##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BsaAI BsaBI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I CpoI DraIII
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MamI MfeI MluI MroI MunI
NcoI NdeI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I Van91I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AcyI 249 GGCGCC G[GA]^CG[CT]C
AgeI 513 ACCGGT A^CCGGT
ApaI 1423 GGGCCC GGGCC^C
AspHI 94 GAGCTC G[AT]GC[AT]^C
AvrII 1701 CCTAGG C^CTAGG
BalI 1319 TGGCCA TGG^CCA
BbeI 249 GGCGCC GGCGC^C
BglII 1924 AGATCT A^GATCT
BlnI 1701 CCTAGG C^CTAGG
BlpI 216 GCTCAGC GC^T[ACGT]AGC
BpmI 162 CTCCAG CTGGAG(16/14)
Bpu1102I 216 GCTCAGC GC^T[ACGT]AGC
BsaHI 249 GGCGCC G[GA]^CG[CT]C
BsaI 44 GAGACC GGTCTC(1/5)
BsiHKAI 94 GAGCTC G[AT]GC[AT]^C
BsmFI 2066 GTCCC GGGAC(10/14)
BspDI 1686 ATCGAT AT^CGAT
BstAPI 207 GCAGGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 506 GGTCACC G^GT[ACGT]ACC
BstPI 506 GGTCACC G^GT[ACGT]ACC
BstXI 532 CCAAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 216 GCTCAGC GC^T[ACGT]AGC
ClaI 1686 ATCGAT AT^CGAT
DrdI 865 GACAATGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 240 CCTTTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 506 GGTCACC G^GT[ACGT]ACC
EcoRI 290 GAATTC G^AATTC
HgaI 2064 GCGTC GACGC(5/10)
Hin1I 249 GGCGCC G[GA]^CG[CT]C
KasI 249 GGCGCC G^GCGCC
MaeII 297 ACGT A^CGT
MluNI 1319 TGGCCA TGG^CCA
MscI 1319 TGGCCA TGG^CCA
MspA1I 1512 CAGCTG C[AC]G^C[GT]G
NarI 249 GGCGCC GG^CGCC
NheI 563 GCTAGC G^CTAGC
NspI 1934 GCATGT [GA]CATG^[CT]
PinAI 513 ACCGGT A^CCGGT
PpuMI 552 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 1883 CTGCAG CTGCA^G
PvuII 1512 CAGCTG CAG^CTG
SacI 94 GAGCTC GAGCT^C
ScaI 1972 AGTACT AGT^ACT
SfcI 1883 CTGCAG C^T[GA][CT]AG
SfoI 249 GGCGCC GGC^GCC
SgrAI 512 CACCGGTG C[GA]^CCGG[CT]G
StuI 331 AGGCCT AGG^CCT
TfiI 1001 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwI 198 GATCC GGATC(4/5)
1568 GGATC
BsaWI 513 ACCGGT [AT]^CCGG[AT]
1602 ACCGGA
Bsp1407I 1742 TGTACA T^GTACA
2011 TGTACA
BsrGI 1742 TGTACA T^GTACA
2011 TGTACA
EcoT14I 328 CCAAGG C^C[AT][AT]GG
1701 CCTAGG
HaeII 249 GGCGCC [GA]GCGC^[CT]
499 AGCGCC
MslI 371 CATGATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1935 CATGTGTGTG
SmaI 149 CCCGGG CCC^GGG
1988 CCCGGG
SmlI 522 CTCAAG C^T[CT][GA]AG
1281 CTTGAG
SspBI 1742 TGTACA T^GTACA
2011 TGTACA
StyI 328 CCAAGG C^C[AT][AT]GG
1701 CCTAGG
TaqI 1687 TCGA T^CGA
1889 TCGA
TthHB8I 1687 TCGA T^CGA
1889 TCGA
XmaCI 149 CCCGGG C^CCGGG
1988 CCCGGG
XmaI 149 CCCGGG C^CCGGG
1988 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII