##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI AspEI
AviII BamHI BbeI BbvCI BclI
BfrI BglII BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsgI BsiWI
BsmBI Bsp1407I BspDI BspEI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DraIII
Eam1105I Eco47III EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FspI HaeII
HpaI KasI KspI MamI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 1445 GGTACC G^GTACC
AccI 1639 GTCTAC GT^[AC][GT]AC
AflIII 422 ACATGT A^C[GA][CT]GT
AocI 122 CCTCAGG CC^T[ACGT]AGG
ApaI 674 GGGCCC GGGCC^C
Asp718I 1445 GGTACC G^GTACC
AspHI 1210 GAGCAC G[AT]GC[AT]^C
AspI 778 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1479 CCTAGG C^CTAGG
BbrPI 1450 CACGTG CAC^GTG
BbsI 351 GAAGAC GAAGAC(2/6)
BcgI 61 CGAGGCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 333 GGATAC GTATCC(6/5)
BlnI 1479 CCTAGG C^CTAGG
BpuAI 351 GAAGAC GAAGAC(2/6)
BsaAI 1450 CACGTG [CT]AC^GT[GA]
BsiHKAI 1210 GAGCAC G[AT]GC[AT]^C
BspLU11I 422 ACATGT A^CATGT
BsrBI 861 CCGCTC CCGCTC(-3/-3)
BsrFI 55 GCCGGC [GA]^CCGG[CT]
BssHII 340 GCGCGC G^CGCGC
BssSI 714 CACGAG CACGAG(-5/-1)
BstAPI 441 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 122 CCTCAGG CC^T[ACGT]AGG
Cfr10I 55 GCCGGC [GA]^CCGG[CT]
Eco81I 122 CCTCAGG CC^T[ACGT]AGG
FseI 54 GGCCGGCC GGCCGG^CC
HincII 1303 GTTGAC GT[CT]^[GA]AC
HindII 1303 GTTGAC GT[CT]^[GA]AC
KpnI 1445 GGTACC GGTAC^C
MseI 1728 TTAA T^TAA
NaeI 55 GCCGGC GCC^GGC
NcoI 1022 CCATGG C^CATGG
NgoMIV 55 GCCGGC G^CCGGC
NspI 422 ACATGT [GA]CATG^[CT]
PflFI 778 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 324 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1450 CACGTG CAC^GTG
PmlI 1450 CACGTG CAC^GTG
PshAI 1306 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 325 CAGCTG CAG^CTG
ScaI 257 AGTACT AGT^ACT
Tru9I 1728 TTAA T^TAA
Tsp45I 763 GTCAC ^GT[GC]AC
Tth111I 778 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 324 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 504 CCAGAAACAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccII 19 CGCG CG^CG
341 CGCG
AcyI 1219 GGCGTC G[GA]^CG[CT]C
1310 GGCGTC
Asp700I 478 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
495 GAAAAAGTTC
AvaI 148 CCCGAG C^[CT]CG[GA]G
554 CCCGAG
BalI 839 TGGCCA TGG^CCA
1019 TGGCCA
BanII 674 GGGCCC G[GA]GC[CT]^C
973 GAGCCC
BsaHI 1219 GGCGTC G[GA]^CG[CT]C
1310 GGCGTC
BsiEI 53 CGGCCG CG[GA][CT]^CG
57 CGGCCG
BsmFI 23 GTCCC GGGAC(10/14)
134 GTCCC
BsmI 1539 GAATGC GAATGC(1/-1)
482 GCATTC
BsoBI 148 CCCGAG C^[CT]CG[GA]G
554 CCCGAG
BspHI 95 TCATGA T^CATGA
1223 TCATGA
BsrDI 67 CATTGC GCAATG(2/0)
1344 CATTGC
BstUI 19 CGCG CG^CG
341 CGCG
BstXI 891 CCAGAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1733 CCAGTGATGTGG
BstYI 443 AGATCC [GA]^GATC[CT]
1411 GGATCT
DrdI 775 GACGACCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1192 GACCTTATTGTC
DsaI 1022 CCATGG C^C[GA][CT]GG
1349 CCACGG
EagI 53 CGGCCG C^GGCCG
57 CGGCCG
EclXI 53 CGGCCG C^GGCCG
57 CGGCCG
Eco52I 53 CGGCCG C^GGCCG
57 CGGCCG
EcoT14I 1022 CCATGG C^C[AT][AT]GG
1479 CCTAGG
FauI 860 CCCGC CCCGC(4/6)
1010 CCCGC
Hin1I 1219 GGCGTC G[GA]^CG[CT]C
1310 GGCGTC
HindIII 1174 AAGCTT A^AGCTT
1426 AAGCTT
MaeII 1451 ACGT A^CGT
1472 ACGT
MaeIII 763 GTCAC ^GT[ACGT]AC
1160 GTTAC
MflI 443 AGATCC [GA]^GATC[CT]
1411 GGATCT
MluNI 839 TGGCCA TGG^CCA
1019 TGGCCA
MscI 839 TGGCCA TGG^CCA
1019 TGGCCA
MspA1I 325 CAGCTG C[AC]G^C[GT]G
364 CAGCGG
MvnI 19 CGCG CG^CG
341 CGCG
PpuMI 132 GGGTCCC [GA]G^G[AT]CC[CT]
1613 AGGACCC
PstI 439 CTGCAG CTGCA^G
490 CTGCAG
RcaI 95 TCATGA T^CATGA
1223 TCATGA
StyI 1022 CCATGG C^C[AT][AT]GG
1479 CCTAGG
TaqI 372 TCGA T^CGA
486 TCGA
TfiI 369 GAATC G^A[AT]TC
1282 GATTC
TthHB8I 372 TCGA T^CGA
486 TCGA
XhoII 443 AGATCC [GA]^GATC[CT]
1411 GGATCT
XmnI 478 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
495 GAAAAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MboI MboII MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TseI Tsp509I TspRI