##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BbeI BcgI BciVI BclI
BfrI BlnI BsaBI BsaHI BsaI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MseI MunI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
SspBI SspI SwaI Tru9I Tth111I
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 638 TCCGGA T^CCGGA
AcsI 503 GAATTT [GA]^AATT[CT]
AocI 1382 CCTCAGG CC^T[ACGT]AGG
ApoI 503 GAATTT [GA]^AATT[CT]
Asp700I 678 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 990 TGGCCA TGG^CCA
BamHI 289 GGATCC G^GATCC
BbrPI 1376 CACGTG CAC^GTG
BbvCI 771 CCTCAGC CCTCAGC(-5/-2)
BfaI 74 CTAG C^TAG
BglII 1250 AGATCT A^GATCT
BseAI 638 TCCGGA T^CCGGA
BsiEI 229 CGGTCG CG[GA][CT]^CG
BsmAI 970 GAGAC GTCTC(1/5)
BspEI 638 TCCGGA T^CCGGA
BsrBI 509 CCGCTC CCGCTC(-3/-3)
BsrFI 227 GCCGGT [GA]^CCGG[CT]
BssSI 1353 CTCGTG CACGAG(-5/-1)
Bsu36I 1382 CCTCAGG CC^T[ACGT]AGG
Cfr10I 227 GCCGGT [GA]^CCGG[CT]
EaeI 990 TGGCCA [CT]^GGCC[GA]
Eco81I 1382 CCTCAGG CC^T[ACGT]AGG
MaeI 74 CTAG C^TAG
MluNI 990 TGGCCA TGG^CCA
MroI 638 TCCGGA T^CCGGA
MscI 990 TGGCCA TGG^CCA
MslI 54 CATGGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 457 CATATG CA^TATG
PleI 1317 GAGTC GAGTC(4/5)
PmaCI 1376 CACGTG CAC^GTG
PmlI 1376 CACGTG CAC^GTG
PstI 1503 CTGCAG CTGCA^G
PvuII 312 CAGCTG CAG^CTG
SbfI 1502 CCTGCAGG CCTGCA^GG
ScaI 660 AGTACT AGT^ACT
Sse8387I 1502 CCTGCAGG CCTGCA^GG
TaqI 880 TCGA T^CGA
TfiI 424 GAATC G^A[AT]TC
TthHB8I 880 TCGA T^CGA
XmnI 678 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
BanI 857 GGTGCC G^G[CT][GA]CC
1416 GGTGCC
BglI 934 GCCCGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
983 GCCTGGATGGC
BlpI 1061 GCTGAGC GC^T[ACGT]AGC
1549 GCTGAGC
Bpu1102I 1061 GCTGAGC GC^T[ACGT]AGC
1549 GCTGAGC
BsaAI 1047 TACGTG [CT]AC^GT[GA]
1376 CACGTG
BsaWI 553 ACCGGA [AT]^CCGG[AT]
638 TCCGGA
BsgI 24 CTGCAC GTGCAG(16/14)
1446 CTGCAC
CelII 1061 GCTGAGC GC^T[ACGT]AGC
1549 GCTGAGC
EcoNI 392 CCTACGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
493 CCTGGAAGAGG
FauI 1094 GCGGG CCCGC(4/6)
1177 GCGGG
HinfI 424 GAATC G^A[ACGT]TC
1317 GAGTC
MaeII 1048 ACGT A^CGT
1377 ACGT
NcoI 53 CCATGG C^CATGG
268 CCATGG
NspI 1015 GCATGT [GA]CATG^[CT]
1055 GCATGT
PflMI 1005 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1118 CCAGCGGCTGG
SfaNI 402 GATGC GCATC(5/9)
373 GCATC
SfcI 751 CTGTAG C^T[GA][CT]AG
1503 CTGCAG
SmaI 465 CCCGGG CCC^GGG
1405 CCCGGG
StuI 433 AGGCCT AGG^CCT
1495 AGGCCT
Tsp45I 885 GTGAC ^GT[GC]AC
996 GTCAC
Tsp509I 5 AATT ^AATT
504 AATT
Van91I 1005 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1118 CCAGCGGCTGG
XmaCI 465 CCCGGG C^CCGGG
1405 CCCGGG
XmaI 465 CCCGGG C^CCGGG
1405 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AvaI AvaII BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PpuMI RsaI Sau3AI
Sau96I ScrFI StyI TseI TspRI
XhoII