##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII BamHI BbrPI
BbvCI BcgI BfrI BlpI Bpu1102I
BsaAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KpnI MfeI MluI
MunI NcoI NdeI NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SalI SbfI ScaI SfcI
SfiI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 156 TCCGGA T^CCGGA
AcsI 1181 GAATTC [GA]^AATT[CT]
ApoI 1181 GAATTC [GA]^AATT[CT]
Asp700I 871 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 76 CCTAGG C^CTAGG
BbeI 91 GGCGCC GGCGC^C
BclI 981 TGATCA T^GATCA
BlnI 76 CCTAGG C^CTAGG
BsaI 1619 GAGACC GGTCTC(1/5)
BseAI 156 TCCGGA T^CCGGA
BsgI 279 CTGCAC GTGCAG(16/14)
BsiEI 1329 CGGCCG CG[GA][CT]^CG
BspEI 156 TCCGGA T^CCGGA
BspMI 1778 ACCTGC ACCTGC(4/8)
BsrBI 55 GAGCGG CCGCTC(-3/-3)
BsrFI 1782 GCCGGC [GA]^CCGG[CT]
BstAPI 724 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1209 TTCGAA TT^CGAA
BstXI 339 CCATCGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1782 GCCGGC [GA]^CCGG[CT]
DraIII 282 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1329 CGGCCG C^GGCCG
EclXI 1329 CGGCCG C^GGCCG
Eco52I 1329 CGGCCG C^GGCCG
EcoRI 1181 GAATTC G^AATTC
EcoT14I 76 CCTAGG C^C[AT][AT]GG
FbaI 981 TGATCA T^GATCA
HaeII 91 GGCGCC [GA]GCGC^[CT]
KasI 91 GGCGCC G^GCGCC
MroI 156 TCCGGA T^CCGGA
MseI 1160 TTAA T^TAA
NaeI 1782 GCCGGC GCC^GGC
NarI 91 GGCGCC GG^CGCC
NgoMIV 1782 GCCGGC G^CCGGC
NspV 1209 TTCGAA TT^CGAA
PvuII 276 CAGCTG CAG^CTG
SapI 1453 GAAGAGC GCTCTTC(1/4)
SfoI 91 GGCGCC GGC^GCC
SfuI 1209 TTCGAA TT^CGAA
SmlI 1517 CTCAAG C^T[CT][GA]AG
StuI 1872 AGGCCT AGG^CCT
StyI 76 CCTAGG C^C[AT][AT]GG
TaqI 1210 TCGA T^CGA
Tru9I 1160 TTAA T^TAA
TthHB8I 1210 TCGA T^CGA
XbaI 1927 TCTAGA T^CTAGA
XmnI 871 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 91 GGCGCC G[GA]^CG[CT]C
105 GACGCC
AflIII 411 ACATGT A^C[GA][CT]GT
1290 ACATGT
AlwI 943 GATCC GGATC(4/5)
1123 GATCC
AspHI 1145 GAGCAC G[AT]GC[AT]^C
1381 GTGCTC
BalI 169 TGGCCA TGG^CCA
357 TGGCCA
BanI 91 GGCGCC G^G[CT][GA]CC
806 GGCACC
BciVI 746 GGATAC GTATCC(6/5)
547 GTATCC
BglI 39 GCCCCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
461 GCCCCTGGGGC
BsaBI 1117 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1594 GATTCGCATC
BsaHI 91 GGCGCC G[GA]^CG[CT]C
105 GACGCC
BsiHKAI 1145 GAGCAC G[AT]GC[AT]^C
1381 GTGCTC
BspLU11I 411 ACATGT A^CATGT
1290 ACATGT
BsrDI 1140 GCAATG GCAATG(2/0)
1740 GCAATG
HgaI 105 GACGC GACGC(5/10)
153 GCGTC
Hin1I 91 GGCGCC G[GA]^CG[CT]C
105 GACGCC
MamI 1117 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1594 GATTCGCATC
MluNI 169 TGGCCA TGG^CCA
357 TGGCCA
MscI 169 TGGCCA TGG^CCA
357 TGGCCA
MslI 1279 CACAAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1600 CATCATGGTG
PflMI 284 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
707 CCACTCCCTGG
PshAI 1045 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1915 GACCTGGGTC
SexAI 283 ACCAGGT A^CC[AT]GGT
1401 ACCTGGT
SmaI 100 CCCGGG CCC^GGG
751 CCCGGG
SphI 330 GCATGC GCATG^C
1494 GCATGC
Tsp509I 1158 AATT ^AATT
1182 AATT
Van91I 284 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
707 CCACTCCCTGG
XmaCI 100 CCCGGG C^CCGGG
751 CCCGGG
XmaI 100 CCCGGG C^CCGGG
751 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI TfiI TseI Tsp45I
TspRI XhoII