##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AvaI AvrII
BamHI BbeI BbrPI BcgI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraII EagI EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI FbaI FseI HpaI KasI
KpnI MamI MfeI MluI MroI
MslI MunI NarI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI Tth111I Van91I
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcyI 154 GACGCC G[GA]^CG[CT]C
AflIII 1423 ACACGT A^C[GA][CT]GT
AhdI 1333 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1333 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 68 TGCGCA TGC^GCA
BanI 1365 GGTGCC G^G[CT][GA]CC
BbvCI 108 CCTCAGC CCTCAGC(-5/-2)
BciVI 940 GTATCC GTATCC(6/5)
BglII 558 AGATCT A^GATCT
BsaHI 154 GACGCC G[GA]^CG[CT]C
BsaI 772 GAGACC GGTCTC(1/5)
BspMI 681 ACCTGC ACCTGC(4/8)
BsrBI 33 GAGCGG CCGCTC(-3/-3)
BsrFI 532 GCCGGC [GA]^CCGG[CT]
BstXI 1703 CCAGCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 558 AGATCT [GA]^GATC[CT]
Cfr10I 532 GCCGGC [GA]^CCGG[CT]
DrdI 353 GACAAAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1333 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1369 CCTTCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 639 CCAAGG C^C[AT][AT]GG
EcoT22I 283 ATGCAT ATGCA^T
FspI 68 TGCGCA TGC^GCA
HaeII 144 GGCGCT [GA]GCGC^[CT]
HgaI 154 GACGC GACGC(5/10)
Hin1I 154 GACGCC G[GA]^CG[CT]C
HincII 1593 GTTGAC GT[CT]^[GA]AC
HindII 1593 GTTGAC GT[CT]^[GA]AC
HindIII 720 AAGCTT A^AGCTT
KspI 119 CCGCGG CCGC^GG
MaeII 1425 ACGT A^CGT
MflI 558 AGATCT [GA]^GATC[CT]
NaeI 532 GCCGGC GCC^GGC
NgoMIV 532 GCCGGC G^CCGGC
NsiI 283 ATGCAT ATGCA^T
PshAI 1171 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1079 CAGCTG CAG^CTG
SacI 492 GAGCTC GAGCT^C
SacII 119 CCGCGG CCGC^GG
SapI 1208 GAAGAGC GCTCTTC(1/4)
SexAI 935 ACCAGGT A^CC[AT]GGT
StyI 639 CCAAGG C^C[AT][AT]GG
SwaI 1797 ATTTAAAT ATTT^AAAT
XbaI 1095 TCTAGA T^CTAGA
XhoII 558 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 26 GTCTAC GT^[AC][GT]AC
271 GTAGAC
AccII 1 CGCG CG^CG
120 CGCG
AlwNI 613 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
1243 CAGAGGCTG
AspHI 492 GAGCTC G[AT]GC[AT]^C
1312 GAGCAC
BalI 382 TGGCCA TGG^CCA
571 TGGCCA
BanII 492 GAGCTC G[GA]GC[CT]^C
621 GGGCTC
BseRI 477 GAGGAG GAGGAG(10/8)
489 GAGGAG
BsiHKAI 492 GAGCTC G[AT]GC[AT]^C
1312 GAGCAC
BsmI 285 GCATTC GAATGC(1/-1)
1706 GCATTC
BsrDI 509 CATTGC GCAATG(2/0)
1004 GCAATG
BstUI 1 CGCG CG^CG
120 CGCG
DpnII 559 GATC ^GATC
1731 GATC
DraI 1498 TTTAAA TTT^AAA
1798 TTTAAA
DraIII 689 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1424 CACGTCGTG
DsaI 119 CCGCGG C^C[GA][CT]GG
174 CCACGG
EaeI 382 TGGCCA [CT]^GGCC[GA]
571 TGGCCA
EcoRV 296 GATATC GAT^ATC
835 GATATC
MboI 559 GATC ^GATC
1731 GATC
MluNI 382 TGGCCA TGG^CCA
571 TGGCCA
MscI 382 TGGCCA TGG^CCA
571 TGGCCA
MvnI 1 CGCG CG^CG
120 CGCG
NdeII 559 GATC ^GATC
1731 GATC
NspI 990 ACATGC [GA]CATG^[CT]
1213 GCATGT
Sau3AI 559 GATC ^GATC
1731 GATC
SmlI 276 CTTGAG C^T[CT][GA]AG
497 CTTGAG
TaqI 363 TCGA T^CGA
563 TCGA
TfiI 207 GAATC G^A[AT]TC
1441 GAATC
TthHB8I 363 TCGA T^CGA
563 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI EarI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboII MnlI
MseI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PleI PstI
RsaI Sau96I ScrFI SfaNI SfcI
Tru9I TseI Tsp45I Tsp509I TspRI