##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AviII BbvCI BclI
BfrI BglII BlpI Bpu1102I BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspMI BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI DraI DraIII DrdI Eam1105I
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KspI MamI
MluI MroI NdeI NheI NruI
NsiI NspV PacI PflMI PinAI
PmeI PshBI Psp1406I PstI PvuI
PvuII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Van91I XbaI XcmI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 1479 GGTACC G^GTACC
AccII 478 CGCG CG^CG
AcyI 625 GGCGCC G[GA]^CG[CT]C
AflIII 1415 ACATGT A^C[GA][CT]GT
Asp718I 1479 GGTACC G^GTACC
AspI 97 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1085 CTCGAG C^[CT]CG[GA]G
AvrII 1323 CCTAGG C^CTAGG
BalI 635 TGGCCA TGG^CCA
BamHI 606 GGATCC G^GATCC
BbeI 625 GGCGCC GGCGC^C
BbrPI 589 CACGTG CAC^GTG
BcgI 45 GCAGCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1323 CCTAGG C^CTAGG
BsaHI 625 GGCGCC G[GA]^CG[CT]C
BsaI 415 GAGACC GGTCTC(1/5)
BsoBI 1085 CTCGAG C^[CT]CG[GA]G
BspHI 1366 TCATGA T^CATGA
BspLU11I 1415 ACATGT A^CATGT
BsrBI 116 CCGCTC CCGCTC(-3/-3)
BsrFI 622 GCCGGC [GA]^CCGG[CT]
BssSI 529 CACGAG CACGAG(-5/-1)
BstUI 478 CGCG CG^CG
BstYI 606 GGATCC [GA]^GATC[CT]
Cfr10I 622 GCCGGC [GA]^CCGG[CT]
CpoI 558 CGGACCG CG^G[AT]CCG
Hin1I 625 GGCGCC G[GA]^CG[CT]C
KasI 625 GGCGCC G^GCGCC
KpnI 1479 GGTACC GGTAC^C
MfeI 1493 CAATTG C^AATTG
MflI 606 GGATCC [GA]^GATC[CT]
MluNI 635 TGGCCA TGG^CCA
MscI 635 TGGCCA TGG^CCA
MunI 1493 CAATTG C^AATTG
MvnI 478 CGCG CG^CG
NaeI 622 GCCGGC GCC^GGC
NarI 625 GGCGCC GG^CGCC
NcoI 638 CCATGG C^CATGG
NgoMIV 622 GCCGGC G^CCGGC
PaeR7I 1085 CTCGAG C^TCGAG
PflFI 97 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 589 CACGTG CAC^GTG
PmlI 589 CACGTG CAC^GTG
PshAI 1412 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1366 TCATGA T^CATGA
RsrII 558 CGGACCG CG^G[AT]CCG
SfoI 625 GGCGCC GGC^GCC
SgrAI 621 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1085 CTCGAG C^T[CT][GA]AG
SphI 644 GCATGC GCATG^C
TfiI 1054 GAATC G^A[AT]TC
Tth111I 97 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1085 CTCGAG C^TCGAG
XhoII 606 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 572 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1033 CAGGCGCTG
AocI 1075 CCTTAGG CC^T[ACGT]AGG
1738 CCTCAGG
BbsI 441 GAAGAC GAAGAC(2/6)
919 GTCTTC
BciVI 238 GGATAC GTATCC(6/5)
969 GGATAC
BpmI 454 CTGGAG CTGGAG(16/14)
1353 CTGGAG
BpuAI 441 GAAGAC GAAGAC(2/6)
919 GTCTTC
BsaAI 537 CACGTA [CT]AC^GT[GA]
589 CACGTG
BsgI 400 CTGCAC GTGCAG(16/14)
334 GTGCAG
BsiEI 62 CGGCCG CG[GA][CT]^CG
393 CGGCCG
Bsu36I 1075 CCTTAGG CC^T[ACGT]AGG
1738 CCTCAGG
DsaI 638 CCATGG C^C[GA][CT]GG
1294 CCGTGG
EagI 62 CGGCCG C^GGCCG
393 CGGCCG
EclXI 62 CGGCCG C^GGCCG
393 CGGCCG
Eco52I 62 CGGCCG C^GGCCG
393 CGGCCG
Eco81I 1075 CCTTAGG CC^T[ACGT]AGG
1738 CCTCAGG
HgaI 585 GACGC GACGC(5/10)
479 GCGTC
MspA1I 31 CAGCGG C[AC]G^C[GT]G
192 CCGCTG
NotI 61 GCGGCCGC GC^GGCCGC
392 GCGGCCGC
Tsp509I 1248 AATT ^AATT
1494 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AspHI AvaII BanI BanII BbvI
BcnI BfaI BglI BmyI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI Tru9I TseI Tsp45I
TspRI TthHB8I