################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII AccIII AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AviII BbvCI BclI BfrI BglII BlpI Bpu1102I BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspMI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI DraI DraIII DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KspI MamI MluI MroI NdeI NheI NruI NsiI NspV PacI PflMI PinAI PmeI PshBI Psp1406I PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 1479 GGTACC G^GTACC AccII 478 CGCG CG^CG AcyI 625 GGCGCC G[GA]^CG[CT]C AflIII 1415 ACATGT A^C[GA][CT]GT Asp718I 1479 GGTACC G^GTACC AspI 97 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1085 CTCGAG C^[CT]CG[GA]G AvrII 1323 CCTAGG C^CTAGG BalI 635 TGGCCA TGG^CCA BamHI 606 GGATCC G^GATCC BbeI 625 GGCGCC GGCGC^C BbrPI 589 CACGTG CAC^GTG BcgI 45 GCAGCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 1323 CCTAGG C^CTAGG BsaHI 625 GGCGCC G[GA]^CG[CT]C BsaI 415 GAGACC GGTCTC(1/5) BsoBI 1085 CTCGAG C^[CT]CG[GA]G BspHI 1366 TCATGA T^CATGA BspLU11I 1415 ACATGT A^CATGT BsrBI 116 CCGCTC CCGCTC(-3/-3) BsrFI 622 GCCGGC [GA]^CCGG[CT] BssSI 529 CACGAG CACGAG(-5/-1) BstUI 478 CGCG CG^CG BstYI 606 GGATCC [GA]^GATC[CT] Cfr10I 622 GCCGGC [GA]^CCGG[CT] CpoI 558 CGGACCG CG^G[AT]CCG Hin1I 625 GGCGCC G[GA]^CG[CT]C KasI 625 GGCGCC G^GCGCC KpnI 1479 GGTACC GGTAC^C MfeI 1493 CAATTG C^AATTG MflI 606 GGATCC [GA]^GATC[CT] MluNI 635 TGGCCA TGG^CCA MscI 635 TGGCCA TGG^CCA MunI 1493 CAATTG C^AATTG MvnI 478 CGCG CG^CG NaeI 622 GCCGGC GCC^GGC NarI 625 GGCGCC GG^CGCC NcoI 638 CCATGG C^CATGG NgoMIV 622 GCCGGC G^CCGGC PaeR7I 1085 CTCGAG C^TCGAG PflFI 97 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 589 CACGTG CAC^GTG PmlI 589 CACGTG CAC^GTG PshAI 1412 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1366 TCATGA T^CATGA RsrII 558 CGGACCG CG^G[AT]CCG SfoI 625 GGCGCC GGC^GCC SgrAI 621 CGCCGGCG C[GA]^CCGG[CT]G SmlI 1085 CTCGAG C^T[CT][GA]AG SphI 644 GCATGC GCATG^C TfiI 1054 GAATC G^A[AT]TC Tth111I 97 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1085 CTCGAG C^TCGAG XhoII 606 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwNI 572 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 1033 CAGGCGCTG AocI 1075 CCTTAGG CC^T[ACGT]AGG 1738 CCTCAGG BbsI 441 GAAGAC GAAGAC(2/6) 919 GTCTTC BciVI 238 GGATAC GTATCC(6/5) 969 GGATAC BpmI 454 CTGGAG CTGGAG(16/14) 1353 CTGGAG BpuAI 441 GAAGAC GAAGAC(2/6) 919 GTCTTC BsaAI 537 CACGTA [CT]AC^GT[GA] 589 CACGTG BsgI 400 CTGCAC GTGCAG(16/14) 334 GTGCAG BsiEI 62 CGGCCG CG[GA][CT]^CG 393 CGGCCG Bsu36I 1075 CCTTAGG CC^T[ACGT]AGG 1738 CCTCAGG DsaI 638 CCATGG C^C[GA][CT]GG 1294 CCGTGG EagI 62 CGGCCG C^GGCCG 393 CGGCCG EclXI 62 CGGCCG C^GGCCG 393 CGGCCG Eco52I 62 CGGCCG C^GGCCG 393 CGGCCG Eco81I 1075 CCTTAGG CC^T[ACGT]AGG 1738 CCTCAGG HgaI 585 GACGC GACGC(5/10) 479 GCGTC MspA1I 31 CAGCGG C[AC]G^C[GT]G 192 CCGCTG NotI 61 GCGGCCGC GC^GGCCGC 392 GCGGCCGC Tsp509I 1248 AATT ^AATT 1494 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AccI AciI AfaI AluI AlwI AspHI AvaII BanI BanII BbvI BcnI BfaI BglI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI Tru9I TseI Tsp45I TspRI TthHB8I