##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI AlwI Aor51HI
AscI AseI AsnI Asp700I AspEI
AvrII BamHI BbsI BcgI BclI
BfrI BlnI BpuAI BsaBI BseAI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FbaI FseI HindIII HpaI KspI
MamI MfeI MluI MroI MseI
MunI NdeI NotI NruI NsiI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
ApaI 1508 GGGCCC GGGCC^C
AspI 740 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 882 CACGTG CAC^GTG
BbvCI 1704 CCTCAGC CCTCAGC(-5/-2)
BciVI 1416 GGATAC GTATCC(6/5)
BglI 204 GCCGGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 934 AGATCT A^GATCT
BsaAI 882 CACGTG [CT]AC^GT[GA]
BsaI 1652 GAGACC GGTCTC(1/5)
BsaWI 775 ACCGGA [AT]^CCGG[AT]
BsmBI 1306 GAGACG CGTCTC(1/5)
BspHI 763 TCATGA T^CATGA
BsrBI 1468 GAGCGG CCGCTC(-3/-3)
BsrI 688 ACTGG ACTGG(1/-1)
BssSI 300 CTCGTG CACGAG(-5/-1)
BstYI 934 AGATCT [GA]^GATC[CT]
DrdI 323 GACCACGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1685 CTCTTC CTCTTC(1/4)
EcoRI 1487 GAATTC G^AATTC
FauI 1238 GCGGG CCCGC(4/6)
HgaI 330 GCGTC GACGC(5/10)
HincII 663 GTCAAC GT[CT]^[GA]AC
HindII 663 GTCAAC GT[CT]^[GA]AC
HinfI 740 GACTC G^A[ACGT]TC
Ksp632I 1685 CTCTTC CTCTTC(1/4)
MflI 934 AGATCT [GA]^GATC[CT]
NheI 9 GCTAGC G^CTAGC
NspI 1078 GCATGT [GA]CATG^[CT]
PaeR7I 894 CTCGAG C^TCGAG
PflFI 740 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 740 GACTC GAGTC(4/5)
PmaCI 882 CACGTG CAC^GTG
PmlI 882 CACGTG CAC^GTG
RcaI 763 TCATGA T^CATGA
SmlI 894 CTCGAG C^T[CT][GA]AG
StuI 782 AGGCCT AGG^CCT
TaqI 895 TCGA T^CGA
Tth111I 740 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 895 TCGA T^CGA
XhoI 894 CTCGAG C^TCGAG
XhoII 934 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 1404 GGTACC G^GTACC
1635 GGTACC
AcsI 699 AAATTC [GA]^AATT[CT]
1487 GAATTC
AlwNI 145 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1761 CAGCCCCTG
AocI 1287 CCTAAGG CC^T[ACGT]AGG
1322 CCTCAGG
ApoI 699 AAATTC [GA]^AATT[CT]
1487 GAATTC
Asp718I 1404 GGTACC G^GTACC
1635 GGTACC
AvaI 618 CCCGAG C^[CT]CG[GA]G
894 CTCGAG
AviII 502 TGCGCA TGC^GCA
1189 TGCGCA
BbeI 651 GGCGCC GGCGC^C
1273 GGCGCC
BlpI 350 GCTGAGC GC^T[ACGT]AGC
1527 GCTGAGC
BpmI 854 CTCCAG CTGGAG(16/14)
99 CTGGAG
Bpu1102I 350 GCTGAGC GC^T[ACGT]AGC
1527 GCTGAGC
BseRI 375 GAGGAG GAGGAG(10/8)
1182 GAGGAG
BsoBI 618 CCCGAG C^[CT]CG[GA]G
894 CTCGAG
BspMI 131 ACCTGC ACCTGC(4/8)
1009 ACCTGC
BsrFI 204 GCCGGC [GA]^CCGG[CT]
387 GCCGGC
BssHII 429 GCGCGC G^CGCGC
565 GCGCGC
BstEII 623 GGTCACC G^GT[ACGT]ACC
998 GGTGACC
BstPI 623 GGTCACC G^GT[ACGT]ACC
998 GGTGACC
BstXI 628 CCAAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
982 CCAAGGGTGTGG
Bsu36I 1287 CCTAAGG CC^T[ACGT]AGG
1322 CCTCAGG
CelII 350 GCTGAGC GC^T[ACGT]AGC
1527 GCTGAGC
Cfr10I 204 GCCGGC [GA]^CCGG[CT]
387 GCCGGC
Eco81I 1287 CCTAAGG CC^T[ACGT]AGG
1322 CCTCAGG
EcoNI 95 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1037 CCTGACTGAGG
EcoO65I 623 GGTCACC G^GT[ACGT]ACC
998 GGTGACC
FspI 502 TGCGCA TGC^GCA
1189 TGCGCA
KasI 651 GGCGCC G^GCGCC
1273 GGCGCC
KpnI 1404 GGTACC GGTAC^C
1635 GGTACC
NaeI 204 GCCGGC GCC^GGC
387 GCCGGC
NarI 651 GGCGCC GG^CGCC
1273 GGCGCC
NcoI 232 CCATGG C^CATGG
1423 CCATGG
NgoMIV 204 GCCGGC G^CCGGC
387 GCCGGC
PpuMI 1093 AGGACCT [GA]G^G[AT]CC[CT]
1554 GGGACCT
SfoI 651 GGCGCC GGC^GCC
1273 GGCGCC
XcmI 25 CCAGGCCCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
982 CCAAGGGTGTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I ApaLI AspHI AvaII BalI
BanI BanII BbvI BcnI BfaI
BmyI BsaHI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
Tsp509I TspRI Van91I