##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BamHI BanI BbeI BciVI
BclI BfaI BfrI BlnI BsaBI
BsaHI BsaWI BseAI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstAPI
BstEII BstPI BstZ17I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT14I FbaI FseI
FspI HaeII Hin1I KasI KpnI
KspI MaeI MamI MluI MroI
NarI NcoI NdeI NheI NotI
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI StyI
SwaI XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccII 2174 CGCG CG^CG
AlwI 2058 GGATC GGATC(4/5)
ApaI 751 GGGCCC GGGCC^C
Asp700I 804 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2271 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2090 TGGCCA TGG^CCA
BbrPI 1846 CACGTG CAC^GTG
BbsI 1654 GAAGAC GAAGAC(2/6)
BbvCI 1750 GCTGAGG CCTCAGC(-5/-2)
BcgI 2235 CGACACCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2092 GCCAACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 784 AGATCT A^GATCT
BlpI 1102 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1102 GCTGAGC GC^T[ACGT]AGC
BpuAI 1654 GAAGAC GAAGAC(2/6)
BsaAI 1846 CACGTG [CT]AC^GT[GA]
BsaI 2080 GAGACC GGTCTC(1/5)
BsgI 2243 CTGCAC GTGCAG(16/14)
BsiEI 69 CGGTCG CG[GA][CT]^CG
BsmBI 2231 GAGACG CGTCTC(1/5)
BspMI 42 GCAGGT ACCTGC(4/8)
BsrFI 38 GCCGGC [GA]^CCGG[CT]
BstUI 2174 CGCG CG^CG
BstXI 2031 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 784 AGATCT [GA]^GATC[CT]
CelII 1102 GCTGAGC GC^T[ACGT]AGC
CfoI 2149 GCGC GCG^C
Cfr10I 38 GCCGGC [GA]^CCGG[CT]
DraI 1983 TTTAAA TTT^AAA
EaeI 2090 TGGCCA [CT]^GGCC[GA]
EcoT22I 1340 ATGCAT ATGCA^T
FauI 35 CCCGC CCCGC(4/6)
HhaI 2149 GCGC GCG^C
HinP1I 2149 GCGC G^CGC
HincII 825 GTTAAC GT[CT]^[GA]AC
HindII 825 GTTAAC GT[CT]^[GA]AC
HindIII 1318 AAGCTT A^AGCTT
HpaI 825 GTTAAC GTT^AAC
MfeI 1352 CAATTG C^AATTG
MflI 784 AGATCT [GA]^GATC[CT]
MluNI 2090 TGGCCA TGG^CCA
MscI 2090 TGGCCA TGG^CCA
MunI 1352 CAATTG C^AATTG
MvnI 2174 CGCG CG^CG
NaeI 38 GCCGGC GCC^GGC
NgoMIV 38 GCCGGC G^CCGGC
NruI 2173 TCGCGA TCG^CGA
NsiI 1340 ATGCAT ATGCA^T
PflFI 2271 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2205 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1846 CACGTG CAC^GTG
PmlI 1846 CACGTG CAC^GTG
SacI 289 GAGCTC GAGCT^C
SapI 478 GAAGAGC GCTCTTC(1/4)
Tth111I 2271 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2205 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 784 AGATCT [GA]^GATC[CT]
XmnI 804 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 1155 AAATTC [GA]^AATT[CT]
1586 AAATTC
AflIII 251 ACACGT A^C[GA][CT]GT
1832 ACACGT
AlwNI 838 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1758 CAGGGACTG
AocI 852 CCTAAGG CC^T[ACGT]AGG
1524 CCTCAGG
ApoI 1155 AAATTC [GA]^AATT[CT]
1586 AAATTC
AspHI 289 GAGCTC G[AT]GC[AT]^C
975 GAGCAC
BsiHKAI 289 GAGCTC G[AT]GC[AT]^C
975 GAGCAC
BspHI 456 TCATGA T^CATGA
1590 TCATGA
BssSI 756 CACGAG CACGAG(-5/-1)
777 CACGAG
BstBI 811 TTCGAA TT^CGAA
1651 TTCGAA
Bsu36I 852 CCTAAGG CC^T[ACGT]AGG
1524 CCTCAGG
DpnII 785 GATC ^GATC
2059 GATC
Eco81I 852 CCTAAGG CC^T[ACGT]AGG
1524 CCTCAGG
HgaI 58 GCGTC GACGC(5/10)
438 GCGTC
MboI 785 GATC ^GATC
2059 GATC
NdeII 785 GATC ^GATC
2059 GATC
NspI 1235 GCATGC [GA]CATG^[CT]
2151 GCATGC
NspV 811 TTCGAA TT^CGAA
1651 TTCGAA
RcaI 456 TCATGA T^CATGA
1590 TCATGA
Sau3AI 785 GATC ^GATC
2059 GATC
SfuI 811 TTCGAA TT^CGAA
1651 TTCGAA
SphI 1235 GCATGC GCATG^C
2151 GCATGC
SspI 1780 AATATT AAT^ATT
1959 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PleI
PpuMI PstI PvuII Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I