##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AhdI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BamHI BbeI BbrPI
BciVI BclI BfaI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstXI
BstZ17I CelII ClaI CpoI DraI
DrdI Eam1105I EcoNI EcoRI EcoRV
EcoT22I FbaI FspI HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MluNI MscI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI XbaI
XcmI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 118 GTCGAC GT^[AC][GT]AC
AccIII 785 TCCGGA T^CCGGA
AgeI 1421 ACCGGT A^CCGGT
Alw44I 217 GTGCAC G^TGCAC
Aor51HI 410 AGCGCT AGC^GCT
ApaI 1459 GGGCCC GGGCC^C
ApaLI 217 GTGCAC G^TGCAC
AspHI 217 GTGCAC G[AT]GC[AT]^C
AspI 769 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1529 GGCACC G^G[CT][GA]CC
BbsI 1903 GAAGAC GAAGAC(2/6)
BglI 1437 GCCCGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1903 GAAGAC GAAGAC(2/6)
BsaI 507 GAGACC GGTCTC(1/5)
BseAI 785 TCCGGA T^CCGGA
BsgI 427 GTGCAG GTGCAG(16/14)
BsiHKAI 217 GTGCAC G[AT]GC[AT]^C
BspEI 785 TCCGGA T^CCGGA
BssHII 4 GCGCGC G^CGCGC
BssSI 76 CACGAG CACGAG(-5/-1)
BstAPI 1090 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 974 GGTCACC G^GT[ACGT]ACC
BstPI 974 GGTCACC G^GT[ACGT]ACC
DraIII 66 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 410 AGCGCT AGC^GCT
EcoO65I 974 GGTCACC G^GT[ACGT]ACC
FseI 1697 GGCCGGCC GGCCGG^CC
HincII 118 GTCGAC GT[CT]^[GA]AC
HindII 118 GTCGAC GT[CT]^[GA]AC
HindIII 100 AAGCTT A^AGCTT
MroI 785 TCCGGA T^CCGGA
MseI 260 TTAA T^TAA
NcoI 1919 CCATGG C^CATGG
NspI 819 GCATGC [GA]CATG^[CT]
PaeR7I 1227 CTCGAG C^TCGAG
PflFI 769 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 65 CCACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1421 ACCGGT A^CCGGT
PvuII 1557 CAGCTG CAG^CTG
SalI 118 GTCGAC G^TCGAC
SapI 2039 GCTCTTC GCTCTTC(1/4)
SbfI 189 CCTGCAGG CCTGCA^GG
SphI 819 GCATGC GCATG^C
Sse8387I 189 CCTGCAGG CCTGCA^GG
Tru9I 260 TTAA T^TAA
Tsp509I 704 AATT ^AATT
TspRI 460 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 769 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 65 CCACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1227 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AocI 1524 CCTCAGG CC^T[ACGT]AGG
1789 CCTCAGG
BbvCI 1566 CCTCAGC CCTCAGC(-5/-2)
903 GCTGAGG
BglII 473 AGATCT A^GATCT
726 AGATCT
BsaWI 785 TCCGGA [AT]^CCGG[AT]
1421 ACCGGT
BsmBI 144 GAGACG CGTCTC(1/5)
1412 GAGACG
Bsu36I 1524 CCTCAGG CC^T[ACGT]AGG
1789 CCTCAGG
Eco81I 1524 CCTCAGG CC^T[ACGT]AGG
1789 CCTCAGG
EcoT14I 824 CCAAGG C^C[AT][AT]GG
1919 CCATGG
PstI 190 CTGCAG CTGCA^G
230 CTGCAG
SmaI 1026 CCCGGG CCC^GGG
1167 CCCGGG
StuI 420 AGGCCT AGG^CCT
498 AGGCCT
StyI 824 CCAAGG C^C[AT][AT]GG
1919 CCATGG
TfiI 226 GATTC G^A[AT]TC
305 GATTC
XmaCI 1026 CCCGGG C^CCGGG
1167 CCCGGG
XmaI 1026 CCCGGG C^CCGGG
1167 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BanII
BbvI BcgI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsiEI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TseI Tsp45I TthHB8I
XhoII