##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
Acc65I AccII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AviII BamHI BbvCI BcgI
BclI BfrI BglI BsiWI BsmI
BspDI BspLU11I BspMI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KpnI KspI
MfeI MluI MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI Psp1406I
PstI PvuI RsrII SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
StuI Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 481 TCCGGA T^CCGGA
AseI 1473 ATTAAT AT^TAAT
AsnI 1473 ATTAAT AT^TAAT
AspI 1891 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1068 CCCGAG C^[CT]CG[GA]G
AvrII 1334 CCTAGG C^CTAGG
BbeI 1803 GGCGCC GGCGC^C
BbrPI 1842 CACGTG CAC^GTG
BglII 1183 AGATCT A^GATCT
BlnI 1334 CCTAGG C^CTAGG
BsaAI 1842 CACGTG [CT]AC^GT[GA]
BsaWI 481 TCCGGA [AT]^CCGG[AT]
BseAI 481 TCCGGA T^CCGGA
BsgI 1718 CTGCAC GTGCAG(16/14)
BsiEI 64 CGGTCG CG[GA][CT]^CG
BsmBI 1453 GAGACG CGTCTC(1/5)
BsoBI 1068 CCCGAG C^[CT]CG[GA]G
BspEI 481 TCCGGA T^CCGGA
BspHI 421 TCATGA T^CATGA
EcoNI 51 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 1803 GGCGCC G^GCGCC
MroI 481 TCCGGA T^CCGGA
MslI 456 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1803 GGCGCC GG^CGCC
NcoI 1878 CCATGG C^CATGG
PflFI 1891 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1842 CACGTG CAC^GTG
PmlI 1842 CACGTG CAC^GTG
PshBI 1473 ATTAAT AT^TAAT
PvuII 2173 CAGCTG CAG^CTG
RcaI 421 TCATGA T^CATGA
SacI 1168 GAGCTC GAGCT^C
SapI 1595 GAAGAGC GCTCTTC(1/4)
SexAI 1892 ACCAGGT A^CC[AT]GGT
SfoI 1803 GGCGCC GGC^GCC
SpeI 840 ACTAGT A^CTAGT
SwaI 850 ATTTAAAT ATTT^AAAT
TfiI 234 GAATC G^A[AT]TC
Tth111I 1891 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1409 CCACCAGTCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 1455 GACGTC GACGT^C
1830 GACGTC
AccI 412 GTCTAC GT^[AC][GT]AC
836 GTCTAC
AspHI 1168 GAGCTC G[AT]GC[AT]^C
1815 GAGCAC
BbsI 566 GAAGAC GAAGAC(2/6)
1485 GAAGAC
BlpI 969 GCTCAGC GC^T[ACGT]AGC
1041 GCTGAGC
Bpu1102I 969 GCTCAGC GC^T[ACGT]AGC
1041 GCTGAGC
BpuAI 566 GAAGAC GAAGAC(2/6)
1485 GAAGAC
BsaBI 555 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1020 GATGGCTATC
BsaI 641 GAGACC GGTCTC(1/5)
1114 GAGACC
BseRI 127 CTCCTC GAGGAG(10/8)
892 GAGGAG
BsiHKAI 1168 GAGCTC G[AT]GC[AT]^C
1815 GAGCAC
Bsp1407I 363 TGTACA T^GTACA
1708 TGTACA
BsrBI 124 CCGCTC CCGCTC(-3/-3)
61 GAGCGG
BsrFI 104 GCCGGT [GA]^CCGG[CT]
1195 ACCGGC
BsrGI 363 TGTACA T^GTACA
1708 TGTACA
BstAPI 445 GCAACGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2162 GCATACCCTGC
BstXI 455 CCATCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1412 CCAGTCCATTGG
CelII 969 GCTCAGC GC^T[ACGT]AGC
1041 GCTGAGC
Cfr10I 104 GCCGGT [GA]^CCGG[CT]
1195 ACCGGC
DraI 851 TTTAAA TTT^AAA
2240 TTTAAA
DrdI 533 GACATGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1496 GACAGCCAAGTC
EcoT14I 1334 CCTAGG C^C[AT][AT]GG
1878 CCATGG
HaeII 973 AGCGCC [GA]GCGC^[CT]
1803 GGCGCC
MamI 555 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1020 GATGGCTATC
PleI 1610 GACTC GAGTC(4/5)
2118 GACTC
SfaNI 1292 GATGC GCATC(5/9)
1982 GATGC
SfcI 431 CTACAG C^T[GA][CT]AG
1121 CTACAG
SspBI 363 TGTACA T^GTACA
1708 TGTACA
SspI 1628 AATATT AAT^ATT
2216 AATATT
StyI 1334 CCTAGG C^C[AT][AT]GG
1878 CCATGG
TaqI 208 TCGA T^CGA
1166 TCGA
TthHB8I 208 TCGA T^CGA
1166 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AvaII BalI
BanI BanII BbvI BciVI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PpuMI RsaI
Sau3AI Sau96I ScrFI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII