################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AccII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I CelII ClaI CpoI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoRI EcoT22I FbaI FseI FspI HaeII HincII HindII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 246 GTCTAC GT^[AC][GT]AC AccIII 283 TCCGGA T^CCGGA AcsI 1077 AAATTT [GA]^AATT[CT] AlwNI 452 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 1077 AAATTT [GA]^AATT[CT] AspHI 138 GAGCTC G[AT]GC[AT]^C AspI 1241 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 863 GGCACC G^G[CT][GA]CC BbvCI 1525 GCTGAGG CCTCAGC(-5/-2) BcgI 1336 CGAAGGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 508 CCCGG CC^[GC]GG BsaWI 283 TCCGGA [AT]^CCGG[AT] BseAI 283 TCCGGA T^CCGGA BsiHKAI 138 GAGCTC G[AT]GC[AT]^C BspEI 283 TCCGGA T^CCGGA BspHI 377 TCATGA T^CATGA BspMI 866 ACCTGC ACCTGC(4/8) BsrBI 1307 CCGCTC CCGCTC(-3/-3) BstAPI 1685 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 596 GGTCACC G^GT[ACGT]ACC BstPI 596 GGTCACC G^GT[ACGT]ACC BstYI 548 GGATCT [GA]^GATC[CT] CfoI 1224 GCGC GCG^C DraI 120 TTTAAA TTT^AAA EcoNI 214 CCTACTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 596 GGTCACC G^GT[ACGT]ACC EcoRV 581 GATATC GAT^ATC HhaI 1224 GCGC GCG^C HinP1I 1224 GCGC G^CGC HindIII 588 AAGCTT A^AGCTT MflI 548 GGATCT [GA]^GATC[CT] MroI 283 TCCGGA T^CCGGA MslI 641 CACAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 508 CCCGG CC^[GC]GG NcoI 1312 CCATGG C^CATGG PaeR7I 1417 CTCGAG C^TCGAG PflFI 1241 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1317 GGGACCC [GA]G^G[AT]CC[CT] PshAI 50 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 377 TCATGA T^CATGA SacI 138 GAGCTC GAGCT^C SexAI 720 ACCTGGT A^CC[AT]GGT SmlI 1417 CTCGAG C^T[CT][GA]AG TfiI 293 GATTC G^A[AT]TC Tth111I 1241 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 620 TCTAGA T^CTAGA XhoI 1417 CTCGAG C^TCGAG XhoII 548 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 78 GACGCC G[GA]^CG[CT]C 680 GGCGTC AfaI 690 GTAC GT^AC 725 GTAC AlwI 548 GGATC GGATC(4/5) 1666 GGATC AocI 1432 CCTCAGG CC^T[ACGT]AGG 1543 CCTGAGG Asp700I 189 GAACGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 266 GAAGTCCTTC AvaI 35 CTCGGG C^[CT]CG[GA]G 1417 CTCGAG BglI 848 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1572 GCCAACATGGC BsaHI 78 GACGCC G[GA]^CG[CT]C 680 GGCGTC BsoBI 35 CTCGGG C^[CT]CG[GA]G 1417 CTCGAG BsrDI 1028 CATTGC GCAATG(2/0) 228 GCAATG Bsu36I 1432 CCTCAGG CC^T[ACGT]AGG 1543 CCTGAGG DraII 315 AGGCCCT [GA]G^G[ACGT]CC[CT] 1317 GGGACCC DsaI 1125 CCGTGG C^C[GA][CT]GG 1312 CCATGG Eco81I 1432 CCTCAGG CC^T[ACGT]AGG 1543 CCTGAGG EcoO109I 315 AGGCCCT [GA]G^G[ACGT]CC[CT] 1317 GGGACCC EcoT14I 388 CCAAGG C^C[AT][AT]GG 1312 CCATGG FauI 1177 GCGGG CCCGC(4/6) 1531 GCGGG HgaI 78 GACGC GACGC(5/10) 681 GCGTC Hin1I 78 GACGCC G[GA]^CG[CT]C 680 GGCGTC MseI 121 TTAA T^TAA 157 TTAA NaeI 472 GCCGGC GCC^GGC 860 GCCGGC NgoMIV 472 GCCGGC G^CCGGC 860 GCCGGC PflMI 486 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1188 CCACCTTCTGG PvuII 1103 CAGCTG CAG^CTG 1403 CAGCTG RsaI 690 GTAC GT^AC 725 GTAC StyI 388 CCAAGG C^C[AT][AT]GG 1312 CCATGG Tru9I 121 TTAA T^TAA 157 TTAA Van91I 486 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1188 CCACCTTCTGG XcmI 843 CCAAAGCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1243 CCAAGTCTGAGCTGG XmnI 189 GAACGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 266 GAAGTCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AluI AvaII BalI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr10I Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PstI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I