##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AatII Acc65I AcyI AgeI ApaI
AscI Asp718I AviII BbeI BbrPI
BglI BsaHI BsaI BsiWI BsrFI
BssHII BstBI Cfr10I CpoI FseI
FspI Hin1I KasI KpnI MluI
NaeI NarI NgoMIV NotI NruI
NspV PinAI PmaCI PmeI PmlI
PpuMI PshAI RsrII SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AclI 11330 AACGTT AA^CGTT
AhdI 7894 GACAATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 7894 GACAATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1274 CTCGAG C^[CT]CG[GA]G
AvrII 3856 CCTAGG C^CTAGG
BbvCI 5770 CCTCAGC CCTCAGC(-5/-2)
BlnI 3856 CCTAGG C^CTAGG
BsoBI 1274 CTCGAG C^[CT]CG[GA]G
BspDI 5353 ATCGAT AT^CGAT
BsrBI 8216 GAGCGG CCGCTC(-3/-3)
BstEII 13038 GGTTACC G^GT[ACGT]ACC
BstPI 13038 GGTTACC G^GT[ACGT]ACC
ClaI 5353 ATCGAT AT^CGAT
DraIII 3959 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4351 GACAATAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4266 CGGCCG C^GGCCG
Eam1105I 7894 GACAATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4266 CGGCCG C^GGCCG
Eco52I 4266 CGGCCG C^GGCCG
EcoO65I 13038 GGTTACC G^GT[ACGT]ACC
KspI 88 CCGCGG CCGC^GG
NheI 10494 GCTAGC G^CTAGC
PacI 9721 TTAATTAA TTAAT^TAA
PaeR7I 1274 CTCGAG C^TCGAG
Psp1406I 11330 AACGTT AA^CGTT
PvuI 12393 CGATCG CGAT^CG
SacII 88 CCGCGG CCGC^GG
SalI 12815 GTCGAC G^TCGAC
SexAI 11450 ACCAGGT A^CC[AT]GGT
SpeI 3865 ACTAGT A^CTAGT
StuI 9966 AGGCCT AGG^CCT
XhoI 1274 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccII 71 CGCG CG^CG
89 CGCG
AccIII 140 TCCGGA T^CCGGA
12984 TCCGGA
AflII 6925 CTTAAG C^TTAAG
8449 CTTAAG
Alw44I 7154 GTGCAC G^TGCAC
10601 GTGCAC
AocI 348 CCTCAGG CC^T[ACGT]AGG
11842 CCTCAGG
Aor51HI 1118 AGCGCT AGC^GCT
2345 AGCGCT
ApaLI 7154 GTGCAC G^TGCAC
10601 GTGCAC
AspI 8876 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
9885 GACCAAGTC
BalI 3408 TGGCCA TGG^CCA
3629 TGGCCA
BamHI 4944 GGATCC G^GATCC
7738 GGATCC
BanI 1583 GGTGCC G^G[CT][GA]CC
11923 GGTGCC
BcgI 3570 CGAGAGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
10917 GCATTTTGTTCG
BfrI 6925 CTTAAG C^TTAAG
8449 CTTAAG
BsaAI 3989 TACGTG [CT]AC^GT[GA]
11027 TACGTG
BseAI 140 TCCGGA T^CCGGA
12984 TCCGGA
BsmBI 3841 CGTCTC CGTCTC(1/5)
10218 GAGACG
BspEI 140 TCCGGA T^CCGGA
12984 TCCGGA
BssSI 695 CACGAG CACGAG(-5/-1)
6845 CACGAG
Bst1107I 5148 GTATAC GTA^TAC
7231 GTATAC
BstUI 71 CGCG CG^CG
89 CGCG
BstZ17I 5148 GTATAC GTA^TAC
7231 GTATAC
Bsu36I 348 CCTCAGG CC^T[ACGT]AGG
11842 CCTCAGG
DraII 2563 AGGCCCC [GA]G^G[ACGT]CC[CT]
5401 GGGGCCT
Eco47III 1118 AGCGCT AGC^GCT
2345 AGCGCT
Eco81I 348 CCTCAGG CC^T[ACGT]AGG
11842 CCTCAGG
EcoO109I 2563 AGGCCCC [GA]G^G[ACGT]CC[CT]
5401 GGGGCCT
FauI 87 CCCGC CCCGC(4/6)
11510 CCCGC
HpaI 6466 GTTAAC GTT^AAC
10366 GTTAAC
MfeI 809 CAATTG C^AATTG
3914 CAATTG
MluNI 3408 TGGCCA TGG^CCA
3629 TGGCCA
MroI 140 TCCGGA T^CCGGA
12984 TCCGGA
MscI 3408 TGGCCA TGG^CCA
3629 TGGCCA
MunI 809 CAATTG C^AATTG
3914 CAATTG
MvnI 71 CGCG CG^CG
89 CGCG
PflFI 8876 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
9885 GACCAAGTC
SwaI 3944 ATTTAAAT ATTT^AAAT
4220 ATTTAAAT
Tth111I 8876 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
9885 GACCAAGTC
XcmI 3375 CCAACTAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
7698 CCAATGGGTTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaII BanII
BbsI BbvI BciVI BclI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaBI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspHI BspLU11I BspMI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DsaI EaeI EarI EcoNI EcoRI
EcoRII EcoRV EcoT14I EcoT22I FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PshBI PstI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SphI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XhoII XmnI