##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AlwNI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BbrPI
BcgI BciVI BfrI BglII BlnI
BsaBI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI Eam1105I EcoO65I EcoRI EcoRV
EcoT22I FseI HincII HindII HpaI
KpnI MamI MfeI MluI MroI
MseI MslI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
ScaI SexAI SfiI SfuI SmlI
SnaBI SpeI SspBI SspI SwaI
Tru9I Tth111I Van91I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AocI 1626 CCTGAGG CC^T[ACGT]AGG
BbvCI 1076 GCTGAGG CCTCAGC(-5/-2)
BclI 1839 TGATCA T^GATCA
BsaAI 629 TACGTG [CT]AC^GT[GA]
BsiEI 71 CGGCCG CG[GA][CT]^CG
BsmBI 484 CGTCTC CGTCTC(1/5)
BspMI 987 ACCTGC ACCTGC(4/8)
BssSI 1415 CTCGTG CACGAG(-5/-1)
BstXI 435 CCATCGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 295 GGATCT [GA]^GATC[CT]
Bsu36I 1626 CCTGAGG CC^T[ACGT]AGG
DraIII 1381 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 71 CGGCCG C^GGCCG
EclXI 71 CGGCCG C^GGCCG
Eco52I 71 CGGCCG C^GGCCG
Eco81I 1626 CCTGAGG CC^T[ACGT]AGG
FbaI 1839 TGATCA T^GATCA
HindIII 1801 AAGCTT A^AGCTT
MflI 295 GGATCT [GA]^GATC[CT]
PpuMI 1741 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1561 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 634 GCTCTTC GCTCTTC(1/4)
SbfI 907 CCTGCAGG CCTGCA^GG
SgrAI 78 CGCCGGTG C[GA]^CCGG[CT]G
SphI 1612 GCATGC GCATG^C
Sse8387I 907 CCTGCAGG CCTGCA^GG
StuI 624 AGGCCT AGG^CCT
XbaI 1957 TCTAGA T^CTAGA
XhoII 295 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 707 GTAC GT^AC
902 GTAC
Alw44I 678 GTGCAC G^TGCAC
1200 GTGCAC
AlwI 917 GATCC GGATC(4/5)
295 GGATC
Aor51HI 559 AGCGCT AGC^GCT
1143 AGCGCT
ApaI 473 GGGCCC GGGCC^C
844 GGGCCC
ApaLI 678 GTGCAC G^TGCAC
1200 GTGCAC
AvaII 753 GGACC G^G[AT]CC
1742 GGACC
AviII 801 TGCGCA TGC^GCA
1615 TGCGCA
BglI 730 GCCGGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
827 GCCTTTGTGGC
BsaI 1041 GAGACC GGTCTC(1/5)
1559 GAGACC
BseRI 542 CTCCTC GAGGAG(10/8)
1708 GAGGAG
BsgI 1899 CTGCAC GTGCAG(16/14)
1333 GTGCAG
BsrBI 344 CCGCTC CCGCTC(-3/-3)
611 CCGCTC
BstAPI 404 GCACTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
693 GCATCTACTGC
Eco47III 559 AGCGCT AGC^GCT
1143 AGCGCT
EcoNI 1679 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1828 CCTAATTCAGG
FspI 801 TGCGCA TGC^GCA
1615 TGCGCA
KspI 127 CCGCGG CCGC^GG
519 CCGCGG
MaeIII 1485 GTGAC ^GT[ACGT]AC
1540 GTGAC
NspI 836 GCATGT [GA]CATG^[CT]
1612 GCATGC
RsaI 707 GTAC GT^AC
902 GTAC
SacII 127 CCGCGG CCGC^GG
519 CCGCGG
TaqI 761 TCGA T^CGA
870 TCGA
Tsp45I 1485 GTGAC ^GT[GC]AC
1540 GTGAC
TthHB8I 761 TCGA T^CGA
870 TCGA
XcmI 557 CCAGCGCTGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2079 CCAAATCCTATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
ApoI AspHI AvaI BalI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MboI
MboII MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PleI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI StyI TfiI TseI Tsp509I
TspRI XmaCI XmaI