##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccIII AcsI AflII AgeI
AlwI ApoI AscI AseI AsnI
Asp700I AspI AviII AvrII BamHI
BbrPI BbvCI BciVI BclI BfaI
BfrI BlnI BsaBI BsaWI BseAI
BsiWI BsmBI BsmI Bsp1407I BspEI
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I CpoI DraI
DraIII EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI MaeI MamI MfeI MluI
MroI MunI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SalI SbfI
ScaI SfuI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tsp509I
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1587 GGTACC G^GTACC
AclI 1664 AACGTT AA^CGTT
Alw44I 1166 GTGCAC G^TGCAC
Aor51HI 1818 AGCGCT AGC^GCT
ApaLI 1166 GTGCAC G^TGCAC
Asp718I 1587 GGTACC G^GTACC
AvaI 155 CCCGGG C^[CT]CG[GA]G
BlpI 1814 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1814 GCTGAGC GC^T[ACGT]AGC
BsgI 1772 CTGCAC GTGCAG(16/14)
BsoBI 155 CCCGGG C^[CT]CG[GA]G
BspDI 1421 ATCGAT AT^CGAT
BspHI 426 TCATGA T^CATGA
BspLU11I 593 ACATGT A^CATGT
BsrDI 1570 CATTGC GCAATG(2/0)
BstEII 784 GGTGACC G^GT[ACGT]ACC
BstPI 784 GGTGACC G^GT[ACGT]ACC
BstXI 1035 CCATTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1814 GCTGAGC GC^T[ACGT]AGC
ClaI 1421 ATCGAT AT^CGAT
EarI 91 GAAGAG CTCTTC(1/4)
Eco47III 1818 AGCGCT AGC^GCT
EcoNI 2070 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 784 GGTGACC G^GT[ACGT]ACC
KpnI 1587 GGTACC GGTAC^C
Ksp632I 91 GAAGAG CTCTTC(1/4)
KspI 1220 CCGCGG CCGC^GG
MseI 2128 TTAA T^TAA
NaeI 434 GCCGGC GCC^GGC
NgoMIV 434 GCCGGC G^CCGGC
NotI 213 GCGGCCGC GC^GGCCGC
NspI 593 ACATGT [GA]CATG^[CT]
PleI 1187 GAGTC GAGTC(4/5)
Psp1406I 1664 AACGTT AA^CGTT
PstI 1152 CTGCAG CTGCA^G
PvuII 1463 CAGCTG CAG^CTG
RcaI 426 TCATGA T^CATGA
SacI 1872 GAGCTC GAGCT^C
SacII 1220 CCGCGG CCGC^GG
SapI 91 GAAGAGC GCTCTTC(1/4)
SexAI 555 ACCTGGT A^CC[AT]GGT
SfiI 188 GGCCCCGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 6 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 155 CCCGGG CCC^GGG
TfiI 387 GAATC G^A[AT]TC
Tru9I 2128 TTAA T^TAA
XmaCI 155 CCCGGG C^CCGGG
XmaI 155 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccI 818 GTCTAC GT^[AC][GT]AC
1688 GTCTAC
AcyI 42 GGCGCC G[GA]^CG[CT]C
932 GGCGCC
AhdI 695 GACCGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
787 GACCCTTTGTC
AlwNI 1826 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
2002 CAGCCCCTG
AocI 989 CCTCAGG CC^T[ACGT]AGG
1792 CCTCAGG
AspEI 695 GACCGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
787 GACCCTTTGTC
BalI 598 TGGCCA TGG^CCA
666 TGGCCA
BbeI 42 GGCGCC GGCGC^C
932 GGCGCC
BcgI 1926 GCAGCAAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1929 GCAAGGTCGTCG
BglI 189 GCCCCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
659 GCCAGCCTGGC
BpmI 2030 CTCCAG CTGGAG(16/14)
755 CTGGAG
BsaAI 887 TACGTG [CT]AC^GT[GA]
1703 TACGTG
BsaHI 42 GGCGCC G[GA]^CG[CT]C
932 GGCGCC
BsaI 239 GAGACC GGTCTC(1/5)
1292 GGTCTC
BsiEI 60 CGGCCG CG[GA][CT]^CG
214 CGGCCG
BsmAI 239 GAGAC GTCTC(1/5)
1293 GTCTC
BstAPI 1159 GCAGGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2001 GCAGCCCCTGC
Bsu36I 989 CCTCAGG CC^T[ACGT]AGG
1792 CCTCAGG
DrdI 1880 GACGCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1909 GACCCCAGTGTC
EagI 60 CGGCCG C^GGCCG
214 CGGCCG
Eam1105I 695 GACCGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
787 GACCCTTTGTC
EclXI 60 CGGCCG C^GGCCG
214 CGGCCG
Eco52I 60 CGGCCG C^GGCCG
214 CGGCCG
Eco81I 989 CCTCAGG CC^T[ACGT]AGG
1792 CCTCAGG
HgaI 247 GACGC GACGC(5/10)
1880 GACGC
Hin1I 42 GGCGCC G[GA]^CG[CT]C
932 GGCGCC
HinfI 387 GAATC G^A[ACGT]TC
1187 GAGTC
KasI 42 GGCGCC G^GCGCC
932 GGCGCC
MluNI 598 TGGCCA TGG^CCA
666 TGGCCA
MscI 598 TGGCCA TGG^CCA
666 TGGCCA
NarI 42 GGCGCC GG^CGCC
932 GGCGCC
PflMI 1728 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1855 CCACCACTTGG
PpuMI 983 GGGTCCC [GA]G^G[AT]CC[CT]
1010 GGGACCC
SfcI 112 CTATAG C^T[GA][CT]AG
1152 CTGCAG
SfoI 42 GGCGCC GGC^GCC
932 GGCGCC
SmlI 736 CTTGAG C^T[CT][GA]AG
959 CTCAAG
StuI 475 AGGCCT AGG^CCT
1509 AGGCCT
Van91I 1728 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1855 CCACCACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
ApaI AspHI AvaII BanI BanII
BbsI BbvI BcnI BglII BmyI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XcmI XhoII