##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AclI AcyI AhdI
AscI AspEI AviII AvrII BbeI
BbrPI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BseAI BsiWI BsmBI
BspEI BspHI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I CelII CpoI
Eam1105I FseI FspI HgaI Hin1I
KasI MluI MroI NarI NheI
NotI NruI NspV PmaCI PmeI
PmlI Psp1406I RcaI RsrII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 2869 GGTACC G^GTACC
AccI 943 GTAGAC GT^[AC][GT]AC
AflII 1051 CTTAAG C^TTAAG
AgeI 2376 ACCGGT A^CCGGT
Alw44I 3709 GTGCAC G^TGCAC
Aor51HI 2960 AGCGCT AGC^GCT
ApaLI 3709 GTGCAC G^TGCAC
AseI 438 ATTAAT AT^TAAT
AsnI 438 ATTAAT AT^TAAT
Asp718I 2869 GGTACC G^GTACC
AspI 1632 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 2771 CGATCGGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1862 GTATCC GTATCC(6/5)
BfrI 1051 CTTAAG C^TTAAG
BsmI 3901 GCATTC GAATGC(1/-1)
Bsp1407I 854 TGTACA T^GTACA
BspDI 2327 ATCGAT AT^CGAT
BsrBI 4407 GAGCGG CCGCTC(-3/-3)
BsrDI 2451 CATTGC GCAATG(2/0)
BsrGI 854 TGTACA T^GTACA
ClaI 2327 ATCGAT AT^CGAT
DraIII 252 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3544 GACCTCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2960 AGCGCT AGC^GCT
HincII 3138 GTTAAC GT[CT]^[GA]AC
HindII 3138 GTTAAC GT[CT]^[GA]AC
HindIII 4450 AAGCTT A^AGCTT
HpaI 3138 GTTAAC GTT^AAC
KpnI 2869 GGTACC GGTAC^C
MfeI 3359 CAATTG C^AATTG
MunI 3359 CAATTG C^AATTG
NaeI 3578 GCCGGC GCC^GGC
NdeI 3227 CATATG CA^TATG
NgoMIV 3578 GCCGGC G^CCGGC
PacI 439 TTAATTAA TTAAT^TAA
PaeR7I 4295 CTCGAG C^TCGAG
PflFI 1632 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2376 ACCGGT A^CCGGT
PshAI 3473 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 438 ATTAAT AT^TAAT
PvuI 2771 CGATCG CGAT^CG
SacI 4290 GAGCTC GAGCT^C
SapI 2238 GCTCTTC GCTCTTC(1/4)
SexAI 1767 ACCTGGT A^CC[AT]GGT
SspBI 854 TGTACA T^GTACA
StuI 2804 AGGCCT AGG^CCT
Tth111I 1632 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4295 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AocI 119 CCTGAGG CC^T[ACGT]AGG
1899 CCTCAGG
ApaI 1227 GGGCCC GGGCC^C
3342 GGGCCC
AvaI 1866 CCCGAG C^[CT]CG[GA]G
4295 CTCGAG
BamHI 1353 GGATCC G^GATCC
2948 GGATCC
BbvCI 40 CCTCAGC CCTCAGC(-5/-2)
4168 CCTCAGC
BglI 58 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
117 GCCCTGAGGGC
BsaBI 3648 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4151 GATTGATATC
BsaI 3542 GAGACC GGTCTC(1/5)
1672 GGTCTC
BsaWI 2213 ACCGGA [AT]^CCGG[AT]
2376 ACCGGT
BsoBI 1866 CCCGAG C^[CT]CG[GA]G
4295 CTCGAG
BspLU11I 294 ACATGT A^CATGT
857 ACATGT
Bsu36I 119 CCTGAGG CC^T[ACGT]AGG
1899 CCTCAGG
EagI 2 CGGCCG C^GGCCG
2103 CGGCCG
EclXI 2 CGGCCG C^GGCCG
2103 CGGCCG
Eco52I 2 CGGCCG C^GGCCG
2103 CGGCCG
Eco81I 119 CCTGAGG CC^T[ACGT]AGG
1899 CCTCAGG
EcoRV 4155 GATATC GAT^ATC
4382 GATATC
EcoT22I 2905 ATGCAT ATGCA^T
3899 ATGCAT
MamI 3648 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4151 GATTGATATC
NcoI 59 CCATGG C^CATGG
3603 CCATGG
NsiI 2905 ATGCAT ATGCA^T
3899 ATGCAT
PvuII 1736 CAGCTG CAG^CTG
2739 CAGCTG
ScaI 2576 AGTACT AGT^ACT
3455 AGTACT
SphI 1888 GCATGC GCATG^C
2777 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BalI BanI BanII
BbsI BbvI BclI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoII XmnI