##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
Hin1I HpaI KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MroI MunI MvnI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 1197 GTAGAC GT^[AC][GT]AC
AciI 1059 CCGC CCGC(-3/-1)
AflIII 101 ACATGT A^C[GA][CT]GT
AlwI 1126 GATCC GGATC(4/5)
Aor51HI 848 AGCGCT AGC^GCT
BalI 513 TGGCCA TGG^CCA
BbvCI 1048 GCTGAGG CCTCAGC(-5/-2)
BcgI 31 GCAGAAATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 1080 GTGCCC G[AGT]GC[ACT]^C
BpmI 844 CTGGAG CTGGAG(16/14)
BsaI 1000 GAGACC GGTCTC(1/5)
BsaWI 666 TCCGGT [AT]^CCGG[AT]
BseRI 700 GAGGAG GAGGAG(10/8)
BsgI 938 GTGCAG GTGCAG(16/14)
BsmFI 1092 GGGAC GGGAC(10/14)
BsmI 321 GAATGC GAATGC(1/-1)
Bsp1286I 1080 GTGCCC G[AGT]GC[ACT]^C
BspLU11I 101 ACATGT A^CATGT
BsrBI 1059 CCGCTC CCGCTC(-3/-3)
BstAPI 1167 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1125 AGATCC [GA]^GATC[CT]
DraII 1092 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1039 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 893 CCGTGG C^C[GA][CT]GG
Eco47III 848 AGCGCT AGC^GCT
EcoO109I 1092 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 326 CCAAGG C^C[AT][AT]GG
HaeII 848 AGCGCT [GA]GCGC^[CT]
HindIII 460 AAGCTT A^AGCTT
MflI 1125 AGATCC [GA]^GATC[CT]
MluNI 513 TGGCCA TGG^CCA
MscI 513 TGGCCA TGG^CCA
MslI 525 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 101 ACATGT [GA]CATG^[CT]
PflMI 1038 CCACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1092 GGGACCC [GA]G^G[AT]CC[CT]
SexAI 1192 ACCAGGT A^CC[AT]GGT
SfaNI 871 GATGC GCATC(5/9)
StyI 326 CCAAGG C^C[AT][AT]GG
Van91I 1038 CCACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1125 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 824 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1168 CAGCCACTG
AvaII 107 GGACC G^G[AT]CC
1093 GGACC
BcnI 132 CCGGG CC^[GC]GG
590 CCCGG
BsmAI 1000 GAGAC GTCTC(1/5)
1242 GAGAC
BsrFI 551 GCCGGC [GA]^CCGG[CT]
619 GCCGGC
BsrI 267 ACTGG ACTGG(1/-1)
389 ACTGG
BstXI 341 CCAAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
524 CCATGAACATGG
CfoI 849 GCGC GCG^C
1204 GCGC
Cfr10I 551 GCCGGC [GA]^CCGG[CT]
619 GCCGGC
EarI 469 CTCTTC CTCTTC(1/4)
1239 GAAGAG
EcoRI 585 GAATTC G^AATTC
628 GAATTC
HhaI 849 GCGC GCG^C
1204 GCGC
HinP1I 849 GCGC G^CGC
1204 GCGC
HincII 356 GTCAAC GT[CT]^[GA]AC
502 GTCAAC
HindII 356 GTCAAC GT[CT]^[GA]AC
502 GTCAAC
HphI 145 GGTGA GGTGA(8/7)
480 GGTGA
Ksp632I 469 CTCTTC CTCTTC(1/4)
1239 GAAGAG
MspA1I 273 CAGCTG C[AC]G^C[GT]G
824 CAGCTG
NaeI 551 GCCGGC GCC^GGC
619 GCCGGC
NciI 132 CCGGG CC^[GC]GG
590 CCCGG
NgoMIV 551 GCCGGC G^CCGGC
619 GCCGGC
PleI 1143 GACTC GAGTC(4/5)
354 GAGTC
PstI 879 CTGCAG CTGCA^G
905 CTGCAG
PvuII 273 CAGCTG CAG^CTG
824 CAGCTG
SfcI 879 CTGCAG C^T[GA][CT]AG
905 CTGCAG
Tsp45I 252 GTCAC ^GT[GC]AC
1029 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AcsI AluI ApoI BbvI BsaJI
BsiYI BslI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EaeI
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I