##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BalI BbeI BciVI BclI BfrI
BlnI BsaBI BsaI BsiEI BsiWI
Bsp1407I BspDI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MscI MseI
MunI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RsaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Aor51HI 101 AGCGCT AGC^GCT
Asp700I 1255 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 469 CCCGGG C^[CT]CG[GA]G
BbrPI 29 CACGTG CAC^GTG
BbsI 1316 GAAGAC GAAGAC(2/6)
BcgI 1275 GCACTGGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1197 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 622 AGATCT A^GATCT
BpuAI 1316 GAAGAC GAAGAC(2/6)
BsaAI 29 CACGTG [CT]AC^GT[GA]
BseRI 609 CTCCTC GAGGAG(10/8)
BsmBI 1188 CGTCTC CGTCTC(1/5)
BsmI 529 GAATGC GAATGC(1/-1)
BsoBI 469 CCCGGG C^[CT]CG[GA]G
BspHI 730 TCATGA T^CATGA
BsrDI 1127 CATTGC GCAATG(2/0)
BsrFI 1498 ACCGGC [GA]^CCGG[CT]
BstAPI 932 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1498 ACCGGC [GA]^CCGG[CT]
Eco47III 101 AGCGCT AGC^GCT
EcoNI 728 CCTCATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MslI 1172 CATGACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 1116 GCTAGC G^CTAGC
PflMI 962 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 29 CACGTG CAC^GTG
PmlI 29 CACGTG CAC^GTG
PshAI 1182 GACGCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 730 TCATGA T^CATGA
SapI 852 GAAGAGC GCTCTTC(1/4)
SmaI 469 CCCGGG CCC^GGG
StuI 514 AGGCCT AGG^CCT
Van91I 962 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 469 CCCGGG C^CCGGG
XmaI 469 CCCGGG C^CCGGG
XmnI 1255 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 227 TCCGGA T^CCGGA
376 TCCGGA
AcyI 1182 GACGCC G[GA]^CG[CT]C
1517 GGCGTC
AlwNI 330 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1543 CAGCAGCTG
ApaI 88 GGGCCC GGGCC^C
925 GGGCCC
AspHI 309 GAGCTC G[AT]GC[AT]^C
462 GAGCTC
BanI 956 GGTGCC G^G[CT][GA]CC
1095 GGCACC
BfaI 99 CTAG C^TAG
1117 CTAG
BlpI 59 GCTGAGC GC^T[ACGT]AGC
1013 GCTGAGC
Bpu1102I 59 GCTGAGC GC^T[ACGT]AGC
1013 GCTGAGC
BsaHI 1182 GACGCC G[GA]^CG[CT]C
1517 GGCGTC
BsaWI 227 TCCGGA [AT]^CCGG[AT]
376 TCCGGA
BseAI 227 TCCGGA T^CCGGA
376 TCCGGA
BsiHKAI 309 GAGCTC G[AT]GC[AT]^C
462 GAGCTC
BspEI 227 TCCGGA T^CCGGA
376 TCCGGA
CelII 59 GCTGAGC GC^T[ACGT]AGC
1013 GCTGAGC
DsaI 73 CCATGG C^C[GA][CT]GG
901 CCATGG
Hin1I 1182 GACGCC G[GA]^CG[CT]C
1517 GGCGTC
MaeI 99 CTAG C^TAG
1117 CTAG
MaeII 30 ACGT A^CGT
582 ACGT
MroI 227 TCCGGA T^CCGGA
376 TCCGGA
NcoI 73 CCATGG C^CATGG
901 CCATGG
NspI 119 ACATGC [GA]CATG^[CT]
973 ACATGC
PpuMI 1057 GGGTCCT [GA]G^G[AT]CC[CT]
1589 AGGACCC
SacI 309 GAGCTC GAGCT^C
462 GAGCTC
TaqI 313 TCGA T^CGA
892 TCGA
TfiI 1255 GAATC G^A[AT]TC
1435 GATTC
TthHB8I 313 TCGA T^CGA
892 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AluI AlwI AocI
AvaII AviII BamHI BanII BbvCI
BbvI BcnI BmyI BpmI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I TspRI XcmI XhoII