##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspI AvrII BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BsaBI BsiEI BspDI BspHI
BspMI BsrBI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspI StuI SwaI TaqI
Tth111I TthHB8I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 544 TCCGGA T^CCGGA
AflIII 1131 ACATGT A^C[GA][CT]GT
AhdI 4 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1641 GGGCCC GGGCC^C
Asp700I 1375 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1782 GAGCTC G[AT]GC[AT]^C
AviII 1026 TGCGCA TGC^GCA
BalI 174 TGGCCA TGG^CCA
BbsI 751 GTCTTC GAAGAC(2/6)
BlpI 1204 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1204 GCTTAGC GC^T[ACGT]AGC
BpuAI 751 GTCTTC GAAGAC(2/6)
BsaAI 1085 TACGTG [CT]AC^GT[GA]
BsaI 1649 GGTCTC GGTCTC(1/5)
BsaWI 544 TCCGGA [AT]^CCGG[AT]
BseAI 544 TCCGGA T^CCGGA
BsiHKAI 1782 GAGCTC G[AT]GC[AT]^C
BsiWI 1083 CGTACG C^GTACG
BsmBI 1166 CGTCTC CGTCTC(1/5)
Bsp1407I 1626 TGTACA T^GTACA
BspEI 544 TCCGGA T^CCGGA
BspLU11I 1131 ACATGT A^CATGT
BsrDI 666 GCAATG GCAATG(2/0)
BsrGI 1626 TGTACA T^GTACA
BstXI 361 CCAGCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1204 GCTTAGC GC^T[ACGT]AGC
DrdI 488 GACACTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1560 CTCTTC CTCTTC(1/4)
EcoT14I 724 CCATGG C^C[AT][AT]GG
FspI 1026 TGCGCA TGC^GCA
Ksp632I 1560 CTCTTC CTCTTC(1/4)
MaeII 1086 ACGT A^CGT
MluNI 174 TGGCCA TGG^CCA
MroI 544 TCCGGA T^CCGGA
MscI 174 TGGCCA TGG^CCA
NcoI 724 CCATGG C^CATGG
NspI 1131 ACATGT [GA]CATG^[CT]
PpuMI 304 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 1782 GAGCTC GAGCT^C
SbfI 1579 CCTGCAGG CCTGCA^GG
ScaI 198 AGTACT AGT^ACT
SmlI 980 CTCAAG C^T[CT][GA]AG
Sse8387I 1579 CCTGCAGG CCTGCA^GG
SspBI 1626 TGTACA T^GTACA
StyI 724 CCATGG C^C[AT][AT]GG
XcmI 1655 CCATAGACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1375 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 707 GTCTAC GT^[AC][GT]AC
1247 GTAGAC
AccII 41 CGCG CG^CG
67 CGCG
BamHI 265 GGATCC G^GATCC
631 GGATCC
BbeI 19 GGCGCC GGCGC^C
1412 GGCGCC
BseRI 332 CTCCTC GAGGAG(10/8)
676 CTCCTC
BsgI 1042 CTGCAC GTGCAG(16/14)
1313 GTGCAG
BsmAI 1167 GTCTC GTCTC(1/5)
1650 GTCTC
BsmI 815 GAATGC GAATGC(1/-1)
1738 GAATGC
BstUI 41 CGCG CG^CG
67 CGCG
DsaI 724 CCATGG C^C[GA][CT]GG
1514 CCACGG
HgaI 39 GACGC GACGC(5/10)
119 GCGTC
HphI 375 TCACC GGTGA(8/7)
1455 TCACC
KasI 19 GGCGCC G^GCGCC
1412 GGCGCC
MseI 1733 TTAA T^TAA
1844 TTAA
MslI 934 CACTGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1488 CATACTGATG
MvnI 41 CGCG CG^CG
67 CGCG
NarI 19 GGCGCC GG^CGCC
1412 GGCGCC
PflMI 946 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1300 CCAGAAGATGG
PstI 313 CTGCAG CTGCA^G
1580 CTGCAG
SfcI 313 CTGCAG C^T[GA][CT]AG
1580 CTGCAG
SfoI 19 GGCGCC GGC^GCC
1412 GGCGCC
SmaI 15 CCCGGG CCC^GGG
167 CCCGGG
Tru9I 1733 TTAA T^TAA
1844 TTAA
Tsp45I 374 GTCAC ^GT[GC]AC
821 GTGAC
Van91I 946 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1300 CCAGAAGATGG
XmaCI 15 CCCGGG C^CCGGG
167 CCCGGG
XmaI 15 CCCGGG C^CCGGG
167 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AvaI AvaII BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaJI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII ItaI
MaeI MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI TseI
Tsp509I TspRI XhoII