################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvrII BbeI BbrPI BbvCI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FauI FseI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 73 GACGTC GACGT^C AccII 59 CGCG CG^CG AflIII 956 ACGTGT A^C[GA][CT]GT AocI 184 CCTGAGG CC^T[ACGT]AGG AseI 1285 ATTAAT AT^TAAT AsnI 1285 ATTAAT AT^TAAT Asp700I 775 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 14 GAGCTC G[AT]GC[AT]^C AvaI 357 CCCGGG C^[CT]CG[GA]G AviII 1131 TGCGCA TGC^GCA BalI 313 TGGCCA TGG^CCA BamHI 801 GGATCC G^GATCC BanI 1301 GGTGCC G^G[CT][GA]CC BanII 14 GAGCTC G[GA]GC[CT]^C BcgI 855 CGAGTGGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 192 TGATCA T^GATCA BmyI 14 GAGCTC G[AGT]GC[ACT]^C BsiHKAI 14 GAGCTC G[AT]GC[AT]^C BsmAI 233 GAGAC GTCTC(1/5) BsmI 1134 GCATTC GAATGC(1/-1) BsoBI 357 CCCGGG C^[CT]CG[GA]G Bsp1286I 14 GAGCTC G[AGT]GC[ACT]^C BspHI 1080 TCATGA T^CATGA BspMI 1350 GCAGGT ACCTGC(4/8) BstEII 1231 GGTTACC G^GT[ACGT]ACC BstPI 1231 GGTTACC G^GT[ACGT]ACC BstUI 59 CGCG CG^CG BstXI 299 CCAGGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 184 CCTGAGG CC^T[ACGT]AGG DraII 353 AGGCCCC [GA]G^G[ACGT]CC[CT] Eco81I 184 CCTGAGG CC^T[ACGT]AGG EcoO109I 353 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoO65I 1231 GGTTACC G^GT[ACGT]ACC EcoT14I 1390 CCTTGG C^C[AT][AT]GG FbaI 192 TGATCA T^GATCA FspI 1131 TGCGCA TGC^GCA HaeII 1313 AGCGCC [GA]GCGC^[CT] HapII 358 CCGG C^CGG HpaII 358 CCGG C^CGG HphI 687 GGTGA GGTGA(8/7) MluNI 313 TGGCCA TGG^CCA MscI 313 TGGCCA TGG^CCA MspI 358 CCGG C^CGG MvnI 59 CGCG CG^CG PshBI 1285 ATTAAT AT^TAAT PvuII 1264 CAGCTG CAG^CTG RcaI 1080 TCATGA T^CATGA SacI 14 GAGCTC GAGCT^C SfcI 556 CTACAG C^T[GA][CT]AG SmaI 357 CCCGGG CCC^GGG StuI 949 AGGCCT AGG^CCT StyI 1390 CCTTGG C^C[AT][AT]GG XmaCI 357 CCCGGG C^CCGGG XmaI 357 CCCGGG C^CCGGG XmnI 775 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 718 GTAC GT^AC 997 GTAC AvaII 579 GGACC G^G[AT]CC 1394 GGACC BbsI 643 GAAGAC GAAGAC(2/6) 900 GAAGAC BcnI 357 CCCGG CC^[GC]GG 358 CCGGG BpmI 307 CTGGAG CTGGAG(16/14) 550 CTGGAG BpuAI 643 GAAGAC GAAGAC(2/6) 900 GAAGAC BsmFI 71 GGGAC GGGAC(10/14) 1126 GGGAC BsrDI 248 GCAATG GCAATG(2/0) 832 GCAATG BstYI 405 AGATCC [GA]^GATC[CT] 801 GGATCC EaeI 313 TGGCCA [CT]^GGCC[GA] 734 TGGCCG EarI 978 CTCTTC CTCTTC(1/4) 1329 CTCTTC HgaI 227 GCGTC GACGC(5/10) 563 GCGTC HincII 893 GTTAAC GT[CT]^[GA]AC 1409 GTTAAC HindII 893 GTTAAC GT[CT]^[GA]AC 1409 GTTAAC HindIII 241 AAGCTT A^AGCTT 884 AAGCTT HpaI 893 GTTAAC GTT^AAC 1409 GTTAAC Ksp632I 978 CTCTTC CTCTTC(1/4) 1329 CTCTTC MflI 405 AGATCC [GA]^GATC[CT] 801 GGATCC MslI 1194 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1282 CACATTAATG MspA1I 1188 CAGCGG C[AC]G^C[GT]G 1264 CAGCTG NciI 357 CCCGG CC^[GC]GG 358 CCGGG RsaI 718 GTAC GT^AC 997 GTAC SapI 977 GCTCTTC GCTCTTC(1/4) 1328 GCTCTTC SfaNI 829 GATGC GCATC(5/9) 908 GATGC TaqI 783 TCGA T^CGA 1689 TCGA TthHB8I 783 TCGA T^CGA 1689 TCGA XhoII 405 AGATCC [GA]^GATC[CT] 801 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AcsI AcyI AluI AlwI AlwNI ApoI BbvI BfaI BsaHI BsaJI BsgI BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraI EcoRII Fnu4HI FokI HaeIII HhaI Hin1I HinP1I HinfI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI TfiI Tru9I TseI Tsp45I Tsp509I TspRI