##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspHI AspI AvaII AviII AvrII
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrGI
BssSI Bst1107I BstAPI BstBI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI Eco47III EcoNI EcoT22I FbaI
FseI FspI Hin1I HindIII HpaI
KasI KpnI KspI MaeII MamI
MluI MroI NaeI NarI NdeI
NgoMIV NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfaNI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AhdI 434 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 897 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 237 CCTCAGG CC^T[ACGT]AGG
ApaI 422 GGGCCC GGGCC^C
AspEI 434 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1141 GGATCC G^GATCC
BbsI 1299 GTCTTC GAAGAC(2/6)
BpuAI 1299 GTCTTC GAAGAC(2/6)
BsaI 153 GGTCTC GGTCTC(1/5)
BsiEI 283 CGGCCG CG[GA][CT]^CG
BspHI 545 TCATGA T^CATGA
BsrDI 1014 CATTGC GCAATG(2/0)
BsrFI 150 GCCGGT [GA]^CCGG[CT]
BstEII 1353 GGTGACC G^GT[ACGT]ACC
BstPI 1353 GGTGACC G^GT[ACGT]ACC
BstXI 980 CCACACCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 237 CCTCAGG CC^T[ACGT]AGG
Cfr10I 150 GCCGGT [GA]^CCGG[CT]
DsaI 1160 CCATGG C^C[GA][CT]GG
EagI 283 CGGCCG C^GGCCG
Eam1105I 434 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 489 GAAGAG CTCTTC(1/4)
EclXI 283 CGGCCG C^GGCCG
Eco52I 283 CGGCCG C^GGCCG
Eco81I 237 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1353 GGTGACC G^GT[ACGT]ACC
EcoRV 541 GATATC GAT^ATC
HincII 1023 GTCAAC GT[CT]^[GA]AC
HindII 1023 GTCAAC GT[CT]^[GA]AC
Ksp632I 489 GAAGAG CTCTTC(1/4)
MfeI 1211 CAATTG C^AATTG
MunI 1211 CAATTG C^AATTG
NcoI 1160 CCATGG C^CATGG
NheI 751 GCTAGC G^CTAGC
NotI 282 GCGGCCGC GC^GGCCGC
PleI 370 GAGTC GAGTC(4/5)
PstI 1294 CTGCAG CTGCA^G
RcaI 545 TCATGA T^CATGA
ScaI 873 AGTACT AGT^ACT
TfiI 1553 GAATC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1327 GTCTAC GT^[AC][GT]AC
1715 GTAGAC
BalI 701 TGGCCA TGG^CCA
1157 TGGCCA
BssHII 380 GCGCGC G^CGCGC
395 GCGCGC
DraII 422 GGGCCCC [GA]G^G[ACGT]CC[CT]
558 AGGGCCT
EcoO109I 422 GGGCCCC [GA]G^G[ACGT]CC[CT]
558 AGGGCCT
EcoRI 866 GAATTC G^AATTC
1314 GAATTC
EcoT14I 1160 CCATGG C^C[AT][AT]GG
1415 CCAAGG
HinfI 370 GAGTC G^A[ACGT]TC
1553 GAATC
MluNI 701 TGGCCA TGG^CCA
1157 TGGCCA
MscI 701 TGGCCA TGG^CCA
1157 TGGCCA
MseI 1776 TTAA T^TAA
1857 TTAA
NspI 1635 ACATGC [GA]CATG^[CT]
1737 ACATGC
PaeR7I 222 CTCGAG C^TCGAG
304 CTCGAG
PvuII 822 CAGCTG CAG^CTG
1478 CAGCTG
SmaI 134 CCCGGG CCC^GGG
216 CCCGGG
StuI 454 AGGCCT AGG^CCT
1030 AGGCCT
StyI 1160 CCATGG C^C[AT][AT]GG
1415 CCAAGG
TaqI 223 TCGA T^CGA
305 TCGA
Tru9I 1776 TTAA T^TAA
1857 TTAA
TthHB8I 223 TCGA T^CGA
305 TCGA
XcmI 1145 CCATGCTTGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1160 CCATGGTTAGTCTGG
XhoI 222 CTCGAG C^TCGAG
304 CTCGAG
XmaCI 134 CCCGGG C^CCGGG
216 CCCGGG
XmaI 134 CCCGGG C^CCGGG
216 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AvaI BanII
BbvI BcnI BfaI BmyI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfcI SmlI
TseI Tsp45I Tsp509I TspRI XhoII
XmnI