##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvrII BbeI BbrPI BbvCI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I CelII Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FauI
FseI KasI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 73 GACGTC GACGT^C
AccII 59 CGCG CG^CG
AflIII 956 ACGTGT A^C[GA][CT]GT
AocI 184 CCTGAGG CC^T[ACGT]AGG
AseI 1285 ATTAAT AT^TAAT
AsnI 1285 ATTAAT AT^TAAT
Asp700I 775 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 14 GAGCTC G[AT]GC[AT]^C
AvaI 357 CCCGGG C^[CT]CG[GA]G
AviII 1131 TGCGCA TGC^GCA
BalI 313 TGGCCA TGG^CCA
BamHI 801 GGATCC G^GATCC
BanI 1301 GGTGCC G^G[CT][GA]CC
BanII 14 GAGCTC G[GA]GC[CT]^C
BcgI 855 CGAGTGGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 192 TGATCA T^GATCA
BmyI 14 GAGCTC G[AGT]GC[ACT]^C
BsiHKAI 14 GAGCTC G[AT]GC[AT]^C
BsmAI 233 GAGAC GTCTC(1/5)
BsmI 1134 GCATTC GAATGC(1/-1)
BsoBI 357 CCCGGG C^[CT]CG[GA]G
Bsp1286I 14 GAGCTC G[AGT]GC[ACT]^C
BspHI 1080 TCATGA T^CATGA
BspMI 1350 GCAGGT ACCTGC(4/8)
BstEII 1231 GGTTACC G^GT[ACGT]ACC
BstPI 1231 GGTTACC G^GT[ACGT]ACC
BstUI 59 CGCG CG^CG
BstXI 299 CCAGGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 184 CCTGAGG CC^T[ACGT]AGG
DraII 353 AGGCCCC [GA]G^G[ACGT]CC[CT]
Eco81I 184 CCTGAGG CC^T[ACGT]AGG
EcoO109I 353 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1231 GGTTACC G^GT[ACGT]ACC
EcoT14I 1390 CCTTGG C^C[AT][AT]GG
FbaI 192 TGATCA T^GATCA
FspI 1131 TGCGCA TGC^GCA
HaeII 1313 AGCGCC [GA]GCGC^[CT]
HapII 358 CCGG C^CGG
HpaII 358 CCGG C^CGG
HphI 687 GGTGA GGTGA(8/7)
MluNI 313 TGGCCA TGG^CCA
MscI 313 TGGCCA TGG^CCA
MspI 358 CCGG C^CGG
MvnI 59 CGCG CG^CG
PshBI 1285 ATTAAT AT^TAAT
PvuII 1264 CAGCTG CAG^CTG
RcaI 1080 TCATGA T^CATGA
SacI 14 GAGCTC GAGCT^C
SfcI 556 CTACAG C^T[GA][CT]AG
SmaI 357 CCCGGG CCC^GGG
StuI 949 AGGCCT AGG^CCT
StyI 1390 CCTTGG C^C[AT][AT]GG
XmaCI 357 CCCGGG C^CCGGG
XmaI 357 CCCGGG C^CCGGG
XmnI 775 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 718 GTAC GT^AC
997 GTAC
AvaII 579 GGACC G^G[AT]CC
1394 GGACC
BbsI 643 GAAGAC GAAGAC(2/6)
900 GAAGAC
BcnI 357 CCCGG CC^[GC]GG
358 CCGGG
BpmI 307 CTGGAG CTGGAG(16/14)
550 CTGGAG
BpuAI 643 GAAGAC GAAGAC(2/6)
900 GAAGAC
BsmFI 71 GGGAC GGGAC(10/14)
1126 GGGAC
BsrDI 248 GCAATG GCAATG(2/0)
832 GCAATG
BstYI 405 AGATCC [GA]^GATC[CT]
801 GGATCC
EaeI 313 TGGCCA [CT]^GGCC[GA]
734 TGGCCG
EarI 978 CTCTTC CTCTTC(1/4)
1329 CTCTTC
HgaI 227 GCGTC GACGC(5/10)
563 GCGTC
HincII 893 GTTAAC GT[CT]^[GA]AC
1409 GTTAAC
HindII 893 GTTAAC GT[CT]^[GA]AC
1409 GTTAAC
HindIII 241 AAGCTT A^AGCTT
884 AAGCTT
HpaI 893 GTTAAC GTT^AAC
1409 GTTAAC
Ksp632I 978 CTCTTC CTCTTC(1/4)
1329 CTCTTC
MflI 405 AGATCC [GA]^GATC[CT]
801 GGATCC
MslI 1194 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1282 CACATTAATG
MspA1I 1188 CAGCGG C[AC]G^C[GT]G
1264 CAGCTG
NciI 357 CCCGG CC^[GC]GG
358 CCGGG
RsaI 718 GTAC GT^AC
997 GTAC
SapI 977 GCTCTTC GCTCTTC(1/4)
1328 GCTCTTC
SfaNI 829 GATGC GCATC(5/9)
908 GATGC
TaqI 783 TCGA T^CGA
1689 TCGA
TthHB8I 783 TCGA T^CGA
1689 TCGA
XhoII 405 AGATCC [GA]^GATC[CT]
801 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AcyI AluI AlwI
AlwNI ApoI BbvI BfaI BsaHI
BsaJI BsgI BsiYI BslI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraI EcoRII
Fnu4HI FokI HaeIII HhaI Hin1I
HinP1I HinfI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI