##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccIII AcyI AgeI AlwNI
Aor51HI AscI AspI AviII BamHI
BbeI BbrPI BcgI BciVI BcnI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspMI
BsrFI Bst1107I BstBI BstEII BstPI
BstZ17I Cfr10I ClaI CpoI DrdI
DsaI EagI EclXI Eco47III Eco52I
EcoO65I EcoT22I FseI FspI HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII KasI KspI MamI
MfeI MluI MroI MspI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PvuI RsrII SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1127 GGTACC G^GTACC
AccI 3408 GTAGAC GT^[AC][GT]AC
AccII 2813 CGCG CG^CG
AclI 1504 AACGTT AA^CGTT
AflII 2687 CTTAAG C^TTAAG
AhdI 2273 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 203 CCTCAGG CC^T[ACGT]AGG
Asp718I 1127 GGTACC G^GTACC
AspEI 2273 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1691 CCTAGG C^CTAGG
BbvCI 1325 GCTGAGG CCTCAGC(-5/-2)
BclI 3376 TGATCA T^GATCA
BfrI 2687 CTTAAG C^TTAAG
BlnI 1691 CCTAGG C^CTAGG
BlpI 1865 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1865 GCTGAGC GC^T[ACGT]AGC
BseRI 1349 GAGGAG GAGGAG(10/8)
BsmBI 485 GAGACG CGTCTC(1/5)
Bsp1407I 356 TGTACA T^GTACA
BsrBI 296 GAGCGG CCGCTC(-3/-3)
BsrDI 1816 CATTGC GCAATG(2/0)
BsrGI 356 TGTACA T^GTACA
BssHII 2812 GCGCGC G^CGCGC
BssSI 3269 CTCGTG CACGAG(-5/-1)
BstAPI 2156 GCACTCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2813 CGCG CG^CG
Bsu36I 203 CCTCAGG CC^T[ACGT]AGG
CelII 1865 GCTGAGC GC^T[ACGT]AGC
Eam1105I 2273 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2892 GAAGAG CTCTTC(1/4)
Eco81I 203 CCTCAGG CC^T[ACGT]AGG
EcoRV 3688 GATATC GAT^ATC
FauI 559 CCCGC CCCGC(4/6)
FbaI 3376 TGATCA T^GATCA
HaeII 2749 GGCGCT [GA]GCGC^[CT]
KpnI 1127 GGTACC GGTAC^C
Ksp632I 2892 GAAGAG CTCTTC(1/4)
MspA1I 3301 CAGCTG C[AC]G^C[GT]G
MvnI 2813 CGCG CG^CG
PflMI 1839 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1504 AACGTT AA^CGTT
PvuII 3301 CAGCTG CAG^CTG
SexAI 819 ACCAGGT A^CC[AT]GGT
SpeI 375 ACTAGT A^CTAGT
SphI 2765 GCATGC GCATG^C
SspBI 356 TGTACA T^GTACA
Van91I 1839 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2095 TCTAGA T^CTAGA
XcmI 2130 CCACCGTGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 2794 ACATGT A^C[GA][CT]GT
3857 ACATGT
Alw44I 1879 GTGCAC G^TGCAC
2135 GTGCAC
ApaI 1720 GGGCCC GGGCC^C
1727 GGGCCC
ApaLI 1879 GTGCAC G^TGCAC
2135 GTGCAC
AseI 2498 ATTAAT AT^TAAT
3761 ATTAAT
AsnI 2498 ATTAAT AT^TAAT
3761 ATTAAT
Asp700I 3050 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3109 GAAGACATTC
BalI 505 TGGCCA TGG^CCA
2362 TGGCCA
BanI 1127 GGTACC G^G[CT][GA]CC
1765 GGTGCC
BglI 1843 GCCTTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2206 GCCACAGAGGC
BsgI 2005 GTGCAG GTGCAG(16/14)
3660 GTGCAG
BsmI 3368 GAATGC GAATGC(1/-1)
188 GCATTC
BspHI 118 TCATGA T^CATGA
868 TCATGA
BspLU11I 2794 ACATGT A^CATGT
3857 ACATGT
EcoNI 31 CCTTAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1991 CCTCTGTTAGG
EcoRI 105 GAATTC G^AATTC
1559 GAATTC
MluNI 505 TGGCCA TGG^CCA
2362 TGGCCA
MscI 505 TGGCCA TGG^CCA
2362 TGGCCA
PshBI 2498 ATTAAT AT^TAAT
3761 ATTAAT
RcaI 118 TCATGA T^CATGA
868 TCATGA
SacI 333 GAGCTC GAGCT^C
3415 GAGCTC
ScaI 2703 AGTACT AGT^ACT
3475 AGTACT
SspI 391 AATATT AAT^ATT
2771 AATATT
Tsp45I 1376 GTCAC ^GT[GC]AC
2258 GTGAC
XmnI 3050 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3109 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BanII
BbsI BbvI BfaI BglII BmyI
BpmI BpuAI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII