##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII BbeI
BbrPI BbsI BbvCI BcgI BclI
BglII BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 1419 ACGTGT A^C[GA][CT]GT
AocI 1645 CCTGAGG CC^T[ACGT]AGG
Aor51HI 733 AGCGCT AGC^GCT
AvaI 143 CCCGAG C^[CT]CG[GA]G
BamHI 1408 GGATCC G^GATCC
BcnI 1247 CCGGG CC^[GC]GG
BpmI 868 CTGGAG CTGGAG(16/14)
BsaAI 1418 TACGTG [CT]AC^GT[GA]
BseRI 1857 GAGGAG GAGGAG(10/8)
BsoBI 143 CCCGAG C^[CT]CG[GA]G
BsrBI 1412 CCGCTC CCGCTC(-3/-3)
BsrDI 308 CATTGC GCAATG(2/0)
BsrFI 66 GCCGGC [GA]^CCGG[CT]
BssSI 118 CACGAG CACGAG(-5/-1)
BstAPI 909 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1645 CCTGAGG CC^T[ACGT]AGG
Cfr10I 66 GCCGGC [GA]^CCGG[CT]
DraI 1365 TTTAAA TTT^AAA
DsaI 1563 CCATGG C^C[GA][CT]GG
Eco47III 733 AGCGCT AGC^GCT
Eco81I 1645 CCTGAGG CC^T[ACGT]AGG
EcoNI 1362 CCTTTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 43 GAATTC G^AATTC
FauI 109 GCGGG CCCGC(4/6)
MaeII 1419 ACGT A^CGT
NaeI 66 GCCGGC GCC^GGC
NciI 1247 CCGGG CC^[GC]GG
NcoI 1563 CCATGG C^CATGG
NgoMIV 66 GCCGGC G^CCGGC
NspI 1021 GCATGC [GA]CATG^[CT]
SfiI 924 GGCCTCAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1021 GCATGC GCATG^C
StuI 15 AGGCCT AGG^CCT
Tsp45I 1482 GTCAC ^GT[GC]AC
XbaI 1853 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 43 GAATTC [GA]^AATT[CT]
711 GAATTT
AflII 228 CTTAAG C^TTAAG
1286 CTTAAG
ApoI 43 GAATTC [GA]^AATT[CT]
711 GAATTT
AvrII 367 CCTAGG C^CTAGG
1400 CCTAGG
BanI 333 GGCACC G^G[CT][GA]CC
1548 GGCACC
BciVI 323 GGATAC GTATCC(6/5)
878 GTATCC
BfrI 228 CTTAAG C^TTAAG
1286 CTTAAG
BglI 95 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
925 GCCTCAAAGGC
BlnI 367 CCTAGG C^CTAGG
1400 CCTAGG
BsgI 1681 CTGCAC GTGCAG(16/14)
907 GTGCAG
BsmFI 1775 GGGAC GGGAC(10/14)
1277 GTCCC
BspMI 473 ACCTGC ACCTGC(4/8)
1490 GCAGGT
DraII 1582 GGGTCCT [GA]G^G[ACGT]CC[CT]
1775 GGGACCC
EcoO109I 1582 GGGTCCT [GA]G^G[ACGT]CC[CT]
1775 GGGACCC
HaeII 153 AGCGCC [GA]GCGC^[CT]
733 AGCGCT
HapII 67 CCGG C^CGG
1247 CCGG
HpaII 67 CCGG C^CGG
1247 CCGG
MslI 749 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
818 CATCAAAGTG
MspA1I 627 CAGCTG C[AC]G^C[GT]G
959 CAGCTG
MspI 67 CCGG C^CGG
1247 CCGG
PleI 1041 GAGTC GAGTC(4/5)
1480 GAGTC
PpuMI 1582 GGGTCCT [GA]G^G[AT]CC[CT]
1775 GGGACCC
PstI 1604 CTGCAG CTGCA^G
1656 CTGCAG
PvuII 627 CAGCTG CAG^CTG
959 CAGCTG
ScaI 464 AGTACT AGT^ACT
1554 AGTACT
TaqI 236 TCGA T^CGA
1060 TCGA
TthHB8I 236 TCGA T^CGA
1060 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
Asp700I AspHI AvaII BalI BanII
BbvI BfaI BlpI BmyI Bpu1102I
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MvaI
MwoI NdeII NlaIII NlaIV RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp509I TspRI XhoII
XmnI