################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccIII AfaI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AviII BbrPI BbsI BcgI BciVI BfrI BglII BlpI Bpu1102I BpuAI BsaAI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoRI EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI KspI MfeI MluI MroI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfiI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI SspI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AclI 227 AACGTT AA^CGTT AcsI 1735 AAATTT [GA]^AATT[CT] AhdI 1718 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 944 GGGCCC GGGCC^C ApoI 1735 AAATTT [GA]^AATT[CT] AspEI 1718 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 247 GAGCAC G[AT]GC[AT]^C AspI 1872 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 107 CCCGAG C^[CT]CG[GA]G AvrII 1409 CCTAGG C^CTAGG BalI 338 TGGCCA TGG^CCA BamHI 1273 GGATCC G^GATCC BbvCI 1798 GCTGAGG CCTCAGC(-5/-2) BglI 38 GCCTTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1409 CCTAGG C^CTAGG BpmI 1792 CTGGAG CTGGAG(16/14) BsaBI 1309 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 247 GAGCAC G[AT]GC[AT]^C BsoBI 107 CCCGAG C^[CT]CG[GA]G BsrDI 342 CATTGC GCAATG(2/0) BsrFI 1646 GCCGGC [GA]^CCGG[CT] BstEII 1213 GGTTACC G^GT[ACGT]ACC BstPI 1213 GGTTACC G^GT[ACGT]ACC BstYI 1273 GGATCC [GA]^GATC[CT] Cfr10I 1646 GCCGGC [GA]^CCGG[CT] DsaI 7 CCGTGG C^C[GA][CT]GG Eam1105I 1718 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 971 CCTGTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1213 GGTTACC G^GT[ACGT]ACC EcoRV 1414 GATATC GAT^ATC MamI 1309 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1273 GGATCC [GA]^GATC[CT] MluNI 338 TGGCCA TGG^CCA MscI 338 TGGCCA TGG^CCA MslI 1104 CACACCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 216 CAGCGG C[AC]G^C[GT]G NaeI 1646 GCCGGC GCC^GGC NgoMIV 1646 GCCGGC G^CCGGC NspI 500 ACATGC [GA]CATG^[CT] PflFI 1872 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1716 GGGACCC [GA]G^G[AT]CC[CT] PshAI 1321 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 227 AACGTT AA^CGTT SexAI 1363 ACCAGGT A^CC[AT]GGT SpeI 1384 ACTAGT A^CTAGT StuI 992 AGGCCT AGG^CCT Tth111I 1872 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 326 CCAAGAGGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1273 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 663 GTAGAC GT^[AC][GT]AC 984 GTAGAC AccII 1607 CGCG CG^CG 1609 CGCG AcyI 382 GGCGCC G[GA]^CG[CT]C 547 GGCGCC BbeI 382 GGCGCC GGCGC^C 547 GGCGCC BclI 1112 TGATCA T^GATCA 1833 TGATCA BfaI 1385 CTAG C^TAG 1410 CTAG BsaHI 382 GGCGCC G[GA]^CG[CT]C 547 GGCGCC BseRI 1029 GAGGAG GAGGAG(10/8) 1041 GAGGAG BsgI 1844 CTGCAC GTGCAG(16/14) 933 GTGCAG BstUI 1607 CGCG CG^CG 1609 CGCG BstXI 616 CCAGAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1631 CCAACACTTTGG DraI 1476 TTTAAA TTT^AAA 1744 TTTAAA EaeI 338 TGGCCA [CT]^GGCC[GA] 1601 CGGCCA EarI 1728 CTCTTC CTCTTC(1/4) 1084 GAAGAG FbaI 1112 TGATCA T^GATCA 1833 TGATCA HaeII 382 GGCGCC [GA]GCGC^[CT] 547 GGCGCC HgaI 463 GACGC GACGC(5/10) 437 GCGTC Hin1I 382 GGCGCC G[GA]^CG[CT]C 547 GGCGCC HphI 87 GGTGA GGTGA(8/7) 428 TCACC KasI 382 GGCGCC G^GCGCC 547 GGCGCC Ksp632I 1728 CTCTTC CTCTTC(1/4) 1084 GAAGAG MaeI 1385 CTAG C^TAG 1410 CTAG MvnI 1607 CGCG CG^CG 1609 CGCG NarI 382 GGCGCC GG^CGCC 547 GGCGCC NlaIII 501 CATG CATG^ 1831 CATG PstI 1227 CTGCAG CTGCA^G 1820 CTGCAG SfcI 1227 CTGCAG C^T[GA][CT]AG 1820 CTGCAG SfoI 382 GGCGCC GGC^GCC 547 GGCGCC TaqI 245 TCGA T^CGA 749 TCGA Tsp45I 523 GTGAC ^GT[GC]AC 1335 GTGAC TthHB8I 245 TCGA T^CGA 749 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AluI AlwI AlwNI AvaII BanI BanII BbvI BcnI BmyI BsaI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIV PflMI PleI Sau3AI Sau96I ScrFI StyI TfiI Tru9I TseI Tsp509I TspRI Van91I