##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI Asp718I AspEI AspI
AvaI AviII BanI BbeI BbrPI
BbvCI BcgI BglI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstXI
Bsu36I Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
EcoT22I FseI FspI Hin1I KasI
KpnI KspI MfeI MluI MroI
MslI MunI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AflII 535 CTTAAG C^TTAAG
Alw44I 1007 GTGCAC G^TGCAC
ApaLI 1007 GTGCAC G^TGCAC
Asp700I 732 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 552 GGACC G^G[AT]CC
AvrII 2254 CCTAGG C^CTAGG
BalI 668 TGGCCA TGG^CCA
BamHI 1524 GGATCC G^GATCC
BanII 292 GAGCTC G[GA]GC[CT]^C
BbsI 480 GAAGAC GAAGAC(2/6)
BciVI 778 GGATAC GTATCC(6/5)
BclI 762 TGATCA T^GATCA
BcnI 37 CCCGG CC^[GC]GG
BfrI 535 CTTAAG C^TTAAG
BglII 221 AGATCT A^GATCT
BlnI 2254 CCTAGG C^CTAGG
BpmI 295 CTCCAG CTGGAG(16/14)
BpuAI 480 GAAGAC GAAGAC(2/6)
BsaAI 406 CACGTA [CT]AC^GT[GA]
BsaBI 1066 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 57 GGTCTC GGTCTC(1/5)
BseRI 529 GAGGAG GAGGAG(10/8)
BsgI 685 GTGCAG GTGCAG(16/14)
BsmI 1980 GCATTC GAATGC(1/-1)
Bsp1407I 163 TGTACA T^GTACA
BspHI 476 TCATGA T^CATGA
BsrDI 773 GCAATG GCAATG(2/0)
BsrGI 163 TGTACA T^GTACA
Bst1107I 603 GTATAC GTA^TAC
BstEII 253 GGTTACC G^GT[ACGT]ACC
BstPI 253 GGTTACC G^GT[ACGT]ACC
BstZ17I 603 GTATAC GTA^TAC
Cfr13I 552 GGACC G^G[ACGT]CC
DraII 551 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 551 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 253 GGTTACC G^GT[ACGT]ACC
FbaI 762 TGATCA T^GATCA
HapII 38 CCGG C^CGG
HgaI 238 GACGC GACGC(5/10)
HindIII 1388 AAGCTT A^AGCTT
HpaI 1470 GTTAAC GTT^AAC
HpaII 38 CCGG C^CGG
MaeII 407 ACGT A^CGT
MamI 1066 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 668 TGGCCA TGG^CCA
MscI 668 TGGCCA TGG^CCA
MspI 38 CCGG C^CGG
MwoI 41 GCAGCGCCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 37 CCCGG CC^[GC]GG
NdeI 1141 CATATG CA^TATG
NspI 1509 GCATGT [GA]CATG^[CT]
PleI 76 GACTC GAGTC(4/5)
PpuMI 551 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 454 CAGCTG CAG^CTG
RcaI 476 TCATGA T^CATGA
SacI 292 GAGCTC GAGCT^C
SapI 438 GCTCTTC GCTCTTC(1/4)
Sau96I 552 GGACC G^G[ACGT]CC
SspBI 163 TGTACA T^GTACA
XmnI 732 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 603 GTATAC GT^[AC][GT]AC
1513 GTCTAC
AseI 850 ATTAAT AT^TAAT
1262 ATTAAT
AsnI 850 ATTAAT AT^TAAT
1262 ATTAAT
BsiYI 48 CCGCGCCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
109 CCTTCGCGCGG
BslI 48 CCGCGCCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
109 CCTTCGCGCGG
BsmFI 1370 GTCCC GGGAC(10/14)
2290 GTCCC
BspMI 182 ACCTGC ACCTGC(4/8)
554 ACCTGC
DraI 1193 TTTAAA TTT^AAA
2026 TTTAAA
EaeI 668 TGGCCA [CT]^GGCC[GA]
961 TGGCCG
EarI 439 CTCTTC CTCTTC(1/4)
421 GAAGAG
EcoRI 472 GAATTC G^AATTC
2323 GAATTC
EcoT14I 1813 CCAAGG C^C[AT][AT]GG
2254 CCTAGG
FauI 47 CCCGC CCCGC(4/6)
158 CCCGC
HaeII 33 AGCGCC [GA]GCGC^[CT]
43 AGCGCC
HincII 1470 GTTAAC GT[CT]^[GA]AC
1691 GTCAAC
HindII 1470 GTTAAC GT[CT]^[GA]AC
1691 GTCAAC
Ksp632I 439 CTCTTC CTCTTC(1/4)
421 GAAGAG
MspA1I 159 CCGCTG C[AC]G^C[GT]G
454 CAGCTG
NlaIV 227 GGCTCC GG[ACGT]^[ACGT]CC
1524 GGATCC
PshBI 850 ATTAAT AT^TAAT
1262 ATTAAT
PstI 184 CTGCAG CTGCA^G
274 CTGCAG
SfcI 184 CTGCAG C^T[GA][CT]AG
274 CTGCAG
SmlI 535 CTTAAG C^T[CT][GA]AG
2366 CTCAAG
StyI 1813 CCAAGG C^C[AT][AT]GG
2254 CCTAGG
TaqI 218 TCGA T^CGA
236 TCGA
Tsp45I 1306 GTCAC ^GT[GC]AC
1556 GTGAC
TthHB8I 218 TCGA T^CGA
236 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI BbvI
BfaI BlpI BmyI Bpu1102I BsaJI
BsiHKAI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MvaI MvnI NdeII NlaIII RsaI
Sau3AI ScrFI SfaNI SspI TfiI
Tru9I TseI Tsp509I TspRI XhoII