##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp700I AspEI
AviII AvrII BbeI BbrPI BcgI
BclI BfrI BlnI BsaBI BsaHI
BsaI BseAI BsiEI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrGI BstBI ClaI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoNI FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KspI MamI MfeI MluI MroI
MunI NarI NheI NotI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI SacII
SalI SapI ScaI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspBI SspI SwaI XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 167 GGTACC G^GTACC
AccI 1373 GTATAC GT^[AC][GT]AC
Asp718I 167 GGTACC G^GTACC
AspHI 971 GAGCTC G[AT]GC[AT]^C
AspI 577 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1473 GGATCC G^GATCC
BbvCI 337 CCTCAGC CCTCAGC(-5/-2)
BglII 1542 AGATCT A^GATCT
BlpI 860 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 860 GCTCAGC GC^T[ACGT]AGC
BsaAI 677 TACGTG [CT]AC^GT[GA]
BsgI 1028 GTGCAG GTGCAG(16/14)
BsiHKAI 971 GAGCTC G[AT]GC[AT]^C
BsiWI 188 CGTACG C^GTACG
BsmBI 1600 GAGACG CGTCTC(1/5)
BspHI 1038 TCATGA T^CATGA
BsrFI 885 GCCGGC [GA]^CCGG[CT]
BssHII 106 GCGCGC G^CGCGC
BssSI 878 CACGAG CACGAG(-5/-1)
Bst1107I 1373 GTATAC GTA^TAC
BstZ17I 1373 GTATAC GTA^TAC
CelII 860 GCTCAGC GC^T[ACGT]AGC
Cfr10I 885 GCCGGC [GA]^CCGG[CT]
CpoI 48 CGGACCG CG^G[AT]CCG
EarI 569 GAAGAG CTCTTC(1/4)
EcoRV 2027 GATATC GAT^ATC
KpnI 167 GGTACC GGTAC^C
Ksp632I 569 GAAGAG CTCTTC(1/4)
NaeI 885 GCCGGC GCC^GGC
NgoMIV 885 GCCGGC G^CCGGC
NruI 214 TCGCGA TCG^CGA
PflFI 577 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1463 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 440 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1038 TCATGA T^CATGA
RsrII 48 CGGACCG CG^G[AT]CCG
SacI 971 GAGCTC GAGCT^C
SbfI 1489 CCTGCAGG CCTGCA^GG
SfaNI 2114 GCATC GCATC(5/9)
SfiI 1632 GGCCAAGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1489 CCTGCAGG CCTGCA^GG
StuI 1518 AGGCCT AGG^CCT
TfiI 251 GAATC G^A[AT]TC
Tth111I 577 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1463 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 1665 GAATTC [GA]^AATT[CT]
1980 GAATTC
AocI 385 CCTGAGG CC^T[ACGT]AGG
1250 CCTGAGG
Aor51HI 1146 AGCGCT AGC^GCT
1479 AGCGCT
ApoI 1665 GAATTC [GA]^AATT[CT]
1980 GAATTC
AvaI 740 CCCGAG C^[CT]CG[GA]G
1275 CCCGAG
BalI 714 TGGCCA TGG^CCA
1755 TGGCCA
BbsI 747 GTCTTC GAAGAC(2/6)
1295 GTCTTC
BciVI 78 GGATAC GTATCC(6/5)
1096 GGATAC
BcnI 46 CCCGG CC^[GC]GG
234 CCGGG
BglI 716 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1633 GCCAAGAGGGC
BpuAI 747 GTCTTC GAAGAC(2/6)
1295 GTCTTC
BsaWI 75 ACCGGA [AT]^CCGG[AT]
302 TCCGGT
BsoBI 740 CCCGAG C^[CT]CG[GA]G
1275 CCCGAG
BsrDI 262 CATTGC GCAATG(2/0)
1575 CATTGC
BstAPI 991 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1499 GCAGACCCTGC
BstEII 1240 GGTCACC G^GT[ACGT]ACC
1427 GGTGACC
BstPI 1240 GGTCACC G^GT[ACGT]ACC
1427 GGTGACC
Bsu36I 385 CCTGAGG CC^T[ACGT]AGG
1250 CCTGAGG
DsaI 720 CCATGG C^C[GA][CT]GG
1908 CCATGG
Eco47III 1146 AGCGCT AGC^GCT
1479 AGCGCT
Eco81I 385 CCTGAGG CC^T[ACGT]AGG
1250 CCTGAGG
EcoO65I 1240 GGTCACC G^GT[ACGT]ACC
1427 GGTGACC
EcoRI 1665 GAATTC G^AATTC
1980 GAATTC
EcoT22I 2108 ATGCAT ATGCA^T
2112 ATGCAT
MaeII 187 ACGT A^CGT
678 ACGT
MluNI 714 TGGCCA TGG^CCA
1755 TGGCCA
MscI 714 TGGCCA TGG^CCA
1755 TGGCCA
MseI 1389 TTAA T^TAA
2199 TTAA
NciI 46 CCCGG CC^[GC]GG
234 CCGGG
NcoI 720 CCATGG C^CATGG
1908 CCATGG
NdeI 459 CATATG CA^TATG
893 CATATG
NsiI 2108 ATGCAT ATGCA^T
2112 ATGCAT
PpuMI 225 AGGTCCT [GA]G^G[AT]CC[CT]
735 GGGTCCC
SmlI 1058 CTCAAG C^T[CT][GA]AG
1070 CTCAAG
Tru9I 1389 TTAA T^TAA
2199 TTAA
XcmI 458 CCATATGAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1743 CCAAAGGGGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AvaII BanI BanII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
SphI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII