##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI Asp700I AspEI AspI
AviII AvrII BamHI BbrPI BbsI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsgI BsiEI BsiWI
BsmBI BspDI BspHI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KspI MamI MfeI MflI MspA1I
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I Van91I
XbaI XcmI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 699 GGTACC G^GTACC
AccI 510 GTCTAC GT^[AC][GT]AC
AlwNI 114 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1000 GGGCCC GGGCC^C
AseI 1567 ATTAAT AT^TAAT
AsnI 1567 ATTAAT AT^TAAT
Asp718I 699 GGTACC G^GTACC
BbeI 1198 GGCGCC GGCGC^C
BbvCI 32 GCTGAGG CCTCAGC(-5/-2)
BfaI 681 CTAG C^TAG
BglI 671 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmI 734 GCATTC GAATGC(1/-1)
Bsp1407I 1345 TGTACA T^GTACA
BspLU11I 1342 ACATGT A^CATGT
BspMI 169 ACCTGC ACCTGC(4/8)
BsrDI 227 CATTGC GCAATG(2/0)
BsrFI 889 ACCGGC [GA]^CCGG[CT]
BsrGI 1345 TGTACA T^GTACA
BsrI 113 CCAGT ACTGG(1/-1)
BstXI 127 CCAACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 889 ACCGGC [GA]^CCGG[CT]
DraI 1555 TTTAAA TTT^AAA
DsaI 536 CCGTGG C^C[GA][CT]GG
HphI 1074 GGTGA GGTGA(8/7)
KasI 1198 GGCGCC G^GCGCC
KpnI 699 GGTACC GGTAC^C
MaeI 681 CTAG C^TAG
MaeII 637 ACGT A^CGT
MluI 1453 ACGCGT A^CGCGT
NarI 1198 GGCGCC GG^CGCC
NruI 911 TCGCGA TCG^CGA
PaeR7I 259 CTCGAG C^TCGAG
PshBI 1567 ATTAAT AT^TAAT
SacI 1107 GAGCTC GAGCT^C
SfcI 512 CTACAG C^T[GA][CT]AG
SfoI 1198 GGCGCC GGC^GCC
SmaI 883 CCCGGG CCC^GGG
SspBI 1345 TGTACA T^GTACA
StuI 689 AGGCCT AGG^CCT
TfiI 453 GAATC G^A[AT]TC
TspRI 1259 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 259 CTCGAG C^TCGAG
XmaCI 883 CCCGGG C^CCGGG
XmaI 883 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcyI 70 GACGCC G[GA]^CG[CT]C
1198 GGCGCC
AflIII 1342 ACATGT A^C[GA][CT]GT
1453 ACGCGT
AspHI 1107 GAGCTC G[AT]GC[AT]^C
1236 GAGCAC
BalI 1298 TGGCCA TGG^CCA
1498 TGGCCA
BanI 699 GGTACC G^G[CT][GA]CC
1198 GGCGCC
BsaHI 70 GACGCC G[GA]^CG[CT]C
1198 GGCGCC
BsiHKAI 1107 GAGCTC G[AT]GC[AT]^C
1236 GAGCAC
BssSI 564 CACGAG CACGAG(-5/-1)
280 CTCGTG
EaeI 1298 TGGCCA [CT]^GGCC[GA]
1498 TGGCCA
Hin1I 70 GACGCC G[GA]^CG[CT]C
1198 GGCGCC
MluNI 1298 TGGCCA TGG^CCA
1498 TGGCCA
MscI 1298 TGGCCA TGG^CCA
1498 TGGCCA
MslI 594 CACGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1343 CATGTACATG
NspI 574 ACATGC [GA]CATG^[CT]
1342 ACATGT
SfaNI 264 GATGC GCATC(5/9)
405 GATGC
SmlI 259 CTCGAG C^T[CT][GA]AG
1504 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AvaI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BsaWI
BseAI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BsrBI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
ItaI Ksp632I MaeIII MboI MboII
MnlI MroI MseI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TthHB8I