##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AgeI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspI AvrII BalI BamHI BanII
BbeI BbvCI BglII BlnI BlpI
Bpu1102I BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstUI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FseI HaeIII
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
StuI SwaI Tth111I Van91I XhoI
XhoII
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AcyI 53 GGCGTC G[GA]^CG[CT]C
AfaI 1162 GTAC GT^AC
AflIII 1631 ACGTGT A^C[GA][CT]GT
AhdI 116 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 91 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 932 ATTAAT AT^TAAT
AsnI 932 ATTAAT AT^TAAT
AspEI 116 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 811 CCCGGG C^[CT]CG[GA]G
AviII 1241 TGCGCA TGC^GCA
BbrPI 1666 CACGTG CAC^GTG
BcgI 1087 GCAAACTTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 782 GGATAC GTATCC(6/5)
BglI 447 GCCACGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 636 CTGGAG CTGGAG(16/14)
BsaAI 1666 CACGTG [CT]AC^GT[GA]
BsaHI 53 GGCGTC G[GA]^CG[CT]C
BsmI 1659 GCATTC GAATGC(1/-1)
BsoBI 811 CCCGGG C^[CT]CG[GA]G
BspHI 1105 TCATGA T^CATGA
BspMI 1002 GCAGGT ACCTGC(4/8)
BssSI 5 CACGAG CACGAG(-5/-1)
BstEII 236 GGTAACC G^GT[ACGT]ACC
BstF5I 414 CATCC GGATG(2/0)
BstPI 236 GGTAACC G^GT[ACGT]ACC
BstXI 184 CCAAAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 179 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 49 CCATGG C^C[GA][CT]GG
Eam1105I 116 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 102 GAAGAG CTCTTC(1/4)
EcoNI 1148 CCTTGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 179 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 236 GGTAACC G^GT[ACGT]ACC
FokI 414 CATCC GGATG(9/13)
FspI 1241 TGCGCA TGC^GCA
HaeII 467 GGCGCT [GA]GCGC^[CT]
HapII 812 CCGG C^CGG
HgaI 54 GCGTC GACGC(5/10)
Hin1I 53 GGCGTC G[GA]^CG[CT]C
HindIII 1074 AAGCTT A^AGCTT
HpaI 75 GTTAAC GTT^AAC
HpaII 812 CCGG C^CGG
Ksp632I 102 GAAGAG CTCTTC(1/4)
MslI 24 CACTTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1570 CAGCTG C[AC]G^C[GT]G
MspI 812 CCGG C^CGG
NcoI 49 CCATGG C^CATGG
PmaCI 1666 CACGTG CAC^GTG
PmlI 1666 CACGTG CAC^GTG
PpuMI 179 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 932 ATTAAT AT^TAAT
PstI 481 CTGCAG CTGCA^G
PvuII 1570 CAGCTG CAG^CTG
RcaI 1105 TCATGA T^CATGA
RsaI 1162 GTAC GT^AC
SexAI 1429 ACCTGGT A^CC[AT]GGT
SmaI 811 CCCGGG CCC^GGG
SphI 989 GCATGC GCATG^C
SspI 531 AATATT AAT^ATT
XbaI 1260 TCTAGA T^CTAGA
XmaCI 811 CCCGGG C^CCGGG
XmaI 811 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 897 CTTAAG C^TTAAG
1767 CTTAAG
AspHI 2 GAGCAC G[AT]GC[AT]^C
170 GAGCAC
AvaII 180 GGACC G^G[AT]CC
1684 GGACC
BbsI 1116 GAAGAC GAAGAC(2/6)
1729 GAAGAC
BcnI 811 CCCGG CC^[GC]GG
812 CCGGG
BfrI 897 CTTAAG C^TTAAG
1767 CTTAAG
BpuAI 1116 GAAGAC GAAGAC(2/6)
1729 GAAGAC
BseRI 39 CTCCTC GAGGAG(10/8)
1276 GAGGAG
BsgI 1423 GTGCAG GTGCAG(16/14)
1567 GTGCAG
BsiHKAI 2 GAGCAC G[AT]GC[AT]^C
170 GAGCAC
BsiYI 1061 CCCAGAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1148 CCTTGGTGAGG
BslI 1061 CCCAGAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1148 CCTTGGTGAGG
BsmAI 114 GAGAC GTCTC(1/5)
157 GAGAC
BsrDI 1216 CATTGC GCAATG(2/0)
1353 GCAATG
BstAPI 834 GCAAAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
993 GCAGATTTTGC
CfoI 468 GCGC GCG^C
1242 GCGC
Cfr13I 180 GGACC G^G[ACGT]CC
1684 GGACC
FauI 432 GCGGG CCCGC(4/6)
537 GCGGG
HhaI 468 GCGC GCG^C
1242 GCGC
HinP1I 468 GCGC G^CGC
1242 GCGC
NciI 811 CCCGG CC^[GC]GG
812 CCGGG
NspI 854 ACATGC [GA]CATG^[CT]
989 GCATGC
Sau96I 180 GGACC G^G[ACGT]CC
1684 GGACC
SfaNI 222 GATGC GCATC(5/9)
1224 GATGC
SfcI 481 CTGCAG C^T[GA][CT]AG
598 CTGTAG
SmlI 897 CTTAAG C^T[CT][GA]AG
1767 CTTAAG
TaqI 1096 TCGA T^CGA
1675 TCGA
Tsp45I 1026 GTCAC ^GT[GC]AC
1311 GTGAC
TthHB8I 1096 TCGA T^CGA
1675 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AluI ApoI Asp700I
BanI BbvI BclI BfaI BmyI
BsaJI BsmFI Bsp1286I BsrI BssKI
BstNI Cac8I DdeI DpnII EcoRII
EcoT14I FbaI Fnu4HI HincII HindII
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI ScrFI StyI TfiI
Tru9I TseI Tsp509I TspRI XcmI
XmnI