################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BbeI BbrPI BbsI BbvCI BcgI BclI BglII BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AflIII 1419 ACGTGT A^C[GA][CT]GT AocI 1645 CCTGAGG CC^T[ACGT]AGG Aor51HI 733 AGCGCT AGC^GCT AvaI 143 CCCGAG C^[CT]CG[GA]G BamHI 1408 GGATCC G^GATCC BcnI 1247 CCGGG CC^[GC]GG BpmI 868 CTGGAG CTGGAG(16/14) BsaAI 1418 TACGTG [CT]AC^GT[GA] BseRI 1857 GAGGAG GAGGAG(10/8) BsoBI 143 CCCGAG C^[CT]CG[GA]G BsrBI 1412 CCGCTC CCGCTC(-3/-3) BsrDI 308 CATTGC GCAATG(2/0) BsrFI 66 GCCGGC [GA]^CCGG[CT] BssSI 118 CACGAG CACGAG(-5/-1) BstAPI 909 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1645 CCTGAGG CC^T[ACGT]AGG Cfr10I 66 GCCGGC [GA]^CCGG[CT] DraI 1365 TTTAAA TTT^AAA DsaI 1563 CCATGG C^C[GA][CT]GG Eco47III 733 AGCGCT AGC^GCT Eco81I 1645 CCTGAGG CC^T[ACGT]AGG EcoNI 1362 CCTTTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 43 GAATTC G^AATTC FauI 109 GCGGG CCCGC(4/6) MaeII 1419 ACGT A^CGT NaeI 66 GCCGGC GCC^GGC NciI 1247 CCGGG CC^[GC]GG NcoI 1563 CCATGG C^CATGG NgoMIV 66 GCCGGC G^CCGGC NspI 1021 GCATGC [GA]CATG^[CT] SfiI 924 GGCCTCAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 1021 GCATGC GCATG^C StuI 15 AGGCCT AGG^CCT Tsp45I 1482 GTCAC ^GT[GC]AC XbaI 1853 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 43 GAATTC [GA]^AATT[CT] 711 GAATTT AflII 228 CTTAAG C^TTAAG 1286 CTTAAG ApoI 43 GAATTC [GA]^AATT[CT] 711 GAATTT AvrII 367 CCTAGG C^CTAGG 1400 CCTAGG BanI 333 GGCACC G^G[CT][GA]CC 1548 GGCACC BciVI 323 GGATAC GTATCC(6/5) 878 GTATCC BfrI 228 CTTAAG C^TTAAG 1286 CTTAAG BglI 95 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 925 GCCTCAAAGGC BlnI 367 CCTAGG C^CTAGG 1400 CCTAGG BsgI 1681 CTGCAC GTGCAG(16/14) 907 GTGCAG BsmFI 1775 GGGAC GGGAC(10/14) 1277 GTCCC BspMI 473 ACCTGC ACCTGC(4/8) 1490 GCAGGT DraII 1582 GGGTCCT [GA]G^G[ACGT]CC[CT] 1775 GGGACCC EcoO109I 1582 GGGTCCT [GA]G^G[ACGT]CC[CT] 1775 GGGACCC HaeII 153 AGCGCC [GA]GCGC^[CT] 733 AGCGCT HapII 67 CCGG C^CGG 1247 CCGG HpaII 67 CCGG C^CGG 1247 CCGG MslI 749 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 818 CATCAAAGTG MspA1I 627 CAGCTG C[AC]G^C[GT]G 959 CAGCTG MspI 67 CCGG C^CGG 1247 CCGG PleI 1041 GAGTC GAGTC(4/5) 1480 GAGTC PpuMI 1582 GGGTCCT [GA]G^G[AT]CC[CT] 1775 GGGACCC PstI 1604 CTGCAG CTGCA^G 1656 CTGCAG PvuII 627 CAGCTG CAG^CTG 959 CAGCTG ScaI 464 AGTACT AGT^ACT 1554 AGTACT TaqI 236 TCGA T^CGA 1060 TCGA TthHB8I 236 TCGA T^CGA 1060 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI Asp700I AspHI AvaII BalI BanII BbvI BfaI BlpI BmyI Bpu1102I BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp509I TspRI XhoII XmnI