##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
AocI Aor51HI AspEI AviII AvrII
BbrPI BciVI BclI BglII BlnI
BsaBI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspEI BsrBI BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI FbaI FseI
FspI HpaI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NspV PacI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I RsrII
SalI SbfI SexAI SfuI SgrAI
SpeI SphI Sse8387I SspBI XcmI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 760 GTCTAC GT^[AC][GT]AC
AflII 1967 CTTAAG C^TTAAG
Alw44I 405 GTGCAC G^TGCAC
ApaI 2163 GGGCCC GGGCC^C
ApaLI 405 GTGCAC G^TGCAC
AscI 840 GGCGCGCC GG^CGCGCC
AseI 2532 ATTAAT AT^TAAT
AsnI 2532 ATTAAT AT^TAAT
Asp700I 2297 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1605 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2598 TGGCCA TGG^CCA
BamHI 2779 GGATCC G^GATCC
BbvCI 31 GCTGAGG CCTCAGC(-5/-2)
BcgI 929 CGAGGCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1967 CTTAAG C^TTAAG
BsaAI 1723 TACGTA [CT]AC^GT[GA]
BspDI 1056 ATCGAT AT^CGAT
BspHI 3391 TCATGA T^CATGA
BstXI 1677 CCAAAGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1056 ATCGAT AT^CGAT
DraI 2321 TTTAAA TTT^AAA
EarI 2617 GAAGAG CTCTTC(1/4)
EcoRV 1331 GATATC GAT^ATC
EcoT22I 2316 ATGCAT ATGCA^T
HgaI 2422 GACGC GACGC(5/10)
HincII 3452 GTCAAC GT[CT]^[GA]AC
HindII 3452 GTCAAC GT[CT]^[GA]AC
HindIII 3330 AAGCTT A^AGCTT
Ksp632I 2617 GAAGAG CTCTTC(1/4)
KspI 38 CCGCGG CCGC^GG
MluNI 2598 TGGCCA TGG^CCA
MscI 2598 TGGCCA TGG^CCA
NaeI 373 GCCGGC GCC^GGC
NgoMIV 373 GCCGGC G^CCGGC
NruI 1008 TCGCGA TCG^CGA
NsiI 2316 ATGCAT ATGCA^T
PaeR7I 927 CTCGAG C^TCGAG
PflFI 1605 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2532 ATTAAT AT^TAAT
PvuI 1058 CGATCG CGAT^CG
RcaI 3391 TCATGA T^CATGA
SacI 3376 GAGCTC GAGCT^C
SacII 38 CCGCGG CCGC^GG
SapI 2617 GAAGAGC GCTCTTC(1/4)
ScaI 1681 AGTACT AGT^ACT
SfiI 354 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2166 CCCGGG CCC^GGG
SnaBI 1723 TACGTA TAC^GTA
SspI 3143 AATATT AAT^ATT
SwaI 2320 ATTTAAAT ATTT^AAAT
TfiI 1942 GAATC G^A[AT]TC
Tth111I 1605 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 927 CTCGAG C^TCGAG
XmaCI 2166 CCCGGG C^CCGGG
XmaI 2166 CCCGGG C^CCGGG
XmnI 2297 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 416 GGTACC G^GTACC
3572 GGTACC
AlwNI 573 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
1025 CAGCCTCTG
Asp718I 416 GGTACC G^GTACC
3572 GGTACC
BbsI 1075 GAAGAC GAAGAC(2/6)
234 GTCTTC
BlpI 1168 GCTCAGC GC^T[ACGT]AGC
2918 GCTGAGC
Bpu1102I 1168 GCTCAGC GC^T[ACGT]AGC
2918 GCTGAGC
BpuAI 1075 GAAGAC GAAGAC(2/6)
234 GTCTTC
BsiEI 1058 CGATCG CG[GA][CT]^CG
2676 CGGTCG
BspMI 138 GCAGGT ACCTGC(4/8)
726 GCAGGT
BsrFI 373 GCCGGC [GA]^CCGG[CT]
933 GCCGGT
CelII 1168 GCTCAGC GC^T[ACGT]AGC
2918 GCTGAGC
Cfr10I 373 GCCGGC [GA]^CCGG[CT]
933 GCCGGT
DraII 353 AGGCCCT [GA]G^G[ACGT]CC[CT]
2162 AGGGCCC
DraIII 456 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
510 CACAGTGTG
EcoO109I 353 AGGCCCT [GA]G^G[ACGT]CC[CT]
2162 AGGGCCC
KpnI 416 GGTACC GGTAC^C
3572 GGTACC
PflMI 673 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1110 CCAGGAAATGG
PvuII 1558 CAGCTG CAG^CTG
2612 CAGCTG
StuI 923 AGGCCT AGG^CCT
1821 AGGCCT
Van91I 673 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1110 CCAGGAAATGG
XbaI 2101 TCTAGA T^CTAGA
2219 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AspHI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspLU11I
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII