##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AvrII
BamHI BanII BbrPI BbvCI BcgI
BciVI BclI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspDI
BspEI BspLU11I BspMI BsrBI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HpaI KpnI KspI MaeII MamI
MluI MroI MspA1I MvnI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Van91I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 632 GTAGAC GT^[AC][GT]AC
AcsI 817 GAATTT [GA]^AATT[CT]
AflII 1012 CTTAAG C^TTAAG
AlwI 97 GATCC GGATC(4/5)
ApoI 817 GAATTT [GA]^AATT[CT]
Asp700I 409 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 549 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 311 TGCGCA TGC^GCA
BbeI 237 GGCGCC GGCGC^C
BfrI 1012 CTTAAG C^TTAAG
BsaI 305 GGTCTC GGTCTC(1/5)
Bsp1407I 848 TGTACA T^GTACA
BspHI 809 TCATGA T^CATGA
BsrDI 1100 GCAATG GCAATG(2/0)
BsrFI 216 GCCGGC [GA]^CCGG[CT]
BsrGI 848 TGTACA T^GTACA
BssSI 182 CACGAG CACGAG(-5/-1)
BstYI 96 AGATCC [GA]^GATC[CT]
Cfr10I 216 GCCGGC [GA]^CCGG[CT]
DraI 1094 TTTAAA TTT^AAA
DsaI 559 CCATGG C^C[GA][CT]GG
EarI 52 GAAGAG CTCTTC(1/4)
EcoNI 1000 CCTTCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 311 TGCGCA TGC^GCA
HindIII 1015 AAGCTT A^AGCTT
KasI 237 GGCGCC G^GCGCC
Ksp632I 52 GAAGAG CTCTTC(1/4)
MfeI 893 CAATTG C^AATTG
MflI 96 AGATCC [GA]^GATC[CT]
MunI 893 CAATTG C^AATTG
NaeI 216 GCCGGC GCC^GGC
NarI 237 GGCGCC GG^CGCC
NcoI 559 CCATGG C^CATGG
NgoMIV 216 GCCGGC G^CCGGC
NspI 116 ACATGC [GA]CATG^[CT]
PflFI 549 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 513 GAGTC GAGTC(4/5)
PstI 92 CTGCAG CTGCA^G
RcaI 809 TCATGA T^CATGA
SapI 52 GAAGAGC GCTCTTC(1/4)
ScaI 174 AGTACT AGT^ACT
SfoI 237 GGCGCC GGC^GCC
SmaI 518 CCCGGG CCC^GGG
SspBI 848 TGTACA T^GTACA
StuI 1008 AGGCCT AGG^CCT
Tth111I 549 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 130 CCAGCAGAACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 96 AGATCC [GA]^GATC[CT]
XmaCI 518 CCCGGG C^CCGGG
XmaI 518 CCCGGG C^CCGGG
XmnI 409 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 237 GGCGCC G[GA]^CG[CT]C
549 GACGCC
AfaI 175 GTAC GT^AC
849 GTAC
AlwNI 679 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
795 CAGGCGCTG
AvaI 510 CCCGAG C^[CT]CG[GA]G
518 CCCGGG
BalI 732 TGGCCA TGG^CCA
976 TGGCCA
BanI 11 GGCACC G^G[CT][GA]CC
237 GGCGCC
BbsI 546 GAAGAC GAAGAC(2/6)
572 GTCTTC
BfaI 882 CTAG C^TAG
1212 CTAG
BpuAI 546 GAAGAC GAAGAC(2/6)
572 GTCTTC
BsaHI 237 GGCGCC G[GA]^CG[CT]C
549 GACGCC
BseRI 437 CTCCTC GAGGAG(10/8)
1145 GAGGAG
BsmBI 554 CGTCTC CGTCTC(1/5)
1043 GAGACG
BsmFI 144 GGGAC GGGAC(10/14)
515 GTCCC
BsoBI 510 CCCGAG C^[CT]CG[GA]G
518 CCCGGG
DpnII 97 GATC ^GATC
490 GATC
HaeII 237 GGCGCC [GA]GCGC^[CT]
797 GGCGCT
Hin1I 237 GGCGCC G[GA]^CG[CT]C
549 GACGCC
MaeI 882 CTAG C^TAG
1212 CTAG
MboI 97 GATC ^GATC
490 GATC
MluNI 732 TGGCCA TGG^CCA
976 TGGCCA
MscI 732 TGGCCA TGG^CCA
976 TGGCCA
MslI 413 CATTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
661 CATCCAAGTG
NdeII 97 GATC ^GATC
490 GATC
RsaI 175 GTAC GT^AC
849 GTAC
Sau3AI 97 GATC ^GATC
490 GATC
SfcI 92 CTGCAG C^T[GA][CT]AG
828 CTACAG
SmlI 716 CTTGAG C^T[CT][GA]AG
1012 CTTAAG
TaqI 45 TCGA T^CGA
168 TCGA
TfiI 331 GATTC G^A[AT]TC
1111 GAATC
TthHB8I 45 TCGA T^CGA
168 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BsaJI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI EaeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboII MnlI
MseI MspI MvaI MwoI NciI
NlaIII NlaIV Sau96I ScrFI SfaNI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI