##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccIII AfaI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AviII BbrPI BbsI BcgI
BciVI BfrI BglII BlpI Bpu1102I
BpuAI BsaAI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoRI EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfaNI SfiI SfuI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
SspBI SspI SwaI XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AclI 227 AACGTT AA^CGTT
AcsI 1735 AAATTT [GA]^AATT[CT]
AhdI 1718 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 944 GGGCCC GGGCC^C
ApoI 1735 AAATTT [GA]^AATT[CT]
AspEI 1718 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 247 GAGCAC G[AT]GC[AT]^C
AspI 1872 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 107 CCCGAG C^[CT]CG[GA]G
AvrII 1409 CCTAGG C^CTAGG
BalI 338 TGGCCA TGG^CCA
BamHI 1273 GGATCC G^GATCC
BbvCI 1798 GCTGAGG CCTCAGC(-5/-2)
BglI 38 GCCTTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1409 CCTAGG C^CTAGG
BpmI 1792 CTGGAG CTGGAG(16/14)
BsaBI 1309 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 247 GAGCAC G[AT]GC[AT]^C
BsoBI 107 CCCGAG C^[CT]CG[GA]G
BsrDI 342 CATTGC GCAATG(2/0)
BsrFI 1646 GCCGGC [GA]^CCGG[CT]
BstEII 1213 GGTTACC G^GT[ACGT]ACC
BstPI 1213 GGTTACC G^GT[ACGT]ACC
BstYI 1273 GGATCC [GA]^GATC[CT]
Cfr10I 1646 GCCGGC [GA]^CCGG[CT]
DsaI 7 CCGTGG C^C[GA][CT]GG
Eam1105I 1718 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 971 CCTGTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1213 GGTTACC G^GT[ACGT]ACC
EcoRV 1414 GATATC GAT^ATC
MamI 1309 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1273 GGATCC [GA]^GATC[CT]
MluNI 338 TGGCCA TGG^CCA
MscI 338 TGGCCA TGG^CCA
MslI 1104 CACACCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 216 CAGCGG C[AC]G^C[GT]G
NaeI 1646 GCCGGC GCC^GGC
NgoMIV 1646 GCCGGC G^CCGGC
NspI 500 ACATGC [GA]CATG^[CT]
PflFI 1872 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1716 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1321 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 227 AACGTT AA^CGTT
SexAI 1363 ACCAGGT A^CC[AT]GGT
SpeI 1384 ACTAGT A^CTAGT
StuI 992 AGGCCT AGG^CCT
Tth111I 1872 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 326 CCAAGAGGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1273 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 663 GTAGAC GT^[AC][GT]AC
984 GTAGAC
AccII 1607 CGCG CG^CG
1609 CGCG
AcyI 382 GGCGCC G[GA]^CG[CT]C
547 GGCGCC
BbeI 382 GGCGCC GGCGC^C
547 GGCGCC
BclI 1112 TGATCA T^GATCA
1833 TGATCA
BfaI 1385 CTAG C^TAG
1410 CTAG
BsaHI 382 GGCGCC G[GA]^CG[CT]C
547 GGCGCC
BseRI 1029 GAGGAG GAGGAG(10/8)
1041 GAGGAG
BsgI 1844 CTGCAC GTGCAG(16/14)
933 GTGCAG
BstUI 1607 CGCG CG^CG
1609 CGCG
BstXI 616 CCAGAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1631 CCAACACTTTGG
DraI 1476 TTTAAA TTT^AAA
1744 TTTAAA
EaeI 338 TGGCCA [CT]^GGCC[GA]
1601 CGGCCA
EarI 1728 CTCTTC CTCTTC(1/4)
1084 GAAGAG
FbaI 1112 TGATCA T^GATCA
1833 TGATCA
HaeII 382 GGCGCC [GA]GCGC^[CT]
547 GGCGCC
HgaI 463 GACGC GACGC(5/10)
437 GCGTC
Hin1I 382 GGCGCC G[GA]^CG[CT]C
547 GGCGCC
HphI 87 GGTGA GGTGA(8/7)
428 TCACC
KasI 382 GGCGCC G^GCGCC
547 GGCGCC
Ksp632I 1728 CTCTTC CTCTTC(1/4)
1084 GAAGAG
MaeI 1385 CTAG C^TAG
1410 CTAG
MvnI 1607 CGCG CG^CG
1609 CGCG
NarI 382 GGCGCC GG^CGCC
547 GGCGCC
NlaIII 501 CATG CATG^
1831 CATG
PstI 1227 CTGCAG CTGCA^G
1820 CTGCAG
SfcI 1227 CTGCAG C^T[GA][CT]AG
1820 CTGCAG
SfoI 382 GGCGCC GGC^GCC
547 GGCGCC
TaqI 245 TCGA T^CGA
749 TCGA
Tsp45I 523 GTGAC ^GT[GC]AC
1335 GTGAC
TthHB8I 245 TCGA T^CGA
749 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanI BanII BbvI BcnI BmyI
BsaI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIV PflMI PleI
Sau3AI Sau96I ScrFI StyI TfiI
Tru9I TseI Tsp509I TspRI Van91I