##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbeI BbrPI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
CelII ClaI CpoI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoRI EcoT22I FbaI FseI
FspI HaeII HincII HindII HpaI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MunI MvnI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 246 GTCTAC GT^[AC][GT]AC
AccIII 283 TCCGGA T^CCGGA
AcsI 1077 AAATTT [GA]^AATT[CT]
AlwNI 452 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1077 AAATTT [GA]^AATT[CT]
AspHI 138 GAGCTC G[AT]GC[AT]^C
AspI 1241 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 863 GGCACC G^G[CT][GA]CC
BbvCI 1525 GCTGAGG CCTCAGC(-5/-2)
BcgI 1336 CGAAGGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 508 CCCGG CC^[GC]GG
BsaWI 283 TCCGGA [AT]^CCGG[AT]
BseAI 283 TCCGGA T^CCGGA
BsiHKAI 138 GAGCTC G[AT]GC[AT]^C
BspEI 283 TCCGGA T^CCGGA
BspHI 377 TCATGA T^CATGA
BspMI 866 ACCTGC ACCTGC(4/8)
BsrBI 1307 CCGCTC CCGCTC(-3/-3)
BstAPI 1685 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 596 GGTCACC G^GT[ACGT]ACC
BstPI 596 GGTCACC G^GT[ACGT]ACC
BstYI 548 GGATCT [GA]^GATC[CT]
CfoI 1224 GCGC GCG^C
DraI 120 TTTAAA TTT^AAA
EcoNI 214 CCTACTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 596 GGTCACC G^GT[ACGT]ACC
EcoRV 581 GATATC GAT^ATC
HhaI 1224 GCGC GCG^C
HinP1I 1224 GCGC G^CGC
HindIII 588 AAGCTT A^AGCTT
MflI 548 GGATCT [GA]^GATC[CT]
MroI 283 TCCGGA T^CCGGA
MslI 641 CACAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 508 CCCGG CC^[GC]GG
NcoI 1312 CCATGG C^CATGG
PaeR7I 1417 CTCGAG C^TCGAG
PflFI 1241 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1317 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 50 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 377 TCATGA T^CATGA
SacI 138 GAGCTC GAGCT^C
SexAI 720 ACCTGGT A^CC[AT]GGT
SmlI 1417 CTCGAG C^T[CT][GA]AG
TfiI 293 GATTC G^A[AT]TC
Tth111I 1241 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 620 TCTAGA T^CTAGA
XhoI 1417 CTCGAG C^TCGAG
XhoII 548 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 78 GACGCC G[GA]^CG[CT]C
680 GGCGTC
AfaI 690 GTAC GT^AC
725 GTAC
AlwI 548 GGATC GGATC(4/5)
1666 GGATC
AocI 1432 CCTCAGG CC^T[ACGT]AGG
1543 CCTGAGG
Asp700I 189 GAACGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
266 GAAGTCCTTC
AvaI 35 CTCGGG C^[CT]CG[GA]G
1417 CTCGAG
BglI 848 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1572 GCCAACATGGC
BsaHI 78 GACGCC G[GA]^CG[CT]C
680 GGCGTC
BsoBI 35 CTCGGG C^[CT]CG[GA]G
1417 CTCGAG
BsrDI 1028 CATTGC GCAATG(2/0)
228 GCAATG
Bsu36I 1432 CCTCAGG CC^T[ACGT]AGG
1543 CCTGAGG
DraII 315 AGGCCCT [GA]G^G[ACGT]CC[CT]
1317 GGGACCC
DsaI 1125 CCGTGG C^C[GA][CT]GG
1312 CCATGG
Eco81I 1432 CCTCAGG CC^T[ACGT]AGG
1543 CCTGAGG
EcoO109I 315 AGGCCCT [GA]G^G[ACGT]CC[CT]
1317 GGGACCC
EcoT14I 388 CCAAGG C^C[AT][AT]GG
1312 CCATGG
FauI 1177 GCGGG CCCGC(4/6)
1531 GCGGG
HgaI 78 GACGC GACGC(5/10)
681 GCGTC
Hin1I 78 GACGCC G[GA]^CG[CT]C
680 GGCGTC
MseI 121 TTAA T^TAA
157 TTAA
NaeI 472 GCCGGC GCC^GGC
860 GCCGGC
NgoMIV 472 GCCGGC G^CCGGC
860 GCCGGC
PflMI 486 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1188 CCACCTTCTGG
PvuII 1103 CAGCTG CAG^CTG
1403 CAGCTG
RsaI 690 GTAC GT^AC
725 GTAC
StyI 388 CCAAGG C^C[AT][AT]GG
1312 CCATGG
Tru9I 121 TTAA T^TAA
157 TTAA
Van91I 486 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1188 CCACCTTCTGG
XcmI 843 CCAAAGCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1243 CCAAGTCTGAGCTGG
XmnI 189 GAACGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
266 GAAGTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AluI AvaII BalI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstXI Cac8I Cfr10I Cfr13I DdeI
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I