################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AccIII AclI AgeI AhdI AocI Aor51HI AspEI AviII AvrII BbrPI BciVI BclI BglII BlnI BsaBI BsaWI BseAI BsiWI BsmBI Bsp1407I BspEI BsrBI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI FbaI FseI FspI HpaI MamI MfeI MluI MroI MunI NdeI NheI NotI NspV PacI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I RsrII SalI SbfI SexAI SfuI SgrAI SpeI SphI Sse8387I SspBI XcmI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 760 GTCTAC GT^[AC][GT]AC AflII 1967 CTTAAG C^TTAAG Alw44I 405 GTGCAC G^TGCAC ApaI 2163 GGGCCC GGGCC^C ApaLI 405 GTGCAC G^TGCAC AscI 840 GGCGCGCC GG^CGCGCC AseI 2532 ATTAAT AT^TAAT AsnI 2532 ATTAAT AT^TAAT Asp700I 2297 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1605 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2598 TGGCCA TGG^CCA BamHI 2779 GGATCC G^GATCC BbvCI 31 GCTGAGG CCTCAGC(-5/-2) BcgI 929 CGAGGCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1967 CTTAAG C^TTAAG BsaAI 1723 TACGTA [CT]AC^GT[GA] BspDI 1056 ATCGAT AT^CGAT BspHI 3391 TCATGA T^CATGA BstXI 1677 CCAAAGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 1056 ATCGAT AT^CGAT DraI 2321 TTTAAA TTT^AAA EarI 2617 GAAGAG CTCTTC(1/4) EcoRV 1331 GATATC GAT^ATC EcoT22I 2316 ATGCAT ATGCA^T HgaI 2422 GACGC GACGC(5/10) HincII 3452 GTCAAC GT[CT]^[GA]AC HindII 3452 GTCAAC GT[CT]^[GA]AC HindIII 3330 AAGCTT A^AGCTT Ksp632I 2617 GAAGAG CTCTTC(1/4) KspI 38 CCGCGG CCGC^GG MluNI 2598 TGGCCA TGG^CCA MscI 2598 TGGCCA TGG^CCA NaeI 373 GCCGGC GCC^GGC NgoMIV 373 GCCGGC G^CCGGC NruI 1008 TCGCGA TCG^CGA NsiI 2316 ATGCAT ATGCA^T PaeR7I 927 CTCGAG C^TCGAG PflFI 1605 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 2532 ATTAAT AT^TAAT PvuI 1058 CGATCG CGAT^CG RcaI 3391 TCATGA T^CATGA SacI 3376 GAGCTC GAGCT^C SacII 38 CCGCGG CCGC^GG SapI 2617 GAAGAGC GCTCTTC(1/4) ScaI 1681 AGTACT AGT^ACT SfiI 354 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 2166 CCCGGG CCC^GGG SnaBI 1723 TACGTA TAC^GTA SspI 3143 AATATT AAT^ATT SwaI 2320 ATTTAAAT ATTT^AAAT TfiI 1942 GAATC G^A[AT]TC Tth111I 1605 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 927 CTCGAG C^TCGAG XmaCI 2166 CCCGGG C^CCGGG XmaI 2166 CCCGGG C^CCGGG XmnI 2297 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Acc65I 416 GGTACC G^GTACC 3572 GGTACC AlwNI 573 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG 1025 CAGCCTCTG Asp718I 416 GGTACC G^GTACC 3572 GGTACC BbsI 1075 GAAGAC GAAGAC(2/6) 234 GTCTTC BlpI 1168 GCTCAGC GC^T[ACGT]AGC 2918 GCTGAGC Bpu1102I 1168 GCTCAGC GC^T[ACGT]AGC 2918 GCTGAGC BpuAI 1075 GAAGAC GAAGAC(2/6) 234 GTCTTC BsiEI 1058 CGATCG CG[GA][CT]^CG 2676 CGGTCG BspMI 138 GCAGGT ACCTGC(4/8) 726 GCAGGT BsrFI 373 GCCGGC [GA]^CCGG[CT] 933 GCCGGT CelII 1168 GCTCAGC GC^T[ACGT]AGC 2918 GCTGAGC Cfr10I 373 GCCGGC [GA]^CCGG[CT] 933 GCCGGT DraII 353 AGGCCCT [GA]G^G[ACGT]CC[CT] 2162 AGGGCCC DraIII 456 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG 510 CACAGTGTG EcoO109I 353 AGGCCCT [GA]G^G[ACGT]CC[CT] 2162 AGGGCCC KpnI 416 GGTACC GGTAC^C 3572 GGTACC PflMI 673 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1110 CCAGGAAATGG PvuII 1558 CAGCTG CAG^CTG 2612 CAGCTG StuI 923 AGGCCT AGG^CCT 1821 AGGCCT Van91I 673 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1110 CCAGGAAATGG XbaI 2101 TCTAGA T^CTAGA 2219 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI ApoI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII