
BstZ17I 3393 GTATAC GTA^TAC
Bsu36I 1188 CCTTAGG CC^T[ACGT]AGG
DraIII 1075 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 461 GACGTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 184 AGCGCT AGC^GCT
Eco81I 1188 CCTTAGG CC^T[ACGT]AGG
EcoO65I 2949 GGTCACC G^GT[ACGT]ACC
EcoRI 3532 GAATTC G^AATTC
FbaI 2336 TGATCA T^GATCA
HindIII 762 AAGCTT A^AGCTT
KpnI 2495 GGTACC GGTAC^C
MluI 11 ACGCGT A^CGCGT
MspA1I 3221 CAGCTG C[AC]G^C[GT]G
PaeR7I 603 CTCGAG C^TCGAG
PstI 40 CTGCAG CTGCA^G
PvuII 3221 CAGCTG CAG^CTG
SacI 2269 GAGCTC GAGCT^C
SapI 270 GAAGAGC GCTCTTC(1/4)
SfcI 40 CTGCAG C^T[GA][CT]AG
SmaI 312 CCCGGG CCC^GGG
SspI 1312 AATATT AAT^ATT
SwaI 2206 ATTTAAAT ATTT^AAAT
XbaI 3473 TCTAGA T^CTAGA
XcmI 715 CCAACACAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 603 CTCGAG C^TCGAG
XmaCI 312 CCCGGG C^CCGGG
XmaI 312 CCCGGG C^CCGGG
XmnI 1179 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 700 GTCTAC GT^[AC][GT]AC
3393 GTATAC
AcyI 461 GACGTC G[GA]^CG[CT]C
777 GGCGTC
AflIII 11 ACGCGT A^C[GA][CT]GT
1843 ACATGT
BamHI 1696 GGATCC G^GATCC
1751 GGATCC
BsaHI 461 GACGTC G[GA]^CG[CT]C
777 GGCGTC
BsaI 2330 GGTCTC GGTCTC(1/5)
3510 GGTCTC
BspHI 441 TCATGA T^CATGA
936 TCATGA
BsrDI 1657 CATTGC GCAATG(2/0)
275 GCAATG
BstXI 358 CCAAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3606 CCATGGTGATGG
EarI 270 GAAGAG CTCTTC(1/4)
1353 GAAGAG
FauI 344 CCCGC CCCGC(4/6)
1050 CCCGC
HgaI 13 GCGTC GACGC(5/10)
778 GCGTC
Hin1I 461 GACGTC G[GA]^CG[CT]C
777 GGCGTC
HincII 100 GTCAAC GT[CT]^[GA]AC
2180 GTTGAC
HindII 100 GTCAAC GT[CT]^[GA]AC
2180 GTTGAC
Ksp632I 270 GAAGAG CTCTTC(1/4)
1353 GAAGAG
NcoI 2956 CCATGG C^CATGG
3606 CCATGG
RcaI 441 TCATGA T^CATGA
936 TCATGA
SphI 866 GCATGC GCATG^C
1370 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII