##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspHI AvrII BamHI
BcgI BciVI BclI BfrI BglI
BlnI BsiEI BsiHKAI BsiWI BspDI
BspHI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstEII BstPI BstXI
BstZ17I Cfr10I ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HpaI KpnI KspI MluI
NaeI NdeI NgoMIV NheI NotI
NsiI PaeR7I PinAI PmeI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SgrAI SnaBI SpeI Sse8387I
SwaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 377 GTAGAC GT^[AC][GT]AC
AcyI 1794 GGCGCC G[GA]^CG[CT]C
AflIII 2189 ACGTGT A^C[GA][CT]GT
AhdI 380 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1208 CCTTAGG CC^T[ACGT]AGG
AspEI 380 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1862 TGCGCA TGC^GCA
BbeI 1794 GGCGCC GGCGC^C
BbrPI 2188 CACGTG CAC^GTG
BglII 2070 AGATCT A^GATCT
BlpI 1977 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1977 GCTCAGC GC^T[ACGT]AGC
BsaHI 1794 GGCGCC G[GA]^CG[CT]C
BsmBI 428 GAGACG CGTCTC(1/5)
BspMI 1912 ACCTGC ACCTGC(4/8)
BsrDI 1348 CATTGC GCAATG(2/0)
BstBI 704 TTCGAA TT^CGAA
Bsu36I 1208 CCTTAGG CC^T[ACGT]AGG
CelII 1977 GCTCAGC GC^T[ACGT]AGC
Eam1105I 380 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1208 CCTTAGG CC^T[ACGT]AGG
EcoNI 2231 CCTCCGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1862 TGCGCA TGC^GCA
HaeII 1794 GGCGCC [GA]GCGC^[CT]
Hin1I 1794 GGCGCC G[GA]^CG[CT]C
HincII 1956 GTCAAC GT[CT]^[GA]AC
HindII 1956 GTCAAC GT[CT]^[GA]AC
HindIII 1 AAGCTT A^AGCTT
KasI 1794 GGCGCC G^GCGCC
MfeI 1950 CAATTG C^AATTG
MunI 1950 CAATTG C^AATTG
NarI 1794 GGCGCC GG^CGCC
NspI 1289 GCATGC [GA]CATG^[CT]
NspV 704 TTCGAA TT^CGAA
PacI 866 TTAATTAA TTAAT^TAA
PflMI 1961 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2188 CACGTG CAC^GTG
PmlI 2188 CACGTG CAC^GTG
ScaI 1671 AGTACT AGT^ACT
SfoI 1794 GGCGCC GGC^GCC
SfuI 704 TTCGAA TT^CGAA
SmaI 922 CCCGGG CCC^GGG
SphI 1289 GCATGC GCATG^C
StuI 2840 AGGCCT AGG^CCT
Van91I 1961 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 922 CCCGGG C^CCGGG
XmaI 922 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 1894 TCCGGA T^CCGGA
3713 TCCGGA
AseI 2487 ATTAAT AT^TAAT
3372 ATTAAT
AsnI 2487 ATTAAT AT^TAAT
3372 ATTAAT
AspI 460 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3131 GACTCTGTC
BanI 1794 GGCGCC G^G[CT][GA]CC
2924 GGCACC
BbvCI 2682 CCTCAGC CCTCAGC(-5/-2)
2877 CCTCAGC
BsaAI 1437 TACGTG [CT]AC^GT[GA]
2188 CACGTG
BsaBI 1482 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1602 GATATGAATC
BseAI 1894 TCCGGA T^CCGGA
3713 TCCGGA
BsmFI 56 GGGAC GGGAC(10/14)
197 GTCCC
BsmI 1577 GAATGC GAATGC(1/-1)
2363 GAATGC
Bsp1407I 16 TGTACA T^GTACA
535 TGTACA
BspEI 1894 TCCGGA T^CCGGA
3713 TCCGGA
BsrGI 16 TGTACA T^GTACA
535 TGTACA
BstYI 2070 AGATCT [GA]^GATC[CT]
2615 AGATCC
DraI 723 TTTAAA TTT^AAA
1175 TTTAAA
MamI 1482 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1602 GATATGAATC
MflI 2070 AGATCT [GA]^GATC[CT]
2615 AGATCC
MroI 1894 TCCGGA T^CCGGA
3713 TCCGGA
NruI 169 TCGCGA TCG^CGA
266 TCGCGA
PflFI 460 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3131 GACTCTGTC
PshBI 2487 ATTAAT AT^TAAT
3372 ATTAAT
SapI 3342 GAAGAGC GCTCTTC(1/4)
31 GCTCTTC
SfcI 1459 CTACAG C^T[GA][CT]AG
3775 CTGTAG
SmlI 2542 CTCAAG C^T[CT][GA]AG
3201 CTCAAG
SspBI 16 TGTACA T^GTACA
535 TGTACA
SspI 258 AATATT AAT^ATT
354 AATATT
Tth111I 460 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3131 GACTCTGTC
XbaI 750 TCTAGA T^CTAGA
2820 TCTAGA
XhoII 2070 AGATCT [GA]^GATC[CT]
2615 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaI AvaII
BalI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseRI BsgI BsiYI
BslI BsmAI BsoBI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I