##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI AocI AscI AspEI
AspI AviII AvrII BbrPI BbsI
BbvCI BciVI BclI BfrI BglII
BlnI BpuAI BsaBI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraIII
DrdI Eam1105I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SapI SbfI ScaI SexAI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 629 GGTACC G^GTACC
AccI 917 GTCGAC GT^[AC][GT]AC
AcsI 2398 AAATTT [GA]^AATT[CT]
AcyI 32 GGCGCC G[GA]^CG[CT]C
Alw44I 636 GTGCAC G^TGCAC
Aor51HI 85 AGCGCT AGC^GCT
ApaLI 636 GTGCAC G^TGCAC
ApoI 2398 AAATTT [GA]^AATT[CT]
AseI 2280 ATTAAT AT^TAAT
AsnI 2280 ATTAAT AT^TAAT
Asp700I 1580 GAACATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 629 GGTACC G^GTACC
BamHI 2055 GGATCC G^GATCC
BbeI 32 GGCGCC GGCGC^C
BlpI 1445 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1445 GCTCAGC GC^T[ACGT]AGC
BsaAI 1250 TACGTG [CT]AC^GT[GA]
BsaHI 32 GGCGCC G[GA]^CG[CT]C
BsgI 1455 CTGCAC GTGCAG(16/14)
BsrDI 570 CATTGC GCAATG(2/0)
BsrFI 393 GCCGGC [GA]^CCGG[CT]
CelII 1445 GCTCAGC GC^T[ACGT]AGC
Cfr10I 393 GCCGGC [GA]^CCGG[CT]
DraI 2335 TTTAAA TTT^AAA
EarI 886 CTCTTC CTCTTC(1/4)
Eco47III 85 AGCGCT AGC^GCT
EcoNI 367 CCTCATCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 32 GGCGCC G[GA]^CG[CT]C
HincII 917 GTCGAC GT[CT]^[GA]AC
HindII 917 GTCGAC GT[CT]^[GA]AC
KasI 32 GGCGCC G^GCGCC
KpnI 629 GGTACC GGTAC^C
Ksp632I 886 CTCTTC CTCTTC(1/4)
NaeI 393 GCCGGC GCC^GGC
NarI 32 GGCGCC GG^CGCC
NgoMIV 393 GCCGGC G^CCGGC
NspI 2146 GCATGT [GA]CATG^[CT]
PpuMI 829 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 2280 ATTAAT AT^TAAT
SacI 660 GAGCTC GAGCT^C
SalI 917 GTCGAC G^TCGAC
SfiI 320 GGCCCTTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 32 GGCGCC GGC^GCC
SspI 2386 AATATT AAT^ATT
TfiI 1600 GAATC G^A[AT]TC
XmnI 1580 GAACATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
BsmBI 236 CGTCTC CGTCTC(1/5)
475 GAGACG
BspMI 1453 ACCTGC ACCTGC(4/8)
716 GCAGGT
BssSI 472 CACGAG CACGAG(-5/-1)
2115 CACGAG
BstXI 1650 CCACTTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2064 CCAAGGCACTGG
BstYI 420 AGATCC [GA]^GATC[CT]
2055 GGATCC
MflI 420 AGATCC [GA]^GATC[CT]
2055 GGATCC
NcoI 39 CCATGG C^CATGG
967 CCATGG
PleI 1466 GAGTC GAGTC(4/5)
1867 GAGTC
SmlI 1939 CTTGAG C^T[CT][GA]AG
2341 CTTGAG
XhoII 420 AGATCC [GA]^GATC[CT]
2055 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvI BcgI
BcnI BfaI BglI BmyI BpmI
BsaI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI KspI MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I